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Artikel T. Paniculatum - Icowobas
Artikel T. Paniculatum - Icowobas
Abstract
Java ginseng (Talinum paniculatum Gaertn.) root was reported to have efficacy similar to the Korean ginseng (Panax
ginseng) root. Hairy root induction of Java ginseng on free hormone solid medium Murashige and Skoog (MS) using
genetic transformation mediated by Agrobacterium rhizogenes had succesfully performed. 2 - 3 cm in lenght of hairy
roots were initiated from cut edge of leaves after two weeks incubation and were used for subculture experiments on
solid and liquid MS medium without hormone. Subcultures were performed 4 times for two weeks period. Final
incubation of the fourth subculture showed differences of medium type (solid and liqiud) affected hairy root
development. Hairy roots on solid MS medium grew straight and had no brancing, whereas hairy roots on liquid
medium had branching and forming ball like structures (2-3 structures/cm). Hairy roots which were subcultured on
liquid medium looked thicker than on solid medium. The thickness of hairy root, branching and formation ball like
structure indicated suitability liquid medium as subculture medium than solid medium.
Keywords: hairy root, subculture, Talinum paniculatum Gaertn.
1.
Introduction
2.
3.
5 mm
M 40X
rh
el
M 100X
mr
mt
Figure 1. Hairy root morphology was subcultured on solid MS medium without hormone. Subculture was
performed 4 times for 2 weeks period; A. whole hairy root at magnify 40x; B. zoom of part of
hairy root with red line mark at magnify 100x; el.elongation zone; mr. meristematic zone;
mt. maturation zone; and rh. root hair.
rh
bl
rb
M 40X
5 mm
B1
B2
mt
rb
rb
el
mr
mr
M 100X
M 100X
B4
B3
bl
bl
M 100X
M 100X
Figure 2. Hairy root morphology was subcultured on liquid MS medium without hormone. Subculture was
performed 4 times for 2 weeks period; A. whole hairy root at magnify 40x; B. zoom of part of hairy root with
red (1), blue (2), orange (3) and green (4) line mark at magnify 100x; el.elongation zone; mr. meristematic
zone; mt. maturation zone; bl. ball like structure; rb. root branching; and rh. root hair.
4.
Conclusion
References
[1] Adelberg, J. 2008. Agitated, Thin-Film of
Liquid Media for Efficient Micropopagation.
In: Plant Tissue Culture Engineering. Eds.
Gupta, S.D and Ibaraki, Y. Springer,
Netherland.
[2] Choi, Y.E., Kim, Y.S and Paek, K.Y. 2008.
Types and Design of Bioreactor for Hairy
Root Culture. In : Plant Tissue culture
engineering. Eds. Gupta, S.D and Ibaraki, Y.
Springer, Netherland.
[3] Court W.E. 2006. Ginseng The Genus
Panax. Hardwood Academic Publisher.
[4] Curtis, W.R. 2005. Application of
Bioreactor Design Principles to Plant
Micropopagation. In : Liquid Culture System
for in vitro Plant Propagation. Eds. EideHvoslef, K.A and Preil, W. Springer,
Netherland.
[5] Douglas, G.C. 1984. Propagation of Eight
Cultivar of Rhododendron in vitro Using
Agar-Solidified and Liquid Media and
Direct Rooting of Shoot in vivo. Scientia
Horticulturae. 24:337-347.
[6] Eibl, R and Eibl, D. 2008. Design and Use
of the wave Bioreactor for Plant Cell
Culture. In: Plant Tissue Culture
Engineering. Eds. Gupta, S.D and Ibaraki,
Y. Springer, Netherland.
[7] Hu, Zhi-Bi and Du Min. 2006. Hairy Root
and Its Application in Plant genetic
Engineering. Journal of Integrative Plant
Biology. 48:121-127.
[8] Leifert, C., Murphy, K.P and Lumsden, P.J.
1995. Mineral and carbohydrate nutrition of
plant cell and tissue cultures. Plant Sci. 14:
83-109.
[9] Preil, W. 2005. General Introduction: a
personal reflection on the use of liquid
media for in fitro culture. In: Liquid Culture
System for in vitro Plant Propagation. Eds.
Eide-Hvoslef, K.A and Preil, W. Springer,
Netherland.