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  • BAM R31: Glycerin Salt Solution (Buffered) Bacteriological Analytical Manual

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    This document provides instructions for making a buffered glycerin-salt solution according to the Bacteriological Analytical Manual (BAM) R31 method. The solution contains glycerin, potassium phosphate, monosodium phosphate, and sodium chloride dissolved in distilled water. The pH is adjusted to 7.2 and the solution is autoclaved at 121°C for 15 minutes. A double strength solution can also be made using 200 ml of glycerin and 800 ml of water. The original source of the method is the 8th Edition, Revision A of the BAM from 1998.

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    lab method

    Original Title

    Laboratory Methods _ BAM R31_ Glycerin-Salt Solution (Buffered)

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    This document provides instructions for making a buffered glycerin-salt solution according to the Bacteriological Analytical Manual (BAM) R31 method. The solution contains glycerin, potassium phosphate, monosodium phosphate, and sodium chloride dissolved in distilled water. The pH is adjusted to 7.2 and the solution is autoclaved at 121°C for 15 minutes. A double strength solution can also be made using 200 ml of glycerin and 800 ml of water. The original source of the method is the 8th Edition, Revision A of the BAM from 1998.

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    16 views1 page

    BAM R31: Glycerin Salt Solution (Buffered) Bacteriological Analytical Manual

    Uploaded by

    anil
    This document provides instructions for making a buffered glycerin-salt solution according to the Bacteriological Analytical Manual (BAM) R31 method. The solution contains glycerin, potassium phosphate, monosodium phosphate, and sodium chloride dissolved in distilled water. The pH is adjusted to 7.2 and the solution is autoclaved at 121°C for 15 minutes. A double strength solution can also be made using 200 ml of glycerin and 800 ml of water. The original source of the method is the 8th Edition, Revision A of the BAM from 1998.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 1

    9/29/2016

    LaboratoryMethods>BAMR31:GlycerinSaltSolution(Buffered)

    BAMR31:GlycerinSalt
    Solution(Buffered)
    January2001

    BacteriologicalAnalytical
    Manual
    R31
    GlycerinSaltSolution(Buffered)
    Glycerin(reagentgrade) 100ml
    K2HPO4(anhydrous)

    12.4g

    KH2PO4(anhydrous)

    4g

    NaCl
    Distilledwater

    4.2g
    900ml

    DistilledNaClandbringvolumeto900mlwithwater.Addglycerinandphosphates.
    AdjustpHto7.2.Autoclave15minat121C.Fordoublestrength(20%)glycerin
    solution,use200mlglycerinand800mldistilledwater.

    OriginalSource:BacteriologicalAnalyticalManual,8thEdition,RevisionA,1998.
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