LDH-P

DGKC-(5+1)

PROCEDURE
164
164
164
164
164

Cat.No
216
200
203
201 HIT I
202 HIT II

7
5
4
4
4

x
x
x
x
x

Package Size
10 mL
2 x 7 mL
20 mL
1 x 20 mL
100 mL 4 x 20 mL
50 mL
2 x 20 mL
100 mL 4 x 20 mL

METHOD
Enzymatic, Kinetic
PRINCIPLE
LDH
Pyruvate + NADH > Lactate + NAD+

REAGENTS
COMPOSITION (Concentrations in the test) :
Reagent R1:
TRIS-Buffer pH 7.5
50mmol/L
Pyruvate
0.6mmol/L
Sodium Azide
0.09%
Reagent R2 (Starter reagent):
NADH
0.18mmol/L
STABILITY
The sealed reagents are stable up to the indicated
expiry date if stored at +2 to +8C.
PREPARATION
R1 and R2 are ready for use
Stability after opening :
at least 3 months
at +2 to +8 C , if contamination is avoided
Working Reagent (WKR)
Mix 5 parts of reagent R1 with 1 part of R2
Stability: at +2 to +8 C: 8 days
at +18 to +25 C: 2 days

Wavelength :
Light path:
Temperature :

334/340/365nm
1cm
37 C

Pipette into cuvettes:

Serumstart
WKR
1000L
R1
Sample
20L
R2
-

Start with R2
1000L
20L
200L

Mix well immediately after addition of the sample

(Serumstart) respectively R2 (R2-start).
Measure the absorbance after 30sec. Repeat
measurement every minute for 3 minutes.
Calculate the differences in absorbance/min
(A/min)

CALCULATION
with Factor
A /min x Factor = LDH-Activity [U/l]
Factor for Serumstart:
340 nm
8095
334 nm
8250
365 nm
15000
Factor for Start with reagent R2 :
340 nm
9683
334 nm
9870
365 nm
17940

CALIBRATORS and CONTROLS

For the calibration of automated analyzers Greiner
Multicalibrator is recommended, for quality control
use Greiner normal and abnormal controls Unitrol I
and Unitrol II .

SAMPLE MATERIAL
Serum, heparin-/EDTA-plasma

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Greiner Diagnostic GmbH - Unter Gereuth 10 D-79353 Bahlingen Germany

www.greiner-diagnostic.com

REFERENCE VALUES

PERFORMANCE DATA (37C)

Analytical range
The reagent is linear up to 1800 U/L at 340 nm
At higher values the measurement has to be
repeated after dilution of the sample (1 part) with
phys. NaCl solution (10 parts). In this case multiply
the result by 11.

Note: It is recommended for each laboratory to

establish and maintain its own reference values. The
given data here are only an indication.

Sensitivity
The detection limit is 2,42 U/L

BIBLIOGRAPHY

Expected range:
Adults: 240-480 U/L

(4.00-8.00kat/L)

- Specificity
When a control value of known concentration is
assayed, the assay value falls within the range of
<10% of the known concentration.

Weihaar, D. et al.: Med. Welt 26 (1975), 387

Z. klin. Chem. u. Biochem. 8 (1970) 658 and 10
(1972), 182.

- Precision
When the indicated number of samples is
assayed, the following CV values are obtained:

SYMBOLS USED

Within run
n = 11
Sample 1
Sample 2
Sample 3

Mean
[U/L]
300
491
368

SD
[U/L]
4.56
2.25
4.29

CV
[%]
1.52
0.46
1.17

Day to day
n = 11
Sample 1
Sample 2
Sample 3

Mean
[U/L]
303
491
352

SD
[U/L]
4.56
2.95
3.65

CV
[%]
1.50
0.60
1.04

For in vitro diagnostic medical use

Batch Code

Use by
Temperature limitation

Correlation
A comparative study has been performed between
the Greiner method and another commercial
reagent on 29 human serum samples. The
parameters of linear regression are as follows:
Correlation coefficient
Linear regression:

r = 0.9956
y = 1.019 x + 2.653 U/L

INTERFERENCES
-Ascorbic Acid:
-Triglycerides:
-Bilirubin:
-Hemoglobin:

No interference up to 50 mg/L
No interference up to 1000 mg/dL
no interference up to 40 mg / dL
Interference through LDH from
the erythrocytes

08/2010//rev03/2014

Page 2/2

Greiner Diagnostic GmbH - Unter Gereuth 10 D-79353 Bahlingen Germany

www.greiner-diagnostic.com