4.1.

2 Protein Factories Paragraph

The goal of this experiment is to grow E.Coli bacteria and implant them
with the pGLO plasmid from a jellyfish, Aequorea victoria, that will make
them have a gene that produces a green fluorescent protein that glows in
the dark. In the lab, we took a negative pGLO control and a positive pGLO
control and put them in different environments to see if they will grow and if
they will glow. To get the plasmid DNA inside the E.Coli cells, we used a heat
shock (or a chemical transformation) so that the cell membrane will open
and the new plasmid can get in. The new plasmid will give the cell a
resistance to ampicillin. In the presence of arabinose, the cells will glow
green under ultraviolet light.