Name: EDITH PARI LLANOS

Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. Which of the following is/are true of bile?
You correctly answered: b. It works by a physical process.
2. The substrate used in this simulation is
You correctly answered: c. vegetable oil.
3. When fatty acids are liberated by lipase, the pH
You correctly answered: b. decreases.
4. One of the products of the chemical digestion of lipids is
You correctly answered: d. fatty acids.

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Experiment Results
Predict Question:
Predict Question: Which tube do you think will have the highest lipase activity?
Your answer : d. tube 5
Stop & Think Questions:
What does the pH measure?
You correctly answered: d. both lipase activity and fatty acid release
Why is it difficult to detect whether lipase is active in tube 5?
You correctly answered: d. The pH is already very low, so a decrease in pH might be difficult to detect.
Experiment Data:
Tube No.
1
2

Reagent 1
Lipase
Lipase

Reagent 2
Vegetable Oil
Vegetable Oil

3
4
5
6

Lipase
Deionized Water
Lipase
Lipase

Deionized Water
Vegetable Oil
Vegetable Oil
Vegetable Oil

Reagent 3
Bile Salts
Deionized
Water
Bile Salts
Bile Salts
Bile Salts
Bile Salts

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Reagent 4
pH 7.0 Buffer
pH 7.0 Buffer

Time
60
60

Temp.
37
37

pH
6.21
7.00

pH 9.0 Buffer
pH 7.0 Buffer
pH 2.0 Buffer
pH 9.0 Buffer

60
60
60
60

37
37
37
37

9.00
7.00
2.00
9.00

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Post-lab Quiz Results

You scored 100% by answering 4 out of 4 questions correctly.
1. What is the product of lipase hydrolysis?
You correctly answered: d. fatty acids
2. From your results, which pH is ideal for pancreatic lipase digestion?
You correctly answered: b. pH 7.0
3. Which tube confirms that there is no lipase in bile salts or vegetable oil?
You correctly answered: c. tube 4
4. From your results, where (in theory) would pancreatic lipase be active?
You correctly answered: d. mouth and pancreas

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Review Sheet Results

1. Explain why you can't fully test the lipase activity in tube 5.
Your answer:
La actividad de la lipasa en tubo 5 es dificil de detectar , debido a que se utilizo un pH bajo el cual fue de 2 por lo que la
disminucin en el pH no se sabe si es por.el pH utilizado o por la actividad de la lipasa y liberacion de acidos grasos
2. Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible
reasons why it may or may not have matched.
Your answer:
La mayor actividad de la lipasa se dio en el tubo 1 , cuyo contenido fue : lipasa , aceite vegetal y sales biliares . todo esto
con un ph de 7 . Al terminar la incubacion , se midio el ph de la solucion el cual fue de 6.21 lo cual indica que que hubo
activiadad de la lipasa y existio liberacion de acidos grasos .
3. Explain why pancreatic lipase would be active in both the mouth and the pancreas.
Your answer:
La lipasa hidroliza cada triglicerido dando un monoglicerido y dos acidos grasos La digestin de las grasas comienza en la
boca con la secrecin de lipasa bucal, un componente de la saliva la cual ayuda digiriendo pequeas cantidades de lipidos.
La lipasa es mas activa en el intestino delgado puesto que aqui es ayudado por las sales biliares. La actividad es optima
debido que en ambos lados actua con un pH de 7 .
4. Describe the process of bile emulsification of lipids and how it improves lipase activity.
Your answer:
EL proceso de emulsion fisica de los lipidos se da en el intestino delgado por parte de las sales biliares las cuales son
secretadas con el fin de separar los acumulos de lipidos y aumentar la superficie accesible a la enzima lipasa , actua como
detergente . Como resultado de la reaccion produce Pequeas gotas de trigliceridos
Los cuales son hidrolizados por la lipasa dando lugar a un monoaciloglicerido y 2 acidos grasos .

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