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Edexcel Biology As Core Practical Workbook PDF
Edexcel Biology As Core Practical Workbook PDF
Edexcel Biology As Core Practical Workbook PDF
Biology A-Level
Core Practical Workbook
Edexcel Specification
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1.2
2.1
2.2
3.1
Observing mitosis
3.2
4.1
4.2
4.3
The factor
independent
measure.
Reliability:
Accuracy:
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that is
variable.
affected by
The factor
the
you
Validity:
Random Error:
Systematic Error:
Null Hypothesis:
Experimental Hypothesis:
Your working hypothesis that the
independent variable does have an effect on
the dependent variable. By disproving the
Null Hypothesis you can accept your
Experimental Hypothesis1.
Note: it is virtually impossible to prove something correct, yet very simple to prove something
incorrect. Therefore, scientists aim to disprove their Null Hypothesis, which then allows them to accept
their Experimental Hypothesis according to the principle of Occams Razor.
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Learning Outcome
3) Use appropriate
methodology, including ICT,
to answer scientific
questions and solve
scientific problems
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Practical
Criteria
Learning Outcome
6) Evaluate methodology,
evidence and data, and
resolve conflicting evidence
8) Communicate information
and ideas in appropriate
ways using appropriate
terminology
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Practical
Criteria
Learning Outcome
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Practical
Method:
Caffeine solution
Cotton wool
Pipettes
Test tubes
Stopclock
Paper towels or filter paper
Microscope (+ lamp if
needed)
heart
gut
antenna
eye
legs
Daphnia
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Controls:
Factor
Results:
Caffeine
Concentration
/ %
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Heart Rate
Repeat 1 /
bpm
Heart Rate
Repeat 2 /
bpm
Heart Rate
Repeat 3 /
bpm
Heart Rate
Average
bpm
Standard
Deviation
Graph:
Evaluation:
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Ethics
As a class, brainstorm the ethical implications of using animals in
experiments. What precautions did you take to ensure their safety?
Is it appropriate to use animals in scientific research? Does the
Utilitarian argument apply to this practical?
Ethical Point
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Comment
Questions
1 What is the Independent Variable in this experiment?
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Notes, Comments:
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Planning
The quantity of vitamin C in food and drink can be determined
using a simple colour test. Vitamin C decolourises the blue dye
DCPIP (dichlorophenolindolphenol). Vitamin C is an antioxidant
and reduces the DCPIP. DCPIP changes from blue to colourless
(or slightly pink) as it becomes reduced.
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Results:
Test Reagent
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Questions
1 What is an antioxidant?
2 What is a free radical and why are they so bad for DNA?
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Stopclock
Distilled water
Pipette for measuring 2 cm3
Small measuring cylinders
Waterproof marking pen
Method:
1 Cut sections from a single beetroot using a cork borer. Cut
eight 1cm length slices from these sections. Be careful:
beetroot juice stains
2 Place the sections in a beaker of distilled water to wash away
any excess juice
3 Pipette 5cm3 distilled water into three boiling tubes
4 Place a chip of beetroot into each tube and put into a
waterbath at 20C for 30min
5 Remove the beetroot sections and shake the tubes to
disperse the dye in the solution
6 Switch on the colorimeter and calibrate it with a cuvette of
2cm3 of distilled water.
7 Pipette 2cm3 of the dye solution into a clean cuvette and take
an absorbance reading. Repeat for the remaining two chips.
8 Repeat steps 4 to 7 with the other temperatures in the range
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Controls:
Factor
Risk Assessment:
Risk 1:
Precaution:
Risk 2:
Precaution:
Risk 3:
Precaution:
Risk 4:
Precaution:
Results:
Temperature
/ C
Graph:
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Absorbance
1 / AU
Absorbance
2 / AU
Absorbance
3 / AU
Average
Absorbance / AU
Analysis:
Trend
Explanation
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Questions
1 What is the effect of temperature on molecules?
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Says what measurements you will make, how they will be made,
and the level of accuracy that you can expect from your
measurements
Includes an assessment of any risk
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Results:
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Amylase conc / %
Graph:
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Questions
1 Why is iodinated agar used?
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Test tube
2 pipettes (and pipette fillers) or small measuring cylinders
Microscope slides and coverslips
Pair of fine forceps
Mounted needle
Filter paper or soft tissue paper
Microscope with magnifications of 100 and 400
Safety goggles
Method:
1 Put a test tube containing 2 cm3 1 M hydrochloric acid
into a water bath at 60 C.
2 Cut off 12 cm from several root tips of some growing
garlic roots. Choose root tips which are white and have a
firm rounded end; tips that are turning brown will give
poor results.
3 Put the root tips in a watch glass containing
approximately 2 cm3 of acetic alcohol. Heat the watch
glass gently for 5 min using a hot plate.
4 Remove the root tips and place them in a second watch
glass with approximately 5 cm3 ice-cold water. Leave for
45 minutes, then dry the root tips on filter paper. It is
important to blot the tips well to remove the water at
this stage or a precipitate may form when staining
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carefully
for
cells
undergoing
Questions:
1 Identify cells in the following stages of mitosis:
interphase,
prophase,
metaphase,
anaphase
and
telophase. Draw one cell to illustrate each stage. Your
drawings will be simple outlines of the cells and the
groups of chromosomes in them as few other structures
will be visible. Aim to show the relative sizes and positions
of the chromosomes and the cell accurately. Annotate to
describe what is happening.
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Prophase
Metaphase
Anaphase
Telophase
Interphase
Prophase
Metaphase
Anaphase
Telophase Interphase
=
Total number of cells in the field of view
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7 Explain the
practical.
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safety
precautions
taken
during
this
9 You may have found few dividing cells in your root tip(s).
Suggest possible reasons.
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Method:
1 Sprinkle some seeds of white mustard or rapid-cycling
Brassica onto a damp sponge places in a plastic tray. Cover
with transparent cling film and place in a warm, light place to
germinate. When the seedlings have just started to unfold
their cotyledons (seed leaves) they are ready to culture.
2 Measure out 2.5g of agar powder and add to 250cm3 of
distilled water. Boil and stir gently with a glass rod until the
agar dissolves.
3 Whilst the agar is still molten, pour about 2cm depth into
several short-necked test tubes or McCartney bottles. Allow
apex
to solidify.
cotyledons
hypocotyl
sponge
5 Carefully push the cut end of each explant into the agar. Put
one explant into each test tube or bottle. Make sure the
cotyledons do not touch the agar.
6 Cover the tubes with cling film. On each tube or bottle write
your name and the date. Place the tubes in a rack under a
light bank or on a sunny windowsill. Do not open the tubes
again.
7 Observe the progress of your explants daily for ten days and
record when anything of note develops. You will need to pop in
at break / lunch on some days to do this.
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Results:
Day
Observation
1
2
3
4
5
6
7
8
9
10
Questions:
1 What, if anything, would you expect the explants to obtain
from the agar?
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4 Why should you not open the tubes up again once you have set
them up?
6 Explain why the explants can grow and develop new leaves,
stem and roots
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Applications
Flax
Cotton
Hemp
Coir
Jute
Manila
Pulp
Softwood trunks
Paper, cardboard
Fibres can be removed from plant stems by retting. This can be done
by either field retting or water retting.
Field Retting:
Plant stems are cut or pulled up and left in the field to rot; microbial
action breaks down the stalks.
Water Retting:
Stems are cut or pulled up and immersed in water to rot; microbial
action breaks down the stalks. This process produces more uniform,
higher quality fibres, but is more expensive and produces nitrogenrich waste water that must be treated before discharge.
In both kinds of retting, bacteria and fungi breaks down the soft
tissues of the stems leaving the cellulose intact. It is them relatively
easy to remove the cellulose-rich fibres.
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You need to know about the retting process. However, it is timeconsuming and very smelly. Therefore, for these reasons we are
going to do a simpler practical!
Method:
Celery stems
Retort stand & clamp
10g and 50g masses
Results:
Mass Added / g
0
40
80
120
160
200
240
280
320
360
400
440
480
520
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Start length / cm
End length / cm
Extension / cm
Graph:
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Questions
1 What is the structure of cellulose?
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Geramium plants
Cotton wool
Aluminium foil
Standard laboratory equipment
Method:
A series of Geranium plants have been grown in solutions lacking
nitrogen, phosphate, potassium, magnesium, calcium and all
nutrients. A control plant has also been grown for comparison.
Measure the height of the plants and make notes on the appearance
of each plant.
Nutrient
No NO3No PO42No K+
No Mg2+
No Ca2+
No
Nutrients
Control
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Height / cm
Nutrient
No NO3-
No PO42-
No K+
No Mg2+
No Ca2+
No
Nutrients
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Observation
Questions
1 What do plants use Nitrate for?
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Method:
1 Agar plates seeded with E.coli bacteria need to be prepared
2 Using a pestle and mortar crush ~3g of garlic with ~10cm3 of
ethanol.
3 Using a pipette repeatedly dab a sterile paper disc with
ethanol solution from the crushed garlic. After each dab,
wait a few seconds for the ethanol on the disc to evaporate
before repeating. By doing this slowly, you build up garlic on
the filter paper.
4 Repeat steps 2 & 3 for mint
5 Prepare a disc soaked in deionized water and a disc soaked in
dettol. Obtain pre-prepared discs soaked in antibiotic from
the teacher
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Results:
Disc
Dettol
Water
Mint
Garlic
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Graph:
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Questions
1 What is an autoclave?
4 Why is ethanol used as a solvent when crushing the garlic & mint?
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Notes
One way of remembering this is the mnemonic MERC CAR, like this
one
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As you progress through the year try why not fill out the blank
revision cards below? That way you have a complete record of what
you need to learn in the summer before the summer!
Equipment:
Risks:
Controlled factors:
Average:
Range:
Additional Notes:
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none
none
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Equipment:
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