2.1.

Phng php xc nh kh nng c ch peroxidation lipid (th nghim MDA)

c thc hin theo phng php ca Stroev EA, Makarova VG (1998), Jelili A
Badmus et al., (2011) v ca Vin Dc liu - B Y T (2006), c s thay i cho ph
hp vi iu kin ca phng th nghim. Xc nh kh nng c ch peroxy ho lipid
ca mu nghin cu qua vic xc nh hm lng malonyl dialdehyd (MDA), l sn
phm ca qu trnh peroxy ho lipid mng t bo. MDA c kh nng phn ng vi acid
thiobarbituric to thnh phc hp trimethin (c mu hng) c nh hp thu cc i
= 532 nm.
Cch tin hnh:
Tch no chut v nghin ng th trong dung dch m phosphat (pH=7.4)
theo t l 1:10 nhit 0-4oC.
Ly 1ml dch ng th thm vo 0,1 ml mu th cc nng v 0,8ml m
phosphat thm 0.1ml h Penton (FeSO4 0.1 mM : H2O2 15mM theo t l 1:1).
hn hp 37oC trong 15 pht.
Dng phn ng bng 1 ml acid tricloacetic 10%.
Li tm 12000 vng trong 5 pht.
Ly dch trong cho phn ng vi 1 ml acid thiobarbituric 0,8% (theo t l 2:1).
nhit 100oC 15 pht.
Lm lnh v tin hnh o bc sng = 532 nm.
2.2.Tnh ton kt qu
Cng thc tnh phn trm hot tnh chng oxi ho (HTCO)
HTCO (%) = [(ODC ODT)/ODC] 100
ODC : Mt quang hc ca ging chng khng c mu th
ODT : Mt quang hc ca mu th