Product Datasheet

Anti-alpha smooth muscle Actin antibody,

prediluted ab15267
4 Abreviews 7 References 2

Overview

Product name Anti-alpha smooth muscle Actin prediluted

Description to alpha smooth muscle Actin prediluted
Tested applications IHC-Fr, IHC-P
Species reactivity Reacts with: Mouse, Rat, Cat, Human
Predicted to work with: Rabbit, Chicken, Cow, Baboon
Immunogen Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially
sensitive) (Human) (N terminal).
Positive control Smooth muscle or leiomyosarcoma.

Properties

Form Prediluted
Storage instructions Store at +4C. Do not freeze.
Storage buffer Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 50mM Tris, pH 7.6
Purity Immunogen affinity purified
Clonality Polyclonal
Isotype IgG

Applications

Our Abpromise guarantee covers the use of ab15267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
IHC-Fr
IHC-P
Application notes IHC-P: Ready to use for 10 min at RT. No special pretreatment is required for the immunohistochemical staining
of formalin/paraffin tissues.
IHC-Fr: Use at an assay dependent dilution (from PubMed:17149535).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function
Involvement in disease
Sequence similarities
Cellular localization
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously
expressed in all eukaryotic cells.
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are
characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall.
They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim
cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle
cells, and an accumulation of basophilic ground substance.
Belongs to the actin family.
Cytoplasm > cytoskeleton.

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 Product Datasheet

Anti-alpha smooth muscle Actin antibody, prediluted images

ab15267 staining alpha smooth muscle Actin

in human leiomyoma by
Immunohistochemistry (FFPE-sections).

Immunohistochemistry (Formalin/PFA-
fixed paraffin-embedded sections) -
alpha smooth muscle Actin antibody,
prediluted (ab15267)

ab15267 (used X1) staining day 3 wounded

rat skin tissue by IHC-P. The tissue was
formaldehyde fixed and blocked with
peroxidase prior to incubation with the
antibody for 25 minutes. An HRP conuugated
goat polyclonal antibody was used as the
secondary.

Immunohistochemistry (Formalin/PFA-
fixed paraffin-embedded sections) -
alpha smooth muscle Actin antibody,
prediluted (ab15267)
This image is courtesy of an Abreview submitted by Dr
Philip Roby

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Product Datasheet
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