Figure 1: Basic tissue culture plan

Figure 1: Tissue culture laboratory layout

Aseptic Transfer Area

1.Laminarairflowtransferhoodandcomfortablechair
2.Dissectingmicroscope
3.Gasoutlet
4.Vacuumlines
5.Forceps,spatulas,scalpel,anddisposableblades

Media Preparation/Culture Evaluation/Record-Keeping Area

1.Bench
2.Gasoutlet
3.Hotplateandmagneticstirrer
4.Analyticalandtoploadingbalances
5.pHmeter
6.Refrigerator,freezer
7.Waterpurificationandstoragesystem
8.Dishwashingarea
9.Storagefacilitiesglassware,chemicals
10.Autoclave(pressurecookerwillworkforsmallmediavolume)
11.Lowbenchwithinvertedlightanddissectingmicroscopes(avoidlocatingnexttoautoclavesorotherhigh
humidityareas)
12.Fumehood
13.Deskandfilecabinets
14.Desktopcentrifuge,spectrophotometer,microwave(transformationstudiesandprotoplastisolation)