STANDARD OPERATING PROCEDURE FOR THE

USE OF ELECTROPHORESIS EQUIPMENT

Electrophoresis involves the use of high voltages and

carries the risk of electric shock.
Many chemicals commonly used in electrophoresis are
highly toxic.
1. Make sure you have been trained by an experienced
worker in the safe use of electrophoresis equipment.
2. Familiarise yourself with the COSHH forms for the
chemicals you intend to use.
3. Check equipment and wiring before use. Look for
signs of damage. Do not use worn or frayed leads.
4. Use only electrophoresis tanks which have a secure
design preventing contact with buffer when
connected to a power supply.
5. Always disconnect from the power supply before
opening.
6. Switch off power before moving a tank.
7. Clean up spills of electrophoresis buffer or gel mixes
immediately these may contain toxic chemicals e.g.
ethidium bromide or acrylamide.
8. Latex gloves often contain small holes use nitrile
(or other suitable) gloves when immersing hands in
electrophoresis buffers or handling gels.
9. When using vertical electrophoresis equipment, take
care that leakage from the upper buffer chamber
does not cause arcing.
Emergency action in case of electric shock

1. Switch off power at once. Do not attempt to touch the

victim until they have been isolated from the source of
electricity. If you are unable to get to the power supply
you will have to insulate yourself so that you do not
become a casualty. A piece of DRY wood or rolled up
paper could normally be used.
2. Check the casualtys airway and breathing if
unconscious be ready to resuscitate, call for help
immediately
3. Treat any burns when away from the electricity by
flooding the affected part with water for a minimum of
10 minutes. Remove and constrictions if swelling is
likely, keep injury elevated.
Do not burst blisters, apply any creams or sprays or
apply any plasters. Seek help from a first aider.