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Management of chromoblastomycosis: novel perspectives
Philippe Esterrea and Flavio Queiroz-Tellesb
a
International Network of Pasteur Institutes, Institut Pasteur de Guyane, Cayenne
The natural habitat of the fungal species
Cedex, French Guiana and bDepartment of Public Health, Federal University of incriminated
Parana, Curitiba-PR, Brazil Chromoblastomycosis is most frequently observed in
Correspondence to Flavio Queiroz-Telles, Department of Public Health, Federal tropical zones, occurring generally in people engaged
University of Parana, Curitiba-PR, Brazil 82010650
Tel: +41 99721828; fax: +41 33606090; e-mail: queiroz.telles@uol.com.br in forest or bush clearance, but in subtropical regions
such as southern Brazil, it is observed in rural workers,
Current Opinion in Infectious Diseases 2006, 19:148152
especially those who do not routinely wear shoes, leading
2006 Lippincott Williams & Wilkins commonly to lower limb involvement. The various fungal
0951-7375
species identified can be considered as black moulds with
a saprobiotic life in organic matter (rotting wood) and soils
[3,4]. Several reports involve different species of palm
trees harbouring wild strains of Dematiaceae, especially
F. pedrosoi [5]. Observations of typical muriform cells (see
above) in the medulla of cactus-like plants [6] suggest the
importance of these environmental sources. Not surpris-
ingly, the ecology of the pathogenic complexes is clearly
differentiated. In tropical areas, F. pedrosoi is the only
species isolated in the evergreen forests (in the Brazilian
Amazon or in northern Madagascar). In temperate
zones, such as southern Brazil, Uruguay, and northern
148
Copyright Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited.
Chromoblastomycosis Esterre and Queiroz-Telles 149
Copyright Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited.
150 Skin and soft tissue infections
oxidase and transglutaminase enzyme activity observed surgery. Recently, Malagasy doctors used cautery in
at the circulating and tissue levels, is responsible for the conjunction with short-term terbinafine therapy on
filariasis-like lymphoedema observed in the most chronic early-stage lesions of chromoblastomycosis.
cases [14,15]. Microinvasive squamous cell carcinomas,
arising from chronic lesions, have occasionally been Antifungal chemotherapy
described. The azole compounds have both in-vitro and in-vivo
action in several dematiaceous fungi, including chromo-
Antifungal susceptibility testing blastomycosis agents. Their principal mechanism of
At present the standard methods to test the in-vitro action is to block membrane 14-a-demethylase and in-
susceptibility to antifungal drugs are not available for hibit the transformation of lanosterol into ergosterol, a
filamentous and dimorphic fungi. Nevertheless, the vital cell membrane component. Among the azole deriva-
determination of the susceptibility profile, by in-vitro tives, ketoconazole, an imidazolic compound, is not recom-
methods, of clinical isolates of the etiologic agents can mended for chromoblastomycosis because of its hepatic
be used to identify microbiologic resistance but not and endocrine toxicity combined with its lack of efficacy in
to predict clinical response. Some instances of azole F. pedrosoi infections. Saperconazole has been success-
resistance have been observed in sequential isolates fully used in chromoblastomycosis, but it was discontin-
of F. pedrosoi from patients on long-term itraconazole ued in the past decade due to teratogenicity in animal
treatment [16]. The in-vitro mode of action of terbina- models [23]. Fluconazole does not show potent in-vitro
fine is clearly fungicidal and not merely fungistatic as activity against the black fungi. Itraconazole, like fluco-
are most of the other antifungals against filamentous nazole, is a first-generation triazole and is better tolerated
fungi [17]. than ketoconazole. It shows a broad antifungal spectrum
and has been evaluated in several open, noncomparative
Recent therapeutic progress clinical trials. In a series of 30 Brazilian patients treated
Chromoblastomycosis lesions are recalcitrant and ex- with 200400 mg of daily itraconazole, the final assess-
tremely difficult to eradicate. Patients with chromo- ment showed that eight patients (89%) with mild forms of
blastomycosis are a true therapeutic challenge for the disease achieved clinical and mycologic cure after
clinicians. Over the past few decades several treatment 10.9 months of therapy (range 717.6 months). Similar
regimens have been employed [1,8,18,19]. In the early response was noted in 11 (91%) of the 12 patients with
stages, lesions respond to surgical resection, but later, as moderate forms after 12.9 months (range 531 months) of
severity increases, better results are achieved with sys- continuous treatment (Fig. 2). Among the nine patients
temic antifungals. Therapeutic success depends on with severe lesions, four (44%) had clinical and mycologic
the etiologic agent (C. carrionii is more sensitive than response after a mean treatment duration of 30 months
F. pedrosoi), the severity of the disease (oedema and (range 1051 months), and the remaining patients had
dermal fibrosis can reduce the antifungal tissue levels), significant improvement [1]. Based on its pharmacoki-
and obviously, the choice of antifungal drug. As in other netic profile and the data obtained for onychomycosis,
endemic mycoses, comparative clinical trials are lacking pulse treatments have been considered, although never
in chromoblastomycosis. In most of the noncomparative in a large-scale series. Itraconazole is well tolerated even
studies, the lesions are not graded according to severity, in prolonged courses. Conversely, the drug may have
and different authors have used nonstandardized criteria unpredictable gut absorption and irregular plasma levels.
for cure for this mycosis. There is thus no gold standard It is metabolized in the liver via cytochrome P-450, which
therapy for this mycosis but several treatment options may lead to several drug interactions that contraindicate
including systemic antifungals used alone or combined itraconazole for some subjects with chromoblastomycosis
with physical methods such surgery, local heat, or topical [24]. Three new second-generation triazoles were devel-
liquid nitrogen. Recently, topical ajoene was shown to oped in recent years. All these compounds show in-vitro
result in the same response as topic 5-flucytosine in activity against the black fungi. Voriconazole and ravu-
patients with mild forms of chromoblastomycosis caused conazole derived from fluconazole molecule. Voricona-
by C. carrionii [20]. A comprehensive review of the zole is commercially available and differs from
principal therapeutic choices in chromoblastomycosis fluconazole by its broad spectrum and increased bio-
was published by Bonifaz et al. [21]. chemical affinity to fungi 14-a-demethylase. This prop-
erty translates into strong antifungal activity [24]. To
Physical methods date there is no report of the use of voriconazole in
For localized initial lesions, surgery is often a first-line chromoblastomycosis, but it has been successfully em-
solution. To reduce the long course of systemic therapy, ployed in phaeohyphomycosis [25]. Ravuconazole is
various medical teams have proposed combinations of currently under development but has not been tested
drugs with physical methods, the most interesting of in chromoblastomycosis. Posaconazole is an itraconazole
which [22] uses itraconazole in conjunction with cryo- derivative molecule, finishing phase III clinical trials. It
Copyright Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited.
Chromoblastomycosis Esterre and Queiroz-Telles 151
The patient had the disease for 22 years and received itraconazole, 200 mg/day, for 22 months (a). Clinical and mycologic cure was observed at
follow-up (b).
has one of the broadest antimycotic spectrums, being Conclusion: current and future best practices
active even against Zygomycetes. Posaconazole was suc- Ecoepidemiologic studies on chromoblastomycosis show
cessfully used in dematiaceous infections, including that the etiologic agents are ubiquitous in the environ-
phaeohyphomycosis, black grain mycetoma, and chromo- ment where the disease is observed. Opportunities for
blastomycosis caused by F. pedrosoi [26]. infection through different kinds of trauma are frequent,
but conversely the clinical forms are not frequent. Are
Allylamine-based therapy dematiaceous wild strains less adapted to mammalian
Based on its good in-vitro activity (even better than the organisms? Is the inoculum usually so small that the
activity of itraconazole against F. pedrosoi), terbinafine hosts defence system is able to block the progression
therapy at 250500 mg/day has been proposed for this of infection? Is there a genetic susceptibility to the
mycosis. The largest case series (42 patients) has been disease, as suggested by earlier HLA studies [29]?
followed in Madagascar. A global 74.2% cure rate was These questions are unanswered. The experience of
reported at the end of treatment, reaching as high as 81% the Malagasy and South American medical teams leads
after 2-year follow-up [27]. It seems that the fungicidal to the conclusion that therapy combining surgery and
(rather than fungistatic as with the azole derivatives) itraconazole or terbinafine is at present the best protocol,
action of terbinafine on the early ergosterol synthesis especially in cases of extensive disease. The new second-
step is a clear advantage; drug interaction level is also generation triazoles, especially posaconazole, may play a
lower [19]. This explains why some patients have been role in the treatment of chromoblastomycosis.
treated successfully using a 1 g/day dosage without pre-
senting with an increased rate of adverse events. Inter- The next step, developing a genome-wide expression
estingly, and surprisingly, it was found in vivo that profile in response to antifungal drugs and based on the
terbinafine showed a significant antifibrotic effect (on use of real-time polymerase chain reaction and micro-
newly synthesized, poorly cross-linked extracellular array, is being quickly implemented in mycology [30]. In
matrix), a finding that, unfortunately, has not been addition to these molecular tools, the availability of
studied at the molecular level [14]. modern medical imaging and combined azoleallylamine
therapy is modifying the medical management of this
Combined therapy tropical mycosis [17,31,32].
In the past, itraconazole has been combined with
5-flucytosine with some success [28]. The recent in-vivo
demonstration of a synergistic effect between itracona- References and recommended reading
Papers of particular interest, published within the annual period of review, have
zole and terbinafine on chronic resistant chromoblas- been highlighted as:
tomycosis is an interesting perspective [18]. As for of special interest
of outstanding interest
antimicrobial and antimalarial therapies, the fact that Additional references related to this topic can also be found in the Current
the mechanism of action of the two combined molecules World Literature section in this issue (pp. 194195).
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Copyright Lippincott Williams & Wilkins. Unauthorized reproduction of this article is prohibited.
152 Skin and soft tissue infections
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