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BMB7010 Exam Answers Dec07
BMB7010 Exam Answers Dec07
d. The eukaryotic transcription factor that binds to the TFIID (or TBP okay)
TATA box.
(16 points)
Lk = Tw + Wr
3. Indicate which of the following apply to prokaryotic and eukaryotic protein synthesis.
Check one or both or no boxes.
Prokaryotic Eukaryotic
normal 5 C C T A T T G G T C T A T T T T C C A C C C T T A G G C T G C T G
exon
mutant 5 C C T A T T A G T C T A T T T T C C A C C C T T A G G C T G C T G
Name ____________________
BMB7010 Biochemistry December 13, 2007
Fourth Exam (Evans) Page 3 of 5
7. The E. coli DNA polymerase holoenzyme is shown below. Identify the function,
given in the list below, of each of the following components and write the corresponding
letter in the space.
DnaB
I
2 J
2 F
DnaB G
a. The activated intermediate in the reaction that attaches the aminoacyl adenylate
amino acid to the tRNA or AA-AMP
editing or
b. The site on the aminoacyl tRNA synthetase that removes an
proofreading site
amino acid that has been incorrectly attached to tRNA.
CCA 3 (acceptor
c. The attachment site of the amino acid on tRNA. stem okay)
e. The tRNA loop that base pairs with the codon on mRNA. anticodon loop
(5 points)
A or H
d. binds to acetylated histones.
10. Compare the regulation of the lac and pur operons in E. coli. Check the appropriate
box or boxes (6 points) .
lac operon pur operon
11. Identify the following involved in the synthesis of a integral membrane protein.
12. Identify the following components in the transcription bubble shown in the diagram
and write the corresponding letter in the space.
a. nascent RNA C B
A
b. DNA coding strand B
A D
c. DNA template strand E
E
d. RNA DNA hydrid
C
D F
e. elongation site
F
f. direction polymerase moves G
(12 points)