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Optimizing Scale-Up PDF
Optimizing Scale-Up PDF
Optimizing scale-up chromosome. This is often the case for Pichia pastoris
constructions but gene integration can reduce the
expression level [9].
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104 Opinion TRENDS in Biotechnology Vol.20 No.3 March 2002
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Opinion TRENDS in Biotechnology Vol.20 No.3 March 2002 105
process should be performed using a two tiered For example, adjunction of a cofactor or substrate
approach. First the well-known avenues described in can stabilize an enzyme and increase the final
this article and others allow a rational and scientific productivity. In this area of research, the imagination
optimization. In a second approach the study of the and creativity of the scientist can achieve outstanding
property of the expressed protein should suggest results that are well out of reach of the best
more unusual parameters to be assayed. multifactorial experiment plan.
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issues in the clinical said than done. The clinical cycle is a long,
complicated process that has been developed over a
long period. The clinical process involves several
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