LESSON PLAN

July October 2016 Branch: BT

Year : IV
Sub Code : BT 6711
Semester : VII
Sub Name : Downstream Processing Lab
L 0 T 0 P 4 C 2 Date: 07.07.2016
Staff In-Charge : Dr. Ansel Vishal L No of Students: 29
Designation/ Department : Biotechnology

Objective:
To provide hands on training in Downstream Processing through simple experimentations in the
laboratory. This will be a pre-requisite for project work.
To practice the students
o To understand the nature of the end product, its concentration, stability and degree of
purification required.
o To design processes for the recovery and subsequent purification of target biological
products.

Date Teaching
No. of No. of
Books method PEOs SOs
S.No Topics Pages Hours Hours
Referred Satisfied Satisfied
Proposed Actual Required Taken

Introduction to the
08.07.20 Laboratory & Precipitation
1 DEMO
16 - Ammonium Sulphate
Precipitation
15.07.20 Aqueous Two Phase
2 DEMO
16 Extraction of Biologicals
22.07.20 Solid Liquid Separation -
3 DEMO
16 Centrifugation
High Resolution
29.07.20
4 Chromatography - Affinity DEMO
16
Chromatography
High Resolution
05.08.20 Chromatography - Ion
5 DEMO
16 Exchange
Chromatography
Precipitation - Caesin by
12.08.20
6 Isoelectric Precipitation DEMO
16
Method
19.08.20
7 Ultrafiltration Separation DEMO
16
Cell Disruption
26.08.20 Techniques -
8 DEMO
16 Ultrasonication, French
Press
02.09.20 Product Polishing - Spray
9 DEMO
16 Drying , Freeze Drying
High Resolution
09.09.20
10 Chromatography - Gel DEMO
16
Filtration
Total Number of Hours Planned
Course Delivery Plan:

1 2 3 4 5 6 7 8 9 10 11 12 13
Weeks

Units V
I II III IV

REFERENCES:

S. No Book Title Author(s) Edition / Publishers

P.A. Belter, E.L.

Bioseparations Downstream Wiley Interscience
1. Processing For Biotechnology
Cussler And Wei-
Pun. (1988
Houhu

Product Recovery In Bioprocess

Butterworth-
2. Technology Biotechnology By Open R.O. Jenkins,
Heinemann (1992).
Learning Series

Purification Principles, High J.C. Janson And L. Protein VCH Pub.

3. Resolution Methods And Applications, Ryden 1989

Prepared by Approved by

Signature

Name Dr. Ansel Vishal L Dr. Mathan Periasamy.C Dr.V. R.Sampath

Designation Assistant Professor HOD Principal