INSTRUCTION MANUAL

MODEL L-2450 DIODE ARRAY DETECTOR

Please read through this manual carefully

and store it in a safe place.

Before using the instrument, read the safety

instructions and precautions carefully.
Keep this manual in a safe place nearby so it
can be referred to whenever needed.
 INSTRUCTION MANUAL
FOR
MODEL L-2450 DIODE ARRAY DETECTOR

Hitachi High-Technologies Corporation

3rd Edition, September 2003

1st Edition, 2003
Copyright Hitachi High-Technologies Corporation 2003. All rights reserved. Printed in Japan. Part No. 890-9451-2 TM-K (HTT-LT)
PREFACE
We thank you for purchasing the Hitachi Model L-2450 Diode
Array Detector (hereinafter referred to as DAD).
The L-2450 DAD has been specifically designed for detecting the
change in absorbance in combination with a liquid
chromatograph.
This instrument is intended for use by persons having a basic
knowledge of chemical analysis procedures. Keep in mind that
improper use of analytical instruments, chemicals or samples
would result not only in wrong analytical data but also in
consequences adverse to safety.
Please read this instruction manual carefully before attempting
operation and acquaint yourself with the DAD to enable its
correct use.
After reading through this manual, keep it handy so it can be
referred to whenever needed.

ABOUT THIS MANUAL

This instruction manual has been prepared for the user of the
L-2450 DAD, and describes the operating procedures and
maintenance/checkup instructions for the instrument.
First of all, please read IMPORTANT and SAFETY
SUMMARY at the beginning of this manual to ensure safety in
operation of the instrument.
If installation of the instrument is not yet completed, refer to
Section 2.
When operating the instrument for the first time, read through
Sections 3 and 4 to gain a full understanding of its structure and
basic operation.

-1-
-2-
IMPORTANT
PRECAUTIONS ON CE CONFORMITY MARKING

1. Electromagnetic Compatibility Requirement

This equipment conforms to the EC Directive 89/336/EEC

and has the CE conformity marking. It is designed to
satisfy the European Norm EN61326 (1997) and its
amendment 1 (1998). This equipment is classified as
Class A of EN61326. So, this equipment must not be
used in domestic establishments nor in establishments
directly connected to a low voltage power supply network
which supplies buildings used for domestic purpose.
And this equipment is also designed to comply with table 1
Minimum immunity test requirements and with evaluation
table 2 Continuous unmonitored operation. If the
equipment is used near an intense electromagnetic source,
however, interfering noise may be given to the instrument
to cause an adverse effect on its performance or
functionality.

2. Safety Requirement

This equipment also conforms to the EC Directive

73/23/EEC and has the CE conformity marking. It is
designed to satisfy the European Norms EN61010-1: 1993
and Am-1 & 2: 1995.
This instrument is classified in class 1, pollution degree 2 of
EN61010-1.

-3-
2. Possible Electromagnetic Wave Interference Affecting
This Instrument

If this instrument is used near an intense electromagnetic

source, interference noise may be given to the instrument
to incur an adverse effect on its performance or
functionality.
To prevent this, use the specified system connection cables
in strict accordance with the instruction manual.
The instrument is designed to minimize possible
electromagnetic wave interference affecting it if the
specified cables are connected properly.
However, there is no guarantee that electromagnetic wave
interference will not occur in this instrument. If the
instrument does incur electromagnetic wave interference,
which can be determined by turning on and off possible
sources of electromagnetic wave interference nearby, the
user is encouraged to try to correct the interference by one
or more of the following measures:

Reorient the instrument.

Increase separation between the instrument and
possible sources of electromagnetic wave interference.
Connect the instrument to an outlet on a circuit different
from that to which possible sources of electromagnetic
wave interference are connected.
Confirm that any other device connected with the
instrument is not affected by electromagnetic wave
interference.

-4-
WARRANTY ON PRODUCT
The L-2450 DAD is warranted to operate according to the
specifications given in the instruction manual, provided it is used
in accordance with the instructions described in the manual.
We make no warranties, either express or implied, as to
marketability/merchantability and fitness for a particular purpose.
No oral or written information or advice given by our dealers,
distributors, agents or their employees without our express
permission shall create a warranty or in any way increase the
scope of this warranty.

(1) Scope of Warranty

Any parts which prove to be defective in design or

workmanship during the warranty period will be repaired
without charge.
A substitute part may be used for repair, or replacement
with an equivalent product may be made instead of repair.
Such system components as a personal computer and
printer to be updated frequently for improvement may not
be available in original versions at the time of replacement.
Note that this warranty does not apply to the instrument if
discarded or resold without our, consumables parts and
lifetime-expired parts.
No reliability is assumed for damage to data or application
software due to any possible fault or failure of the
instrument.

(2) Warranty Period

One year from the date of initial installation

(3) Limitations and Exclusions on Warranty

Note that this warranty is void in the following cases, even if

they occur within the warranty period.

(a) Failure due to operation at a place not meeting the

installation requirements specified by us

(b) Failure due to power supply voltage/frequency other

than specified by us or due to abnormality in power
supply

-5-
 (c) Corrosion or deterioration of the tubing due to
impurities contained in gas, air or cooling water
supplied by the user

(d) Corrosion of the electric circuits or deterioration of the

optical elements due to highly corrosive atmospheric
gas

(e) Failure due to use of hardware, software or spare parts

other than supplied by us

(f) Failure due to any operation other than described in

this instruction manual or repair not approved by the
manufacturer

(g) Failure due to maintenance or repair by a service

agent not approved or authorized by us

(h) Failure due to relocation or transport without our

approval after initial installation

(i) Failure due to disassembly, modification or relocation

not approved by us

(j) Failure due to acts of God, including fire, earthquake,

storm, lightning, social disturbance, riot, crime,
insurrection, war (declared or undeclared), radioactive
pollution, contamination with harmful substances, etc.

(n) Failure of the hardware due to computer virus infection

(4) Warranty Card

The warranty card will not be reissued. Keep it in a safe

place.

-6-
INSTALLATION, RELOCATION AND
AFTER-SALE TECHNICAL SERVICE
(1) Installation and Relocation

(a) Installation at delivery shall not be carried out by the

user. It shall be carried out by our service office or
the engineers who have been trained and qualified for
this purpose by us in order to use the instrument safely
and accurately.

(b) Before installation, the user shall make preparations

for satisfying the installation requirements in
accordance with this instruction manual.

(c) When relocation becomes necessary after initial

installation (delivery), please contact the dealer from
whom you purchased it or our service office.

(2) After-sales Service

(a) For after-sales service, contact our sales

representative or service office.

(b) For service after the warranty period, consult us with

regard to a maintenance and inspection service
contract.

-7-
TECHNICAL SEMINARS AND TRAINING COURSES FOR
USERS
We offer technical seminars and training courses at either our or
users facilities to ensure proper and safe operation of the
analytical instrument to its full performance. For further
information, contact our sales representative. (Applicants will
be charged.)

CAUTION ON DISPOSAL OF INSTRUMENT

Although at present the instrument does not use materials that
would directly cause environmental disruption, the environmental
protection laws and regulations may be revised or amended.
When planning to dispose of the instrument, therefore, be sure to
check the latest issues of the relevant laws and regulations or
consult our service office.

-8-
OTHER PRECAUTIONS
1. Handling of Chemicals and Samples

(1) The user is responsible for following relevant legal

standards and regulations in handling, storage and disposal
of chemicals and samples used in analytical operations with
this instrument.

(2) Reagents, standard solutions and accuracy-control

samples shall be handled, stored and discarded as
instructed by the respective suppliers.

2. Notice on This Instruction Manual

(1) Information contained in this manual is subjected to change

without notice for product improvement.

(2) This manual is copyrighted by Hitachi High-Technologies

Corporation with all rights reserved.

(3) No part of this manual may be reproduced or transmitted in

any form or by any means without the express written
permission of Hitachi High-Technologies Corporation.

-9-
 SAFETY SUMMARY
Before using the Hitachi Model L-2450 DAD, be sure to read the
following safety instructions carefully.

General Safety Guidelines

Follow all the operating procedures provided in the instruction

manual for each instrument.
Installation at delivery shall be carried out by the engineers
who have been trained and qualified for this purpose by us.
It shall not be carried out by the user.
Be sure to observe the warnings indicated on the instrument
and in the instruction manual. Failure to do so could result in
personal injury or damage to the instrument.

The hazard warnings which appear on the warning labels on the

product or in the manual have one of the following alert headings
consisting of an safety alert symbol and signal word DANGER,
WARNING or CAUTION.

DANGER : Indicates an imminently hazardous

situation which, if not avoided, will result
in death or serious injury.
WARNING : Indicates a potentially hazardous
situation which, if not avoided, can result
in death or serious injury.
CAUTION : Indicates a hazardous situation which, if
not avoided, can result in minor or
moderate injury.
: Safety alert symbol used for calling
attention to a potential hazard which
could cause personal injury.
To avoid possible injury or death,
observe all the safety messages
following this symbol.
NOTICE : Indicates a hazardous situation which, if
avoided, can result in damage to
property.

SAFETY - 1
 SAFETY SUMMARY
General Safety Guidelines (Continued)

In addition to the above, the following signal word is used to

indicate instructions for ensuring proper use of the product.

NOTE: Indicates an instruction for ensuring correct use of

the product and accurate analysis therewith.

SAFETY - 2
 SAFETY SUMMARY
General Safety Guidelines (Continued)

Precautions on Use

Before using this instrument, be sure to read the instruction

manual carefully to acquire a full understanding of its
functions and operations.

Keep the instruction manual in a safe place nearby so that it

can be referred to whenever needed.

Be sure to follow the operating procedures and usage

described in the instruction manual.

Be sure to understand and follow all the safety instructions

and precautions given in this manual.

Observe all of the above instructions. Failure to do so could

result not only in wrong analytical data or but also in personal
injury.

Do not modify the instrument, use non-specified parts, nor

remove safety devices, as it could be hazardous.

When using a chemical for analytical operation, be sure to

provide proper ventilation in the laboratory room as per local
requirements. Inadequate ventilation could endanger human
health.

SAFETY - 3
 SAFETY SUMMARY
General Safety Guidelines (Continued)

Precautions on Installation, Maintenance and Relocation

Installation at delivery shall not be carried out by the user.

It shall be carried out by our service office or the engineers
who have been trained and qualified for this purpose by us in
order to use the instrument safely and accurately.

At installation, check if any standard accessory is missing.

If missing or damaged, or if you have any question, then
contact your nearest sales representative.
If the instrument is operated without any standard accessory
equipped, it may malfunction to cause a hazardous condition.
In such a case, follow the instructions given by the installation
engineer.

Do not modify the instrument, replace parts that are not user-
serviceable, use non-specified parts, nor remove safety
devices, as it could be hazardous.

Installation at delivery, maintenance and relocation should be

referred to service personnel qualified by us.

Do not perform any operation or action other than described

in the instruction manual. When in doubt, please contact the
dealer from whom you purchased the instrument or our
service office.

Keep in mind that the hazard warnings in the instruction

manual or on the instrument cannot cover every possible case,
as it is impossible to predict and evaluate circumstances
beforehand. Be alert and use your common sense.

SAFETY - 4
 SAFETY SUMMARY
General Safety Guidelines (Continued)

Maintenance to be performed by the user should be within the

scope described in this instruction manual.
Before proceeding to maintenance, be sure to attain a clear
understanding of maintenance items described in the
instruction manual. Do not carry out any maintenance item
other than described in the instruction manual. Failure to do
so may cause trouble in the instrument or personal injury.

Avoid relocating the instrument after installation.

The precisely adjusted mechanical system may be adversely
affected by possible vibration during relocation.

If any warning label is deteriorated due to long use or

damaged, contact our service office.

SAFETY - 5
 SAFETY SUMMARY
WARNING: Ignition of Flammable Chemicals!
Exercise utmost care when handling flammable chemicals.

Beware of ignition hazard when using flammable chemicals

such as organic solvents.

Always check the flow path for the following points. If an

abnormality is found, stop operation immediately.

Leakage of solvent used or waste solution

Leakage of solvent inside the instrument

Well-ventilate the laboratory room where the instrument is

used.

This instrument is not of an explosion-proof type. Do not use

organic solvents having an ignition point below 70 C in
unattended operation.

When using flammable chemicals, be careful about possible

ignition due to static electricity. Particularly when using non-
conductive chemicals, employ a conductive vessel and
provide a correct grounding connection.

WARNING: Explosion of Vapor from Flammable

Chemicals!
Exercise utmost care when handling flammable chemicals.

If a flammable chemical such as organic solvent leaks from

the flow path of the instrument and its vapor concentration
exceeds the explosion limit, it may cause spontaneous
combustion with dangerously explosive results.

When using a flammable and readily volatile chemical, be

sure to check for leakage from the flow path of the instrument
and ventilate the laboratory room adequately.

SAFETY - 6
 SAFETY SUMMARY
WARNING: Electric Shock due to Contact with Inside
of Instrument!
Beware of electric shock. Potentially dangerous voltages are present within
the instrument.

Before removing the instrument cover for replacement of parts or

any other work, be sure to turn off the power switch and unplug
the power cord.

WARNING: Electric Shock due to Improper

Grounding!
Ground properly to prevent an electric shock hazard.

Be sure to use the power cable supplied with the instrument.

Use of a different power cable may result in an electric shock
hazard.

This instrument is of a plug-connected type specified in

European Norm EN61010-1. So connect the power cable to
a grounded three-wire outlet.

When a grounded three-wired outlet is not available, the

power cable may be connected to a two-wire outlet through a
conversion adapter. In this case, be sure to provide a proper
grounding connection.

SAFETY - 7
 SAFETY SUMMARY
CAUTION: Burns due to Contact with Hot Part!
Exercise utmost care when replacing the light source lamp!

The lamp and lamp cover are hot during use or immediately
after use. Touching the hot lamp cover or lamp could result
in burns.

Before lamp replacement, turn off the power switch and wait
for 30 minutes until the lamp cools down sufficiently.

SAFETY - 8
 SAFETY SUMMARY
NOTICES

Precautions on Use of Reagents

Fluorocarbon resin and quartz materials are used for the wetted
parts of the instrument. Never use solvents that would corrode
these materials.

Precautions on Use of Corrosive Solvents

The drain path for carrying leakage solutions is made of

polypropylene.

The materials inside the instrument are susceptible to

corrosion by strong acid, strong alkali and organic solvents.

When using corrosive solvents, make sure that the tubing

connections are not loose.

Using the pump pressure limiter function or by some other

means, make setting so that liquid delivery is stopped
automatically if leakage occurs.

Precautions on Disposal of Waste Solution

Be sure to collect waste solution and treat it properly for disposal

according to the relevant laws and regulations.
Improper disposal treatment of waste solution may result in
environmental pollution, and could also lead to a penalty.

Precaution on Accuracy/Precision of Measured Values

Make control sample measurement to ensure that the

performance of the instrument remains normal.

SAFETY - 9
 SAFETY SUMMARY
Precaution on High Pressure

In the liquid chromatograph, high pressure is applied to its flow

path during operation. Before attempting operation, make sure
that there is no leakage from the flow path. And when using a
harmful solvent, be sure to wear safety goggles.

Prevention of Instrument Tipover

Provide a proper means for preventing the instrument from

tipping over accidentally.

Precaution on Waste Solution Bottle

Prepare a waste solution bottle having a sufficient capacity

(sufficient for the total quantity of mobile phase at least).

SAFETY - 10
 SAFETY SUMMARY
WARNING LABELS

The warning labels shown below are attached to the

L-2450 DAD.
Read the warning labels carefully and check the instructions on
them to acquire a clear understanding with reference to actual
parts.
Periodically check if these warning labels are clean to allow easy
reading over a safe distance.
If any one of them becomes illegible due to deterioration, contact
our service office for replacement with a new one.

(1) Explosion of Vapor from Flammable Chemicals

WARNING
Attached to front
of left side
Beware of explosion!
Can cause serious injury.
High concentration of
organic solvent vapor may
lead to explosion.

Do not allow flammable

chemicals to leak.

810-1952

(2) Ignition of Flammable Chemicals

WARNING Attached to front

of left side
Beware of ignition!
Can cause death or serious
injury.

Refer to manual
when using flammable
chemicals.

810-1951

SAFETY - 11
SAFETY SUMMARY
(3) Electric Shock due to Contact with Power Supply to Light
Source

WARNING
Attached to front
Beware of high voltage : 500V. right
Can cause fatal or
serious injury.

Before removing light source

cover, turn power switch off.
Never release safety mechanism
inadvertently.

810-1958

(4) Burns due to Contact with Hot Part

CAUTION
Attached to front
Beware of high temperature! right
Can cause burns.

Light source lamp and lamp

chamber remain hot for a while
even after power-off
Before lamp replacement, turn
power off and wait until it
becomes sufficiently cool.

810-1962

SAFETY - 12
 CONTENTS

PREFACE ............................................................................................................................ 1

ABOUT THIS MANUAL ....................................................................................................... 1

IMPORTANT ........................................................................................................................ 3
PRECAUTIONS ON ELECTROMAGNETIC WAVE
INTERFERENCE ..................................................................... 3
WARRANTY ON PRODUCT ................................................... 5
INSTALLATION, RELOCATION AND
AFTER-SALE TECHNICAL SERVICE..................................... 7
TECHNICAL SEMINARS AND
TRAINING COURSES FOR USERS ....................................... 8
CAUTION ON DISPOSAL OF INSTRUMENT ......................... 8
OTHER PRECAUTIONS.......................................................... 9

SAFETY SUMMARY......................................................................................SAFETY-1
General Safety Guidelines...................................SAFETY-1
Precautions on Use...................................................SAFETY-3
WARNING: Ignition of Flammable Chemicals!....SAFETY-6
WARNING: Explosion of Vapor from
Flammable Chemicals! ....................SAFETY-6
WARNING: Electric Shock due to Contact with
Inside of Instrument! ........................SAFETY-7
WARNING: Electric Shock due to
Improper Grounding! ........................SAFETY-7
CAUTION: Burns due to Contact with
Hot Part! ...........................................SAFETY-8
NOTICES ..................................................................SAFETY-9
WARNING LABELS ..................................................SAFETY-11

1. OUTLINE................................................................................................................... 1-1
1.1 Isocratic System...................................................... 1-1
1.2 Low-pressure Gradient System ............................. 1-3
1.3 High-pressure Gradient System ............................ 1-5

2. INSTALLATION ........................................................................................................ 2-1

2.1 Unpacking ................................................................ 2-1
2.2 Installation Conditions............................................ 2-2
2.3 Installation Space.................................................... 2-3
2.4 Installation Environment ........................................ 2-4
2.5 Check of Delivered Contents ................................. 2-5

-i-
 2.6 Assembly ................................................................. 2-6
2.6.1 System Power Supply ................................. 2-6
2.6.2 System Wiring ............................................. 2-9
2.6.3 System Piping ............................................. 2-10
2.6.4 Instrument-to-instrument Fastening ............ 2-14
2.6.5 Removal of Transport Screw
and Sponge................................................. 2-15

3. FUNCTION ................................................................................................................ 3-1

3.1 Front Panel............................................................... 3-1
3.2 Rear Panel................................................................ 3-2
3.3 Operating Principle ................................................. 3-3
3.4 Specifications .......................................................... 3-4

4. OPERATION ............................................................................................................. 4-1

4.1 Turning On Power ................................................... 4-1
4.2 Cautions on Operation............................................ 4-2
4.3 Shutdown Procedure .............................................. 4-3
4.4 Washing after Use ................................................... 4-3

5. MAINTENANCE AND INSPECTION ........................................................................ 5-1

5.1 Performance Check at Installation ........................ 5-1
5.1.1 Operation Check ......................................... 5-1
5.1.2 Performance Check .................................... 5-1
5.2 Periodical Inspection .............................................. 5-8
5.3 Maintenance/Inspection Procedure ....................... 5-9
5.3.1 Check and Washing of Flow Cell ................ 5-9
5.3.2 Disassembly and Washing of Flow Cell...... 5-15
5.4 Troubleshooting ...................................................... 5-19
5.5 Error Messages ....................................................... 5-20

6. REPLACEMENT PARTS .......................................................................................... 6-1

6.1 Replacement Parts and Consumables .................. 6-1
6.2 Replacement of Lamp ............................................. 6-2
6.2.1 Replacement of D2 Lamp ............................ 6-3
6.2.2 Replacement of W Lamp ............................ 6-9
6.2.3 Replacement of Hg Lamp ........................... 6-13

APPENDIX 1. DESCRIPTION OF CONTACT SIGNAL

COMMUNICATION..................................................................APPENDIX-1

APPENDIX 2. TERMINOLOGY ......................................................................APPENDIX-7

- ii -
 1.1

1. OUTLINE

The liquid chromatograph system consists of a pump for

feeding an eluent, a sample introducing section (autosampler
or manual injector) for injecting a sample, a column for
chromatographic separation of the injected sample, a column
oven for maintaining the column at a constant temperature
and a detector section for detecting each separated
component of the sample as a signal. If necessary, a
degasser for deaerating an eluent and/or a gradient device for
varying the mixing ratio of eluents with time can also be
incorporated in the system.

1.1 Isocratic System

This is a simple system in which analysis is made with a single

solvent.

Washing solution Eluent

Organizer

L-2450 DAD

L-2300 Column
Oven
Column

L-2200
Autosampler

L-2100/2130
Pump

Degasser Drain

1-1
1.1 Isocratic System

(1) An eluent is fed by the pump through the degasser.

(2) A sample is injected by the autosampler.

(3) The injected sampler is separated through the column,

whose temperature is kept constant by the column oven.

(4) Each separated component of the sample is detected as

a signal by the detector.

1-2
 1.2

1.2 Low-pressure Gradient System

In the low-pressure gradient system, two or more solvents are

mixed in the low-pressure section and then fed by a single
pump. The mixing ratio is made to vary with time in
chromatographic separation.

Washing solution Eluent

Organizer

L-2450 DAD

L-2300 Column
Column
Oven

L-2200
Autosampler

Mixer

L-2100/2130
Pump
Degasser

Drain
Low-pressure
gradient unit

(1) Each eluent is aspirated from the degasser into the low-
pressure gradient unit.

(2) In the low-pressure gradient unit, the eluents are mixed

at the preset mixing ratio.

(3) The mixed eluents are further mixed by the mixer and
then fed to the next section.

(4) A sample is injected by the autosampler.

1-3
1.2 Low-pressure Gradient System

(5) The injected sample is separated through the column,

whose temperature is kept constant by the column oven.

(6) Each separated component of the sample is detected as

a signal by the detector.

1-4
 1.3

1.3 High-pressure Gradient System

In the high-pressure gradient system, two or more solvents

are fed by multiple pumps and mixed in the high-pressure
section for liquid delivery.

Washing solution Eluent

L-2450 DAD

Organizer L-2300 Column

Oven
Column

L-2100/2130
Pump
L-2200
Autosampler

Degasser Mixer Drain

(1) From the degasser, eluents are aspirated into each pump.

(2) The eluents are fed from the pump at a flow rate
corresponding to the preset mixing ratio.

(3) The eluents thus fed are mixed at the mixer and then fed
to the next section.

(4) A sample is injected by the autosampler.

(5) The injected sample is separated through the column,

whose temperature is kept constant by the column oven.

(6) Each separated component of the sample is detected as

a signal by the detector.

1-5
 2.1

2. INSTALLATION

2.1 Unpacking

Unpack the shipping crate carefully while avoiding shocks to it,

take out the main unit and standard accessories, and place
them on a sturdy and level desk or bench.

NOTE: Installation at delivery shall not be carried out by the

user. It shall be carried out by our service office or
the engineers who have been trained and qualified
for this purpose by us in order to use the instrument
safely and accurately.
Before installation, the user shall make preparations
for satisfying the installation requirements in
accordance with this instruction manual.
When relocation of the instrument becomes
necessary after initial installation (delivery), please
notify the dealer from whom you purchased it or our
service office.

2-1
2.2 Installation Conditions

2.2 Installation Conditions

Confirm the following requirements before installing the

instrument.

(1) Power Supply

Line voltage : 100 to 240 V AC

Fluctuation should be within 10% of
the rated voltage.

Frequency : 50 or 60 Hz
Fluctuation should be within 0.5 Hz of
the rated frequency.

Power capacity : 200 VA or more for L-2450 DAD alone

500 VA or more for total system
Provide power supply sufficient for
combinational use with other devices.

Connection : Socket connection

Power supply shall be available near
the installation place for the system
including this instrument.
Grounded three-wire outlet (4 places)

WARNING
Ground Properly to Prevent Electric Shock Hazard!

Be sure to use the power cord supplied with the

instrument. Use of a different power cable may result
in an electric shock hazard.
This instrument is of a plug-connected type specified
in European Norm EN61010-1. So connect the power
cable to a grounded three-wire outlet.
When a grounded three-wire outlet is not available, the
power cable may be connected to a two-wire outlet
through a conversion adapter. In this case, be sure to
provide a proper grounding connection.

2-2
 2.3

(2) Ground Terminal

It is required to provide a grounding connection having a

resistance of 100 or less within a distance of 3 m from
the instrument.

Table tap with

ground cable
Power outlet

AC adapter L-2450
DAD
Grounding
resistance: Ground
750 mm or more 100 or less terminal
underground

Ground wire
(green)
Connector
Copper plate

300 mm Underground

2 mm
10 mm

Fig. 2-1 Grounding Connection

2.3 Installation Space

For installation of the L-2000 series unit, it is required to

provide a space of 640 mm (W) 550 mm (D) or more.
Also provide a space of at least 100 mm at the rear and both
sides of the unit.
Prepare a level and sturdy desk or bench capable of
withstanding a weight of 150 kg or more.

2-3
2.4 Installation Environment

2.4 Installation Environment

When selecting an installation place for the instrument, be

sure to check the following conditions.

(1) Operating Temperature

15 to 35 C
Temperature variation during measurement should be
minimized. Take utmost care not to allow condensation.

(2) Operating Humidity

45 to 85%

(3) Atmosphere

(a) Well ventilated.

(b) Free from acidic or alkaline gases that could corrode

metals significantly.

(c) Free from organic solvent gases (particularly

benzene and thinner) that could affect the paint
coating on the instrument .

(4) Other Requirements

(a) Not exposed to direct sunlight.

Exposure to direct sunlight could result in
deterioration in performance or discoloration of the
instrument. Avoid installing the instrument near a
window of the laboratory room.

(b) Not exposed to drafts directly.

(c) Free from noticeable vibration or shock.

(d) Apart from a heat source such as a gas burner,

electric heater or oven.

(e) Apart from a source of strong magnetic field such as

an electric welder, high-frequency electric furnace or
pole transformer.

2-4
 2.5

(f) Free from excessive dust.

Contamination with dust could deteriorate the
performance of the instrument.

(g) No abrupt fluctuation in line voltage.

Fluctuation in line voltage could cause noise in
analytical data.

(h) Avoid frequent ON/OFF operations of an electric

device not equipped with a noise suppressor circuit
(stirrer, vibrator, etc.) on the same power line as for
this instrument.

2.5 Check of Delivered Contents

After completion of unpacking, check the delivered contents

against the packing list supplied with the instrument. If any
part is missing or damaged, or if you have any question,
contact our sales representative or service office.

2-5
2.6 Assembly

2.6 Assembly

2.6.1 System Power An organizer is available for the L-2000 series system.
Supply Connectable to the organizer (item to be prepared separately)
are two pumps, one autosampler and one detector.
The column over has its own power cord. In a situation
where two detectors are provided with a single organizer
installed, use a proper AC adapter (option). The power cable
from the organizer and AC adapter power cable should be
connected to the power connector at the left rear side of the
instrument.

Power connector

2-6
 2.6.1

(1) Isocratic System/Low-pressure Gradient System

Organizer

Square connector
cable
L-2450 DAD

L-2300 Column
Oven

L-2200
Autosampler Round connector
cable

L-2100/2130
Pump

To AC power
source

Connect the respective power cables of the organizer

and column oven to the AC power source. For the
L-2100/2130 Pump and L-2200 Autosampler, connect the
round connector cables from the organizer to their power
connectors. For the L-2450 DAD, connect the square
connector cable from the organizer to its power
connector.

2-7
2.6 Assembly

(2) High-pressure Gradient System

Square connector
cable

L-2450 DAD

Organizer
L-2300 Column
Oven

Round connector
cable

L-2200
Autosampler L-2100/2130
Pump

To AC power
source

Connect the respective power cables of the organizer

and column oven to the AC power source. For the
L-2100/2130 Pump and L-2200 Autosampler, connect the
round connector cables from the organizer to their power
connectors. For the L-2450 DAD, the square connector
cable from the organizer to its power connector.

2-8
 2.6.2

2.6.2 System Wiring In the L-2000 series, connect the e-Line cable to the e-Line
connector equipped on the left rear of the instrument.
Connect the D-line cable between the synchronous serial
communication connector of the L-2450 DAD and the USB
interface board.

e-Line connector Synchronous serial

communication
connector

(1) Isocratic/Low-pressure Gradient System

Organizer

L-2450 DAD

e-Line cable
L-2300 Column
Oven

e-Line cable
L-2200
Autosampler

L-2100/2130 e-Line cable

Pump

2-9
2.6 Assembly

(2) High-pressure Gradient System

e-Line cable
L-2450 DAD

Organizer
L-2300 Column
Oven e-Line cable

L-2200 L-2100/2130
Autosampler Pump

2.6.3 System Piping In the L-2000 series, provide inlet tubing to the pump and
waste solution tubing through the right piping section of the
drain reservoir, and tubing from the pump to the autosampler,
column and detector through the center piping section of the
drain reservoir.

Center piping section

Run tubes from the pump to the
autosampler, column and detector.
Drain reservoir

Right piping section

Run inlet tubing to the pump and
waste solution tubing.

2 - 10
 2.6.3

(1) Isocratic System

Washing solution Eluent

Inlet tube
Organizer

Detector cell

L-2450 DAD

L-2300 Column
Oven
Column

L-2200 Injection valve

Autosampler

Syringe valve

L-2100/2130
Pump

Drain
Degasser Drain valve

Connect the inlet tube for eluent to the IN port of the

degasser. Connect the OUT port of the degasser to the
inlet of the pump. Connect the drain valve of the pump
to port 1 of the injection valve of the autosampler.
Connect port 2 of the injection valve to the inlet of the
column. Connect the outlet of the column to the inlet
tube of the detector cell. For washing solution, provide
connection to port 1 of the syringe valve of the
autosampler through the degasser.

2 - 11
2.6 Assembly

(2) Low-pressure Gradient System

Washing solution Eluent

Inlet tube
Organizer

Detector cell

L-2450 DAD

L-2300 Column
Oven
Column
Injection valve

Syringe valve

L-2200
Autosampler

Mixer L-2100/2130
Pump

Degasser

Drain
Drain valve 4-way joint
Proportioning
valve

Connect the inlet tube for eluent to the IN port of the

degasser. Connect the OUT port of the degasser to the
IN port of the proportioning valve. Connect the OUT
port of the proportioning valve to the 4-way joint.
Connect the mixer of the pump to port 1 of the injection
valve of the autosampler. Connect port 2 of the injection
valve to the inlet of the column. Connect the outlet of
the column to the inlet tube of the detector cell.
For washing solution, provide connection to port 1 of the
syringe valve of the autosampler through the degasser.

2 - 12
 2.6.3

(3) High-pressure Gradient System

Inlet tube Detector cell

Eluent
Washing solution L-2450 DAD

L-2300 Column
Organizer Oven

Column Injection valve

Syringe valve
L-2100/2130
Pump L-2200
Autosampler

Drain
Drain valve

Connect the inlet tube for eluent to the IN port of the

degasser. Connect the OUT port of the degasser to the
IN port of the pump. Connect the drain valve of the
pump to the mixer. Connect of the mixer of the pump to
port 1 of the injection valve of the autosampler.
Connect port 2 of the injection valve to the inlet of the
column. Connect the outlet of the column to the inlet
tube of the detector cell. For washing solution, provide
connection to port 1 of the syringe valve of the
autosampler through the degasser.

2 - 13
2.6 Assembly

2.6.4 Instrument-to- The wiring cover is provided at the rear of each instrument.
instrument The instrument-to-instrument fastening can be made by
Fastening shifting each wiring cover as shown below.

(1) Loosen the retaining screws and detach the wiring cover.

Wiring cover

Retaining screw

(2) Shift the wiring cover and tighten the retaining screws.

Wiring cover

Retaining screw

2 - 14
 2.6.5

2.6.5 Removal of (1) Two transport screws are provided at the bottom of the
Transport Screw instrument. Remove them with a Phillips screwdriver.
and Sponge

Transport screw

The transport sponge is provided inside the instrument.

Detach the lamp cover at the front of the instrument.
Then, detach the Hg lamp lid and remove the transport
sponge (1 pc). For attachment of the Hg lamp, refer to
6.2.3.

Transport sponge

2 - 15
 3.1

3. FUNCTION

3.1 Front Panel

Power switch

LED

Fig. 3-1 Front Panel

Power switch : Turns ON/OFF power.

LED : Four LEDs are equipped, which provide
status indications given below.

Name (color) LED Status

CH1 (green/orange) Green : Use of CH1
Orange : Error in use of CH1
CH2 (green/orange) Green : Use of CH2
Orange : Error in use of CH2
D2 LAMP (green) Green : D2 lamp ON
W LAMP (green) Green : W lamp ON

3-1
3.2 Rear Panel

3.2 Rear Panel

e-Line
connector

Synchronous serial Power

communication connector
connector

Fig. 3-2 Rear Panel

No. Indication Function Reference

1 e-Line connector (1) Sends/receives instrument (1) Sending/receiving of
parameters through e-Line. instrument parameters
(2) Inputs/outputs contact
signals.
(a) START IN (2) (a) Start of program
(b) ERROR IN (b) Recognition of error in
other instrument
(c) ERROR OUT (c) Presentation of error
condition
(d) READY/BUSY OUT (d) Presentation of status
2 Synchronous Used to send acquired data to
serial USB interface.
communication
connector
3 Power Used to connect the power
connector cable.

3-2
 3.3

3.3 Operating Principle

W lamp

Lens

Converging
Flow cell mirror

Photodiode Lens
array
Switching
mirror
Slit

D2 lamp

Hg lamp

Grating

Fig. 3-3 Optical System Diagram

Figure 3-3 shows the optical system of the L-2450 DAD.

The light beam emitted from the D2 lamp or W lamp is
converged in the flow cell by the converging mirror.
After passing the flow cell, the beam enters the
monochromator through the slit. The beam is then dispersed
by the grating and converted into electrical signals by the
photodiode array (PDA).
For check of the wavelength accuracy, the switching mirror
shields the light beam from the D2 lamp or W lamp and reflects
that from the Hg lamp to introduce it into the detector.

3-3
3.4 Specifications

3.4 Specifications

(1) Optical System

Single beam photometry system (dispersion by diffraction

grating)

(2) Light Source

D2 lamp, W lamp, Hg lamp (for wavelength check)

(3) Detecting Element

1024-bit photodiode array

(4) Measuring Wavelength Range

190 to 900 nm (settable in steps of 1 nm)

(5) Wavelength Accuracy

1 nm

(6) Slit Type

2 types (FINE: 1 nm, COARSE: 4 nm)

(7) Noise

1.5 10-5 AU or less

(Conditions: Air in cell, spectral bandwidth 4 nm,
smoothed chromatogram 250 10 nm/
600 10 nm, sampling period 400 ms, time
constant 1.5 s, ambient temperature 25 C,
ambient temperature variation within 1 C,
after warming-up)

(8) Drift

0.5 10-3 AU/hr or less

(Conditions: Air in cell, spectral bandwidth 4 nm,
smoothed chromatogram 250 10 nm/
600 10 nm, ambient temperature 25 C,
ambient temperature variation within 1 C,
after warming-up)

3-4
 3.4

(9) Communication Function

e-Line (instrument parameters, instrument status)

Synchronous serial communication (acquired data)

(10) External Output Terminal (option)

Processor output (1 V in full scale)

Recorder output (10 mV in full scale)

(11) External Input/Output Contact Terminal

Time program start, error in/out, busy out (contact on e-

Line)

(12) Flow Cell

Quartz cell
(optical path length 10 mm, cell capacity 13 L)

(13) Withstand Pressure of Flow Cell

1.0 MPa

(14) Operating Temperature Range

4 to 35 C (condensation unallowable)

(15) Operating Humidity Range

45 to 85% (condensation unallowable)

(16) Power Supply

24 V DA

(17) Power Consumption

100 W

(18) Dimensions

340 (W) 400 (D) 150 (H) mm

(19) Weight

12 kg

3-5
 4.1

4. OPERATION

4.1 Turning On Power

Turn on the power switch of the instrument.

Power switch

Upon powering on the instrument, a self-diagnosis

(initialization) test is made.

NOTE: If an error message appears after turning on power,

then refer to 5.4 and 5.5.

4-1
4.2 Cautions on Operation

4.2 Cautions on Operation

For the L-2450 DAD, data acquisition parameters,

photometric values and error information are all
controlled/managed by the PC (EZChrom Elite for Hitachi).
For use of the instrument, it is necessary to make setting of
the PDA option in the Set Option window. (For details, refer
to the instruction manual for EZChrom Elite for Hitachi (control
option). A license is required separately for the three-
dimensional analysis option.)
In three-dimensional data acquisition, spectral data is
sequentially transferred from the L-2450 DAD to the PC.
During data acquisition, you can monitor a chromatogram in
real time on the PC. After completion of each analysis, three-
dimensional data is stored in the hard disk of the PC.
The three-dimensional chromatogram is used for peak purity
check, peak component identification or library data
preparation/search with the contour display and three-
dimensional chromatogram display functions on the PC screen.
In addition, an ordinary chromatogram is selected and
quantitative calculation is carried out for peaks in the
chromatogram. For the function and operation of EZChrom
Elite for Hitachi, refer to its instruction manual.

NOTE: If absorbance becomes high in absorptio metry,

the accuracy of photometric values will fall.
Linearity should be corrected by using a working
curve when analyzing samples of over 1 AU.
The linearity range may become narrower if an
eluent of large absorption is used.

NOTE: There are gaps between the cut filters at around

350 nm and 580 nm in this product. Since the
light volume is lower here than at other
wavelengths, steps may appear on a spectrum
in the following cases.
When a sample of large absorption near the
above wavelengths is measured.
When an eluent showing absorption near the
above wavelengths is used.
When a narrow bandpass (1 or 2 nm) is
selected.
When a fine slit is used.

4-2
 4.3

4.3 Shutdown Procedure

To shut down the instrument, turn off its power switch.

Note that the instrument should be shut down after termination
of EZChrom Elite for Hitachi.
The set measuring conditions are saved even when power is
turned on. Upon turning on power, you can make
measurement under the measuring conditions saved before
turning off power.

4.4 Washing after Use

After use of buffer solution, be sure to wash the flow cell with
distilled water. Otherwise the flow cell might be damaged
due to clogging with deposited salt.

4-3
 5.1.1

5. MAINTENANCE AND INSPECTION

5.1 Performance Check at Installation

5.1.1 Operation Check (1) Install the instrument according to Section 2.

(2) Turn on the power switch of the instrument and make

sure that the CH1 or CH2 LED is lit.

(3) Make sure that the D2 LAMP and W LAMP LEDs are lit.

(4) Start up EZChrom Elite for Hitachi and in the Instrument

Status window, make sure that the DAD is in Ready
status.

5.1.2 Performance Performance check at installation should be carried out under

Check the following conditions.
Install the instrument in a room where the temperature is 20
to 30 C and the temperature variation is within 1 C (at a
place where the instrument is not exposed to draft from an
air-conditioner or the like).
Warm up the instrument for at least one hour after turning
on power.
Fill the flow cell with air.

NOTE: If the inside of the flow cell is contaminated or not

dried completely, the instrument performance may
not be checked correctly. If the flow cell is
contaminated, wash it (refer to 5.3) before use.

For check of the noise level, use data which is small in

baseline drift.

(1) Check of Wavelength Accuracy

(a) Start up the maintenance software.

(b) Select System 1 or System 2.

(c) Click the Connect button and make sure that the
L-2450 Diode Array Detector icon appears.

5-1
5.1 Performance Check at Installation

(d) Click the Maintenance button.

(e) Click the WL Check button.

5-2
 5.1.2

(f) Select wavelengths to be checked and click the

Check Start button.

(g) Wavelength check is automatically carried out and

the wavelength deviation is displayed for each of the
selected wavelengths.

5-3
5.1 Performance Check at Installation

NOTE: If any wavelength is outside the calibration

range (indicated in blank) in wavelength
check results, set the calibration value to
the default one (83) in the calibration value
setting window and retry wavelength
accuracy check. (For details, refer to the
instruction manual for the maintenance
software.) If a wavelength outside the
calibration range is found again, contact
our service office.

(2) Check of Lamp Energy

(a) Click the Get button, and the respective energy

values of the D2 lamp and W lamp are displayed.

5-4
 5.1.2

(3) Check of Drift and Noise

For drift and noise check, use EZChrom Elite for Hitachi.
Upon completion of automatic configuration of Hitachi
LaChrom Elite, the automatically detected modules
appear in the Used Modules field. (For the detailed
operating procedure, refer to the instruction manual for
EZChrom Elite for Hitachi (control option).)
For use of the PDA option software, click the Options
button.

Select PDA in the Set Option window.

5-5
5.1 Performance Check at Installation

(a) Check the drift in the following procedure.

1) Open the Instrument Parameters dialog box and
click the DAD tab.

2) Set the following conditions in the Instrument

Parameters window.
Sampling time : 60 min
Sampling period : 400 ms
Wavelength range : 200 to 700 nm
Slit width : Coarse
Band width : 4 nm
3) Set the following data processing conditions in
the PDA Option window.
Multi-chromatogram:
Channel 1: Wavelength 250 nm, band width
20 nm
Channel 2: Wavelength 600 nm, band width
20 nm
4) Execute data acquisition and prepare
chromatograms at wavelengths of 250 nm and
600 nm.

5-6
 5.1.2

5) Calculate the drift at wavelengths of 250 nm and

600 nm.

Absorbance Time axis

Drift

60 minutes

(b) Check the noise level in the following procedure.

1) Set the following conditions in the Instrument
Parameters window.
Sampling time : 15 min
Sampling period : 400 ms
Wavelength range : 200 to 700 nm
Slit width : Coarse
Band width : 4 nm
2) Set the following data processing conditions in
the PDA Option window.
Multi-chromatogram:
Channel 1: Wavelength 250 nm, band width
20 nm
Channel 2: Wavelength 600 nm, band width
20 nm
3) Execute data acquisition and prepare
chromatograms at wavelengths of 250 nm and
600 nm.
4) Calculate the noise level at wavelengths of
250 nm and 600 nm.

5-7
5.2 Periodical Inspection

R =n
Noise level = YR n (Xn is in units of 0.5 min.)
R =1

Noise calculation (cited from ASTM E685-79.)

X1 X3 CENTER
X2

Y2 Y3
Y1
15 minutes

5.2 Periodical Inspection

No. Check Item Interval Check Procedure Remarks

1 Lighting of light Every day Check if D2 LAMP and W LAMP
source lamp LEDs come on.
2 Lifetime of light Every 1) Make energy check and ON See 5.1.2.
source lamp month time check.
2) Check if baseline drifts largely
(or baseline fluctuates or
noise becomes large).
3 Wavelength Every Check wavelength by utilizing Hg See 5.1.2.
accuracy year lamp and D2 lamp bright lines.

5-8
 5.3.1

5.3 Maintenance/Inspection Procedure

5.3.1 Check and

Washing of WARNING
Flow Cell
Ignition of Flammable Chemicals!

Beware of ignition hazard when using flammable

chemicals such as organic solvents.
Always check the flow path for the following points.
If an abnormality is found, stop operation immediately.
Leakage of solvent or waste solution
Leakage of solvent inside the instrument
Well-ventilate the laboratory room where the instrument
is used.
This instrument is not of an explosion-proof type.
Do not use organic solvents having an ignition point
below 70 C in unattended operation.
When using flammable chemicals, be careful about
possible ignition due to static electricity.
Particularly when using non-conductive chemicals,
employ a conductive vessel and provide a correct
grounding connection.

WARNING
Explosion of Vapor from Flammable Chemicals!

If a flammable chemical such as organic solvent leaks

from the flow path of the instrument and its vapor
concentration exceeds the explosion limit, it may cause
spontaneous combustion with dangerously explosive
results.
When using a flammable and readily volatile chemical,
be sure to check for leakage from the flow path of the
instrument and ventilate the laboratory room
adequately.

5-9
5.3 Maintenance/Inspection Procedure

(1) If the inside of the flow cell is contaminated, it should be

washed.

Items to be prepared for washing flow cell:

Injector
(made of glass and having a capacity of about 10 mL)
Washing solution

(2) Check and wash the flow cell in the following procedure.

(a) Loosen the two setscrews and remove the flow cell
cover.

Setscrew

Flow cell cover

(b) Loosen the two setscrews and remove the flow cell
lid.
Setscrews Flow cell lid

5 - 10
 5.3.1

Flow cell unit Flow cell spacer

(c) Pull out the flow cell spacer toward the front of the
detector.
Flow cell spacer

5 - 11
5.3 Maintenance/Inspection Procedure

(d) Pinch the center of the flow cell unit with your fingers
and with the flow cell pressed against the right (light
source side) of the instrument, take out the flow cell
by pulling it toward the front of the instrument.

Pressing direction

Pulling direction

(e) Visually check the inside of the flow cell for adhesion
of foreign matter (crystallized matter or the like) or
contamination.

(f) Visually check the flow cell for liquid leakage.

(g) If the inside of the flow cell is contaminated, it must

be washed. The washing solution to be used differs
depending on the eluent used. When the eluent is
an aqueous liquid, insert the injector filled with
distilled water into the tip of the drain tube and inject
the water into the cell for washing. When the eluent
is an organic solvent, fill the injector with ethanol
(acetone) and take the same procedure as described
above.

5 - 12
 5.3.1

Connecting tube
Drain tube (1.6 to 2 mm dia.)

Injector

(h) Replace the distilled water or ethanol (acetone) with

the eluent used and take the same procedure as in
step (f).

(i) If air bubbles are included in the flow cell, pump

liquid into it at a flow rate of 1.0 mL/min and a
pressure of 1.0 MPa or less, with the outlet of the
drain tube stopped up with your finger. Under this
status, pressurize the cell for 2 or 3 seconds and
then release the finger from the drain tube.
Air bubbles can be eliminated by repeating this
procedure 3 or 4 times.

From pump

NOTE: If the flow cell is pressurized excessively, it

may be damaged. Before cell washing,
be sure to set the pressure limiter of the
pump to 1.0 MPa max.

5 - 13
5.3 Maintenance/Inspection Procedure

(j) Insert the flow cell straight from the front of the
instrument and set it at a position where the
protrusion of the cell holder is retained in the hole
inside the instrument (with a click).

Cell holder

Protrusion

NOTE: Set the flow cell at the correct position.

Otherwise the instrument performance may be
degraded.

(k) Put the flow cell spacer into the instrument.

(l) Attach the flow cell lid and flow cell cover.

5 - 14
 5.3.1

5.3.2 Disassembly and

Washing of Flow WARNING
Cell
Ignition of Flammable Chemicals!

Beware of ignition hazard when using flammable

chemicals such as organic solvents.
Always check the flow path for the following points.
If an abnormality is found, stop operation immediately.
Leakage of solvent or waste solution
Leakage of solvent inside the instrument
Well-ventilate the laboratory room where the instrument
is used.
This instrument is not of an explosion-proof type.
Do not use organic solvents having an ignition point
below 70 C in unattended operation.
When using flammable chemicals, be careful about
possible ignition due to static electricity.
Particularly when using non-conductive chemicals,
employ a conductive vessel and provide a correct
grounding connection.

WARNING
Explosion of Vapor from Flammable Chemicals!

If a flammable chemical such as organic solvent leaks

from the flow path of the instrument and its vapor
concentration exceeds the explosion limit, it may cause
spontaneous combustion with dangerously explosive
results.
When using a flammable and readily volatile chemical,
be sure to check for leakage from the flow path of the
instrument and ventilate the laboratory room
adequately.

5 - 15
5.3 Maintenance/Inspection Procedure

If the flow cell is still contaminated after washing, disassemble

and wash it in the following procedure.

Retaining screw

Setscrew 1
080-3290

Seat
810-2697

Seat
810-2697

Setscrew 2
080-3290

(1) Loosen setscrews 1 and 2.

(2) Loosen the retaining screws.

5 - 16
 5.3.1

O-ring
L456005

Flow cell 10 mm
890-2394

Packing
060-3076

Cell holder
890-2221

(3) Take out the flow cell.

(4) Wash the flow cell with an ultrasonic cleaner.

(5) Wipe off contamination from the surface of the flow cell
using gauze moistened with acetone or ethanol.

(6) Align the upper and lower flow path holes in the flow cell
with the upper and lower screw holes in the cell holder.
Then insert the flow cell into the cell holder.

Mark
B A Upper side

Direction of
beam
Lower side
Direction of liquid You can see the flow path hole like
flow this from A side.

5 - 17
5.3 Maintenance/Inspection Procedure

(7) Reassemble the flow cell by reversing the disassembly

procedure.

(8) Flow liquid to check for leakage.

NOTE: Tighten the setscrews fully by hand and then

retighten them by about 30 with a double-end
wrench. Before check for liquid leakage, be
sure to set the pressure limiter of the pump to
1 MPa.

5 - 18
 5.4

5.4 Troubleshooting

For operation and maintenance/inspection of the personal

computer (PC), printer and HPLC unit, refer to their instruction
manuals.
Described here is the troubleshooting of the L-2450 DAD.

Judgement/
Symptom Cause Check Procedure Remedy

1. CH1 (or CH2) LED 1) Poor contact of Visual check Plug in power cord
does not come on power cord plug again.
when power switch is (system power unit
turned on. or AC adapter)
2) Improper attachment Visual check Retighten retaining
of lamp cover screw of lamp cover.
(activation of
protective circuit)
2. D2 LAMP (or W 1) Improper connection Visual check Reconnect lead wire of
LAMP) LED does not of lead wire of light light source lamp
come on. source lamp according to indication
on terminal block.
2) Blowout of light Check light source Replace light source
source lamp lamp for conductivity. lamp.
3. CH1 (or CH2) is lit in SYSTEM error Turn off power and Refer to error message
orange. then turn it on again. list.
If same symptom
appears, start up
maintenance software
of EZChrom Elite for
Hitachi and check error
message.
4. Noise is too large. 1) Contamination of Visual check Wash flow cell (see
flow cell 5.3.3).
2) Insufficient Appearance of spike- Deaerate solvent
deaeration of solvent like noise sufficiently.
3) Impurities contained Check solvent. Purify or replace
in solvent solvent.
4) Trouble on pump Check for regularity. Check pump.
Check for noise when
pump is stopped.
5) Expiration of lamp Make energy check of Replace lamp with a
life (D2/W) lamp (see 5.3.1). new one (see 6.2.1).
5. Drift is too large. 1) Insufficient Wait until instrument
warming-up becomes stable (for 1
hour or more).
2) Liquid leakage from Check joint for poor Retighten each joint.
joint tightening.
3) Elution of impurities Stop pumping liquid Wait until elution ends
from column and see what happens. completely or replace
column.
4) Variation in ambient Minimize variation in
temperature ambient temperature.
5) Low purity of solvent Check solvent for Purify or replace
purity. solvent.

5 - 19
5.5 Error Messages

5.5 Error Messages

On occurrence of an abnormality in operation of the L-2450

diode array detector, an error message is reported on the PC
display monitor.

Message Description Remedy

ROM ERROR message has been ROM error Contact the service
received from the L-2450 DAD. office.
Close the instrument icon and restart
the unit.
RAM ERROR message has been RAM error Contact the service
received from the L-2450 DAD. office.
Close the instrument icon and restart
the unit.
PARAMETER ERROR message has Parameter error (1) Turn on power again.
been received from the L-2450 DAD. (2) Contact the service
Close the instrument icon and try check office.
with the maintenance software and
condition change.
LOG INFORMATION ERROR message Log information error (1) Turn on power again.
has been received from the L-2450 (2) Contact the service
DAD. Close the instrument icon and office.
try check with the maintenance software
and condition change.
EEPROM ERROR message has been Instrument information (1) Turn on power again.
received from the L-2450 DAD. error (2) Contact the service
Close the instrument icon and try check office.
with the maintenance software and
condition change.
LAMP OFF BY EXTERNAL ERROR The lamp goes out due Turn on the lamp again.
message has been received from the to external error.
L-2450 DAD. Check the error
condition in the Status screen and try
remedial processing.
LAMP OFF BY LEAKAGE message has Occurrence of liquid Eliminate the cause of
been received from the L-2450 DAD. leakage. liquid leakage, wipe off
Check the error condition in the Status leaked liquid and turn on
screen and try remedial processing. the lamp.
DSP ERROR message has been Failure in DSP operation (1) Turn on power again.
received from the L-2450 DAD. (2) Contact the service
Close the instrument icon and restart office.
the unit.
D2 LAMP ERROR message has been 1. The D2 lamp blows 1.
received from the L-2450 DAD. out or fails to come (1) Turn on power again.
Check the error condition in the Status on. (2) Replace the D2 lamp
screen and try remedial processing. with a new one.
(3) Contact the service
office.
2. With the D2 lamp 2. If the cooling fan
alone turned on, the does not operate
cooling fan stops. after turning on
power again, contact
the service office.

5 - 20
 5.5

(contd)
Message Description Remedy
W LAMP ERROR message has been 1. The W lamp blows 1.
received from the L-2450 DAD. out or fails to come (1) Turn on power again.
Check the error condition in the Status on. (2) Replace the W lamp
screen and try remedial processing. with a new one.
(3) Contact the service
office.
2. With both the D2 2., 3.
lamp and W lamp If the cooling fan does
turned on, the not operate after turning
cooling fan stops. on power again, contact
3. With the W lamp the service office.
alone turned on, the
cooling fan stops.
Hg LAMP ERROR message has been The Hg lamp goes out or (1) Turn on power again.
received from the L-2450 DAD. fails to come on. (2) Replace the Hg lamp
Close the instrument icon and try check with a new one.
with the maintenance software and
condition change.
SLIT ERROR message has been Failure in slit switching (1) Turn on power again.
received from the L-2450 DAD. (2) Contact the service
Close the instrument icon and restart office.
the unit.
SHUTTER ERROR message has been Failure in shutter (1) Turn on power again.
received from the L-2450 DAD. operation (2) Contact the service
Close the instrument icon and restart office.
the unit.
DAC ERROR message has been Disruption of data (1) Turn on power again.
received from the L-2450 DAD. transfer to DAC (2) Contact the service
Close the instrument icon and restart office.
the unit.

5 - 21
 6.1

6. REPLACEMENT PARTS

6.1 Replacement Parts and Consumables

Table 6-1 lists the replacement parts and consumables used

in this instrument. Their lifetime changes remarkably
according to the operating condition of the instrument.
The lifetime is estimated on the assumption that the
instrument operates 160 hours/month (8 hour/day),
1920 hours/year (250 days).

Table 6-1 Replacement Parts and Consumables

Part No. Part Name Estimated Lifetime Remarks

885-3570 D2 lamp 1000 hr/6 months
890-2527 W lamp 1000 hr/6 months
890-2237 Hg lamp unit 100 hr/2 years
890-2394 Flow cell, 10 mm 9600 hr/ 5 years Cell alone
890-2289 Inlet tube, 9600 hr/5 years Length...................... 1 m
ID 0.25 0.66 T Outside diameter...... 1.57 mm
890-2288 Drain tube, 9600 hr/5 years Length...................... 2 m
ID 0.33 0.62 T Outside diameter...... 1.57 mm

6-1
6.2 Replacement of Lamp

6.2 Replacement of Lamp

WARNING
Electric Shock due to Contact with
Inside of Instrument!

Before removing the instrument cover for replacement of

parts or any other work, be sure to turn off the power
switch and unplug the power cord.

WARNING
Never Release Safety Mechanism!

In this instrument, power is automatically cut off with the

safety mechanism upon removing the lamp cover.
Avoid releasing the safety mechanism intentionally to
prevent an electric shock hazard which may be caused by
operational error.

CAUTION
Burns due to Contact with Hot Part!

The lamp and lamp cover are hot during use or

immediately after use. Touching the hot lamp cover or
lamp could result in burns.
Before lamp replacement, turn off the power switch and
wait for 30 minutes until the lamp cools down
sufficiently.

6-2
 6.2.1

6.2.1 Replacement of (1) Items to be Prepared

D2 Lamp New D2 lamp
Phillips screwdriver
Bladed-edged screwdriver

(2) Check Items

Make sure that the instrument is powered off.
Make sure that the power cord is unplugged from the
instrument.

(3) How to Replace D2 Lamp

(a) Detach the flow cell cover (2 retaining screws).

(b) Detach the lamp cover (2 retaining screws).

Flow cell cover Retaining screw

Retaining screw Lamp cover

6-3
6.2 Replacement of Lamp

(c) Loosen the three screws of the D2 lamp lead wires

connected to the terminal block and pull out the lead
wires.

Terminal
block

Lead wire

(d) Remove the three lamp cover retaining screws and

detach the lamp cover. (Remove each lead wire by
passing it through the hole in the cover.)

Lamp cover
retaining screw

Lamp cover

Lead wire

6-4
 6.2.1

(e) Loosen the three screws retaining the D2 lamp with a

blade-edged screwdriver (by two turns each).

Screw

(f) Detach the D2 lamp by turning it clockwise.

6-5
6.2 Replacement of Lamp

(g) Fit a new D2 lamp and turn it clockwise until there is

no more gap between the mounting hole and
retaining screw. Then tighten the three retaining
screws alternately (evenly) with the blade-edged
screwdriver.

D2 lamp

Retaining screw

Mounting hole There should

be no gap.
<Good Example> <Bad Example>

(h) Pass each lead wire through the hole in the cover.
Attach the cover.

(i) Connect the lead wires to the terminal block.

(j) Put back the lamp cover and flow cell cover in place.

(4) Operation Check

Plug the power cord into the instrument, turn on the

power switch and then make sure that the lamp is lit.

NOTE: If the lamp cover is attached improperly, the

lamp fails to come on.

(5) Check of Lamp Energy

(a) Warm up the instrument for 1 or 2 hours.

(b) Start up EZChrom Elite for Hitachi.

6-6
 6.2.1

(c) Click the Lamp Energy button in the Instrument

Status screen.

(d) Make sure that the displayed lamp energy value

(250 nm) is 15,000 counts or more.

6-7
6.2 Replacement of Lamp

(6) Resetting of Lamp Information

(a) Click the View Maintenance button in the Instrument

Status screen.

(b) Click the Reset button for the D2 lamp.

(c) The lamp ON time and ON count are reset to 0

(zero) and the replacement date to the current date
automatically.

6-8
 6.2.2

6.2.2 Replacement of (1) Items to be Prepared

W Lamp New W lamp
Phillips screwdriver
Bladed-edged screwdriver

(2) Check Items

Make sure that the instrument is powered off.
Make sure that the power cord is unplugged from the
instrument.

(3) How to Replace W Lamp

(a) Detach the flow cell cover (2 retaining screws).

(b) Detach the lamp cover (2 retaining screws).

Flow cell cover Retaining screw

Retaining screw Lamp cover

6-9
6.2 Replacement of Lamp

(c) Loosen the two lamp socket retaining screws of the

W lamp house and pull out the lamp socket toward
you.

Retaining screw

Lamp
socket

(d) Detach the W lamp from the lamp socket and attach
a new W lamp.

W lamp

NOTE: Avoid directly touching the glass surface of

the W lamp. For attachment of the lamp,
use soft gauze or the like.

(e) Attach the lamp socket to the W lamp house and

tighten the retaining screws.

(f) Put back the lamp cover and flow cell cover in place.

6 - 10
 6.2.2

(4) Operation Check

Plug the power cord into the instrument, turn on the

power switch and then make sure that the W lamp is lit.

NOTE: If the lamp cover is attached improperly, the

lamp fails to come on.

(5) Check of Lamp Energy

(a) Warm up the instrument for 1or 2 hours.

(b) Start up EZChrom Elite for Hitachi.

(c) Click the Lamp Energy button in the Instrument

Status screen.

(d) Make sure that the displayed lamp energy value

(600 nm) is 15,000 counts or more.

6 - 11
6.2 Replacement of Lamp

(6) Resetting of Lamp Information

(a) Click the View Maintenance button in the Instrument

Status screen.

(b) Click the Reset button for the W lamp. The lamp
ON time and ON count are reset to 0 (zero) and the
replacement date to the current date automatically.

6 - 12
 6.2.3

6.2.3 Replacement of (1) Items to be Prepared

Hg Lamp New Hg lamp unit
Phillips screwdriver

(2) Check Items

Make sure that the instrument is powered off.
Make sure that the power cord is unplugged from the
instrument.

(3) How to Replace Hg Lamp

(a) Detach the flow cell cover (2 retaining screws).

(b) Detach the lamp cover (2 retaining screws).

Flow cell cover Retaining screw

Retaining screw Lamp cover

6 - 13
6.2 Replacement of Lamp

(c) Detach the Hg lamp lid (2 retaining screws).

Hg lamp lid

Retaining screw

(d) Disconnect the lead wire connector of the Hg lamp

unit from the lamp inverter.

Connector Setscrew

6 - 14
 6.2.3

(e) Loosen the setscrew of the Hg lamp unit and detach

it from the monochromator base.

Hg lamp unit

NOTE: The lamp is a high-voltage part. So take

care when handling it.

Hg lamp unit

(f) Attach a new Hg lamp unit to the monochromator

base and tighten the setscrew.

(g) Put back the Hg lamp lid in place. After that,

reattach the lamp cover and flow cell cover.

6 - 15
6.2 Replacement of Lamp

(4) Operation Check

(a) Start up the maintenance software of EZChrom Elite

for Hitachi.

(b) Execute DAD wavelength check and in the spectrum

display window, make sure that a sharp peak
appears in the vicinity of 253.7 nm.

6 - 16
 6.2.3

(5) Resetting of Lamp Information

(a) Click the View Maintenance button in the Instrument

Status screen.

(b) Click the Reset button for the Hg lamp. The lamp
ON time and ON count are reset to 0 (zero) and the
replacement date to the current date automatically.

6 - 17
 APPENDIX 1. DESCRIPTION OF CONTACT
SIGNAL COMMUNICATION

1. Outline

The following two types of contact signals are used in the

L-2000 series.

(1) Contact Signals Entering e-Line Connector

(START, ERROR, BUSY)

These are contact signals having input/output functions

that are used in common for each unit. These signals
are respectively formed from the START (IN/OUT),
ERROR (IN/OUT) and BUSY (IN/OUT).

(2) Individual Contact Signals Used by Each Unit

These are contact signals that are input and output via
the 3-pin connector. Their names and functions vary
with the unit. A name that indicates the function is given
at each connector.

Example of output contact signal : EVENT of pump

Example of input contact signal : LAMP OFF IN of
detector

APPENDIX - 1
2. Contact Signals Entering e-Line Connector

Each unit has two e-Line connectors for connection at the rear
of the main unit, and their signals are internally connected.
By connecting each unit in series via the e-Line cable,
therefore, synchronism of the L-2000 series system can be
easily achieved.
For obtaining synchronism with the L-7000 series and/or
D-2500 by way of contact signals, use the cables listed below
for connection.

(1) Connection with L-7000 Series

Use 3-pin connectors of the e-DIO cable (890-6145) and

SDIO cable (810-7631) in the connection shown below.

START
ERROR STOP

To L-2000 To L-7000
series series
instrument instrument
e-Line D-line
connector connector
BUSY 3-pin BUSY
connector

e-DIO cable SDIO cable

(890-6145) (890-7631)

APPENDIX - 2
 (2) Connection with Model D-2500

Use the e-DIO cable (890-6146) for connection.

D-2500
H
L

START START
EXTERNAL
To L-2000 series COM
instrument G H
BUSY
BUSY L

e-Line connector

Connect the START L and G terminals of the e-DIO cable

(890-6146) to the EXTERNAL-COM terminal of the
D-2500.

APPENDIX - 3
3. Individual Contacts of Each Unit

(1) Contact Signal Input Terminals

These are contact signals input terminals for control

using contact signals from an external switch, relay, etc.
Any of the signals is activated by shorting the terminal for
0.1 second or longer. The input circuit is shown in
Fig. 1.

(2) Contact Signal Output Terminals

These are contact signal output terminals for control

using contact signals from an external unit. The output
circuit is shown in Fig. 4. The rating of contacts used is
12 V, 0.1 A. Make sure that the load connected does
not exceed this rating.

e-Line cable Other unit

L-2000 series unit A L-2000 series unit B

Fig. 1 Connection of e-Line Cable

APPENDIX - 4
 +5 V

320
IN

10 mA max.
per unit
OUT 4.7 k
(H) 12 V max.
ON : 5V 0.1 A max. (reverse polarity
OFF : 0V prohibited)
(L)

(G)

Fig. 2 Circuit Configuration of e-Line Contact

+5 V

320
1

10 mA max.
2

L-2000 series Activated upon shorting External unit

contacts of external unit.

Fig. 3 Contact Signal Input Circuit

APPENDIX - 5
 0.1 A max.
1

30 V max.
2

L-2000 series External unit

Fig. 4 Contact Signal Output Circuit

APPENDIX - 6
APPENDIX 2. TERMINOLOGY

EZChrom Elite for Hitachi

Software for control of the whole HPLC system, data

acquisition, data analysis and data management.

Slit

An optical element used to let in the required part of the

light.

Instrument parameters

Parameters for determining the operating conditions of the

instrument, which can be printed in list format when the
D-7500 Data Processor is connected.

Diode array detector (DAD)

A detector for HPLC that employs a photodiode array as a

detector.

Download

To send parameters, etc. from the upper control unit

(EZChrom Elite for Hitachi for example).

Default

Initial setting when no specific setting is input.

D2 lamp

A deuterium lamp, which is used as a light source lamp.

Filter

An optical element used to let in only the light at the

required wavelength.

APPENDIX - 7
 Photodiode array (PDA)

A detector in which semiconductor photosensor elements

are lined up.

Grating

An optical element that disperses the light according to the

wavelength.

Flow cell

A measuring vessel for continuously measuring the sample.

Lamp house

A lamp housing, which becomes hot when the instrument is

in use. Take care not to be burned.

Wavelength calibration

To check the wavelength accuracy.

Hg lamp

A mercury lamp, which is used for wavelength calibration.

Validation

To check if the performance of the instrument remains

normal.

APPENDIX - 8