Raw material exclusively for cosmetic manufacturing

List of major products

Product name Concentration Source INCI Name Other components Package

Concentration(1%)
CITRIC ACID, SODIUM CITRATE,
Atelocollagen 1% 2% Bovine WATER, METHYLPARABEN OR 1Kg
SOLUBLE PHENOXYETHANOL
COLLAGEN Concentration(2%)
CITRIC ACID, WATER,
Porcine Atelocollagen 1% 2% Porcine METHYLPARABEN OR 1Kg

ATELOCOLLAGEN
PHENOXYETHANOL

AtelocollagenSS 0.3% 1% 2% Bovine DISODIUM PHOSPHATE, 1Kg

POTASSIUM PHOSPHATE,
SUCCINOYL WATER
Porcine AtelocollagenSS 0.3% 1% 2% Porcine 1Kg
ATELOCOLLAGEN METHYLPARABEN OR
PHENOXYETHANOL
Shark AtelocollagenSS 0.3% 1% Shark 1Kg

CELLULOSE GUM,
Porcine AtelocollagenSS-V 0.3% Porcine 1Kg
SUCCINOYL DISODIUM PHOSPHATE,
ATELOCOLLAGEN POTASSIUM PHOSPHATE
Shark AtelocollagenSS-V 0.3% Shark PHENOXYETHAOL, WATER 1Kg

AtelocollagenMS 1% Bovine MYRISTOYL DISODIUM PHOSPHATE, 1Kg

SUCCINOYL POTASSIUM PHOSPHATE,
ATELOCOLLAGEN WATER, METHYLPARABEN 1Kg
Porcine AtelocollagenMS 1% Porcine

DISODIUM PHOSPHATE,
SOLUBLE
Porcine AtelocollagenH 1.5% Porcine POTASSIUM PHOSPHATE 1Kg
COLLAGEN
WATER, METHYLPARABEN

List of sources of products

Material*
Product name Bovine Porcine Shark

Atelocollagen --
AtelocollagenSS
AtelocollagenSS-V --
AtelocollagenMS --
AtelocollagenH -- --
* None of the products is a mixture of more than one ingredient. : Available -- : Not available

Characteristics Applications
Highly safe bovine dermis from Australia, which is used for medical collagen, is used as the raw material for products of bovine origin.
The skin of healthy pigs that passed the test according to "Article 10 of the Japanese Slaughterhouse Act" is used as the raw material for products of porcine origin.
The dermis of fresh Prionace glauca landed at Kesennuma, Miyagi Prefecture is used as the raw material for products of shark origin. Atelocollagen is a hydrophilic Lotions, Milky lotions,
The content in this document is prepared based on documents and experimental data available at the time; however, the content does not comprise a quarantee. Please protein with a high moisture Creams, Skin care products,
note in advance that the content described in this document may be changed depending on the circumstances. All intellectual property rights including copyrights and
design rights of data described here and related documents belong to Koken Co., Ltd., and any unauthorized reproduction, reprinting or citation is strictly prohibited. Please
refer to the MSDS of the product in advance for the safe use of these ingredients. In addition, care should be taken to comply with the laws and regulations related to the retention capacity. etc.
Pharmaceutical Aairs Law in each country on expressions concerning consumer products.

1-4-14 Koraku, Bunkyo-ku, Tokyo 112-0004, Japan Tel: 81-3-3816-3562 Fax: 81-3-3816-3582 E-mail:customer@kokenmpc.co.jp C-1-805-7-01-00
Name and molecular diagrams of collagen contained in products Denaturing temperature of atelocollagen
Collagen Figure 2. Dierences in the denaturing temperature between animal species
(Native) Gelatin
NH 2 CONH 2

Alkali-treated collagen Atelocollagen has a triple helix structure, which is

COOH NH 2 Bovine
Thermal denaturation, etc. loosened by predetermined heating, turning it into
NH 2 COOH
Alkali treatment N-Telopeptide Triple-helix region C-Telopeptide Porcine
gelatin. This is called denaturation, and the tempera-
Shark
ture at which this occurs is called the denaturation

Specic rotation
COOH Enzyme
NH 2 treatment Pink Salmon temperature.
Atelocollagen H Hydrolyzed collagen
(collagen peptide) The denaturation of collagen can be observed by
Atelocollagen
Hydrolysis/enzyme treatment specific rotation (Figure 2). The denaturation
Atelocollagen from which telopeptides have NH 2 CONH 2
temperature is different for different animal species,
been removed is a collagen that is less
and tends to be higher in organisms that live in a
antigenic and more biocompatible than COOH NH 2
warm climate, and lower in organisms that live in a
collagen (native), gelatin or collagen peptide. Atelocollagen Modication cold climate (Figure 2).
(myristoylation/succinylation)
Temperature
Modication (succinylation)
Succinyl atelocollagen
CO CH 2 CH 2 COOH
Succinyl atelocollagen Myristoyl/succinyl atelocollagen
Relationship between atelocollagen solubility and pH
NH CONH 2 Addition of thickener
CO CH 2 CH 2 COOH CO CH 2 CH 2 COOH
NH CONH 2 NH CONH 2
COOH NH 2

AtelocollagenSS-V Figure 3. Dierence in the relationship Table 2. Solubility of atelocollagen

COOH NH 2 COOH NH CO (CH 2)12 CH 3 between solubility and pH
Isoelectric point Acidic Neutral Basic
Product name (pI) solution solution solution
Atelocollagen SS Atelocollagen MS
Product name

Atelocollagen 9.1
AtelocollagenSS 4.5
AtelocollagenSS-V 4.5

Comparison of atelocollagen among animal species AtelocollagenMS 4.5

Turbidity
AtelocollagenH 5.1

Amino acids that form the triple helix The solubility of Atelocollagen, SS, SS-V, MS, and H differs based on the
Table 1. Amino acid composition Figure 1. Molecular weight Atelocollagen
structure (glycine, alanine and proline) pH of the solvent. Atelocollagen is soluble in an acidic solution, and is
distribution by SDS-PAGE Atelocollagen SS
Porcine Bovine Shark account for about one third of the likely to be deposited in a neutral to basic solution. On the other hand,
Aspartic acid amino acid composition of atelocolla- Atelocollagen SS, SS-V, MS and H are soluble in neutral to basic
Threonine gen of porcine, bovine and shark origin. solutions.
Serine MW 300,000 The amino acid composition of atelocol-
(gamma chain)
Glutamic acid
lagen of shark origin consists of slightly
less hydroxyproline and proline and
Glycine
slightly more isoleucine and methionine

Emulsication by Atelocollagen MS
Alanine
than those of porcine and bovine origin.
Cystine
However, there is not much difference in
Valine
MW 200,000 the amino acid composition between
Methionine (beta chain)
animal species (Table 1). Figure 4. Comparison of emulsication by atelocollagen
Isoleucine
While the molecular weight of atelocol- Figure 4 shows 2 g each of collagen, PEG600, and liquid paraffin
Leucine A: Atelocollagen MS B: Atelocollagen
lagen is slightly different due to the mixed with a homogenizer and left to rest at room temperature for 24
Tyrosine
Phenylalanine
differences in the amino acid composi- A B hrs. Emulsification was maintained after leaving to rest for 24 hrs
tion, there are hardly any differences in when atelocollagen MS was added (A). On the other hand, the liquid
Hydroxylysine MW 100,000 the molecular weight distribution
(alpha chain) separated and emulsification could not be maintained when ateloco-
Lysine patterns between animal species
llagen was added (B).
Histidine (Figure 1).
Atelocollagen MS is atelocollagen modified with lipid, and is a
Arginine
cosmetic ingredient that has both hydrophilicity inherent to collagen
Hydroxyproline
Our atelocollagen is a highly pure product and lipophilicity due to lipid modification (amphiphilicity). It can be
Proline
that has been purified to remove used as an ingredient with a moisturizing effect and an emulsifying
Total
degradation products and impurities effect in milky lotions, etc.
(Figure 1).