Differentiation of Enterobacter with a KIA/TSIA reaction of Acid/Acid,

With/Without Gas

Serratia marcescens
Testing Media Reaction
H2S (KIA/TSIA). -
Carbohydrate Fermentations
Glucose and Gas A
Lactose.. v-4
Sucrose.. A
Manitol.. A
Dulcitol. -
Salicin. A
Adonitol V
Inositol.. V-
Sorbitol. A
Arabinose.... -
Raffinose.. -
Rhammose.. -
Melibiose.. -
Trehalose. A
Xylose. -
Amygdalin NR
IMViC Indole..
Methyl Red.. V
Voges Proskaurer +
Simmons Citrate +
Malanote -
Gloconate.. +
Chistensens Urease VW
Motility. +
Gelation Liquofaction 22oC V+
KCN broth-growth...... +
Arginin dihydrolase NH3 -
Lysine Decarboxylase.. +
Ornithine Decarboxylase +
Phenylalanine Deaminase.. -
ONPG.. +
Esculin +
Coloni pigmentation.. V

1
possible organisme KIA : Escherichia, Klebsiella or Enterobacter spp; TSIA : Eschericia, Klebsiella, Enterobacter, Serratia or
Yersiniaenterocolitica
2
Differentation of three species of Klebsiella 3 Negative H 2
S on KIA or TSIA but may be weakly peptone leon agar medium
4 5
may be delayed Yellow, nondiffused
6
Non water solute , pink red magenta pigmen temperature (220C), rerely at (37oC) slow
 Differentiation of Enterobacter with a KIA/TSIA reaction of Acid/Acid,
With/Without Serratia rubidaea
Gas

Testing Media Reaction

H2S (KIA/TSIA). -
Carbohydrate Fermentations
Glucose and
A
Gas
Lactose.
A
.
Sucrose
A
..
Manitol.
A
.
Dulcitol. -
Salicin
V-4
.
Adonitol V-
Inositol.
V-
.
Sorbitol. -
Arabinose
A
.
Raffinose.
A
.
Rhammose.
-
.
Melibiose.
A
.
Trehalose. A
Xylose
V
.
Amygdalin
NR

IMViC Indole.. -
Methyl
V
Red..
Voges Proskaurer +
Simmons
+
Citrate
Malanote
V-

1
possible organisme KIA : Gloconate Escherichia, Klebsiella or
NR
Enterobacter spp; TSIA : .. Eschericia, Klebsiella,
Enterobacter, Serratia or Chistensens Urease VW Yersiniaenterocolitica
2 3
Differentation of three species Motility. V+ of Klebsiella Negative
2
H S on KIA or TSIA but may be
weakly peptone leon agar
Gelation Liquofaction 22oC VW medium
4
may be delayed KCN broth-growth... V+
Arginin dihydrolase NH3 - 5
Yellow, nondiffused
6
Non water solute , pink red Lysine Decarboxylase.. V4 magenta pigmen temperature
(220C), rerely at (37oC) slow
Ornithine Decarboxylase -
Phenylalanine Deaminase.. -
ONPG
NR
..
Esculin
NR

Coloni pigmentation.. -
 Differentiation of Enterobacter with a KIA/TSIA reaction of Acid/Acid,
With/Without Gas

Yersinia

Testing Media Reaction

1 H2S (KIA/TSIA). -
possible organisme KIA : Escherichia, Klebsiella or
Enterobacter spp; TSIA : Carbohydrate Fermentations Eschericia, Klebsiella,
Enterobacter, Serratia or Glucose and Yersiniaenterocolitica
A
2
Differentation of three Gas species of Klebsiella
3
Negative H Lactose. 2
S on KIA
or TSIA but may be weakly - peptone leon agar medium
.
4
may be delayed Sucrose.
A 5
Yellow, nondiffused
6 .
Non water solute , pink red magenta pigmen temperature
(220C), rerely at (37oC) slow Manitol.. A
Dulcitol. -
Salicin
.
Adonitol
Inositol..
Sorbitol. A
Arabinose.... V
Raffinose.. -
Rhammose.. -
Melibiose.
-
.
Trehalose. A
Xylose
V
.
Amygdalin
NR

IMViC Indole.. V-
Methyl Red..
Voges Proskaurer
Simmons
Citrate
Malanote

Gloconate
NR
..
Chistensens Urease V
Motility.
Gelation Liquofaction 22oC
KCN broth-growth......
Arginin dihydrolase NH3
Lysine Decarboxylase..
Ornithine Decarboxylase
Phenylalanine Deaminase..
ONPG.
.
Esculin

Coloni pigmentation..
Differentation of Enterobacteriaceae with a KIA/TSIA reaction of
Alkaline/Acid, H2S, With/Without Gas
 Salmonella thypi
Testing Media Reaction
H2S (KIA/TSIA). +
Carbohydrate Fermentations
Glucose and
A
Gas..
Lactose
-
.
Sucrose
V-
.
Manitol.
-

Dulcitol
-

Salicin
-

Adonitol.
-
.
Inositol... -
Sorbitol
-

Arabinose...
V-

Raffinose
-
.
Rhammose
-
.
Melibiose
-

Trehalose
-

Xylose.
-
.
Amygdalin. -
IMViC Indole. +
Methyl
+
Red.
Voges Proskaurer.. -
Simmons Citrate. -
Malanote
-
.
Gloconate
-

Chistensens
-
Urease..
Motility
+

Gelation Liquofaction 22oC.. -

KCN broth-growth..... -
Arginin dihydrolase NH3.. -
Lysine Decarboxylase. +
Ornithine Decarboxylase.. +
Phenylalanine Deaminase.... -
ONPG
-

Esculin
-
.