Description

StrainDesignation:3147[CBS6431,CCY293106,CIP48.72,DSM1386,IFO1594,NCPF3179,NCYC
1363,NIH3147,VTTC85161]
ProductSheet DepositedName:Candidaalbicans(Robin)Berkhout
AntigenicProperties:SerotypeA
Candidaalbicans(ATCC ProductDescription:Anampoulecontainingviablecells(mayincludesporesandmycelia)suspendedin
cryoprotectant.
10231)
Propagation

PleasereadthisFIRST Theinformationrecommendedinthissectionistoassistusersinobtaininglivingculture(s)fortheirstudies.
Therecommendationdoesnotimplythattheconditionsorproceduresprovidedbelowareoptimum.
StorageTemp. Experiencedresearchersmayinitiatethegrowthofacultureintheirownway.
Frozen:80Cor
colder ATCCMedium200:YMagarorYMbroth
FreezeDried:2C ATCCMedium28:Emmons'modificationofSabouraud'sagar

to8C ATCCMedium1245:YEPD

LiveCulture:See
Propagation GrowthConditions
Section Temperature:24Cto26C
Atmosphere:Typicalaerobic

RecommendedProcedure

BiosafetyLevel Forfreezedry(lyophilized)ampoules:
1 1. Openanampouleaccordingtoenclosedinstructions.
2. Fromasingletesttubeofsteriledistilledwater(5to6mL),withdrawapproximately0.5to1.0mL
withasterilepipetteandapplydirectlytothepellet.Stirtoformasuspension.
3. Asepticallytransferthesuspensionbackintothetesttubeofsteriledistilledwater.
IntendedUse
4. Letthetesttubesitatroomtemperature(25C)undisturbedforatleast2hourslonger(e.g.,
overnight)rehydrationmightincreaseviabilityofsomefungi.
Thisproductisintendedforresearchuseonly.Itisnot
5. Mixthesuspensionwell.Useseveraldrops(ormakedilutionsifdesired)toinoculaterecommended
intendedforanyanimalorhumantherapeuticor
solidorliquidmedium.Includeacontrolthatreceivesnoinoculum.
diagnosticuse.
6. Incubatetheinoculumatthepropagationconditionsrecommended.
7. Inspectforgrowthoftheinoculum/strainregularly.Thesignofviabilityisnoticeabletypicallyafter12
CitationofStrain daysofincubation.However,thetimenecessaryforsignificantgrowthwillvaryfromstraintostrain.

Ifuseofthiscultureresultsinascientificpublication,it
ColonyandCellMorphology:OnYEPDagarafter2daysat25C,coloniesarecreamcolored,shiny,and
shouldbecitedinthatmanuscriptinthefollowing
smooth.Oldercoloniesshowfilamentslikestructureatthemarginandmayhaveridgesorfolders.Cellsare
manner:Candidaalbicans(ATCC10231)
ovoid(3.06.0x4.08.0m),budding,mostlysinglyandrarelyclusteredinyoungculture.Cellswillelongate
andformchainlikebranchedpseudohyphaeinolderculture.

Notes

ThisstrainisrecommendedbyATCCforuseinthetestsdescribedinASTMStandardTestMethodE97991
whereonlythetaxonisspecifiedForsterilitytesting,notmorethanfivepassagesfromtheATCCculture
shouldbeusedPurifiedgenomicDNAofthisstrainisavailableasATCC10231D5.
Additional,updatedinformationonthisproductmaybeavailableontheATCCwebsiteatwww.atcc.org.

DNASequence

18SribosomalRNAgene,partialsequenceinternaltranscribedspacer1,5.8SribosomalRNAgene,
andinternaltranscribedspacer2,completesequenceand26SribosomalRNAgene,partialsequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACTGATTTGCTTAATTGCACCACATGTGTTTTTCTTT
GAAACAAACTTGCTTTGGCGGTGGGCCCAGCCTGCCGCCAGAGGTCTAAACTTACAACCAATTTTTTAT
AmericanTypeCultureCollection
CAACTTGTCACACCAGATTATTACTAATAGTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGA
POBox1549
Manassas,VA20108USA
TGAAGAACGCAGCGAAATGCGATACGTAATATGAATTGCAGATATTCGTGAATCATCGAATCTTTGAA
www.atcc.org CGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTTGAGCGTCGTTTCTCCCTCAAACCGCTGG
GTTTGGTGTTGAGCAATACGACTTGGGTTTGCTTGAAAGACGGTAGTGGTAAGGCGGGATCGCTTTGA
800.638.6597or703.365.2700 CAATGGCTTAGGTCTAACCAAAAACATTGCTTGCGGCGGTAACGTCCACCACGTATATCTTCAAACTTT
Fax:703.365.2750
GACCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAA
Email:Tech@atcc.org

Orcontactyourlocaldistributor D1D2regionofthe26SribosomalRNAgene
ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAGCGGCGAGTGAAGCGGCAAA
Page1of2
 AGCTCAAATTTGAAATCTGGCGTCTTTGGCGTCCGAGTTGTAATTTGAAGAAGGTATCTTTGGGCCCGG
CTCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGACCCGG
GTCTGTGTAAAGTTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCA
TCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAAC
TTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCTTGAGATCAGACTTGGTAT
TTTGCATGCTGCTCTCTCGGGGGCGGCCGCTGCGGTTTACCGGGCCAGCATCGGTTTGGAGCGGCAGG
ProductSheet
ATAATGGCGGAGGAATGTGGCACGGCTTCTGCTGTGTGTTATAGCCTCTGACGATACTGCCAGCCTAG
Candidaalbicans(ATCC ACCGAGGACTGCGGTTTTTAACCTAGGATGTTGGCATAATGATCTTAA

10231) Isolation

Manwithbronchomycosis
PleasereadthisFIRST
References
StorageTemp.
Frozen:80Cor Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.
colder
FreezeDried:2C BiosafetyLevel:1

to8C
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
LiveCulture:See
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
Propagation
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
Section forHealth.

ATCCWarranty

BiosafetyLevel
ATCCproductsarewarrantedfor30daysfromthedateofshipment,andthiswarrantyisvalidonlyifthe
1
productisstoredandhandledaccordingtotheinformationincludedonthisproductinformationsheet.Ifthe
ATCCproductisalivingcellormicroorganism,ATCCliststhemediaformulationthathasbeenfoundtobe
effectiveforthisproduct.Whileother,unspecifiedmediamayalsoproducesatisfactoryresults,achangein
IntendedUse mediaortheabsenceofanadditivefromtheATCCrecommendedmediamayaffectrecovery,growthand/or
functionofthisproduct.Ifanalternativemediumformulationisused,theATCCwarrantyforviabilityisno
Thisproductisintendedforresearchuseonly.Itisnot longervalid.
intendedforanyanimalorhumantherapeuticor
diagnosticuse.
Disclaimers

CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Candidaalbicans(ATCC10231)
Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproductsheet,
ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureand
patentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeen
confirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.ATCC
isnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.Whilereasonableeffort
ismadetoinsureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfrom
themisidentificationormisrepresentationofsuchmaterials.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2017.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[01/12]

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org

800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page2of2