Description

StrainDesignation:S288C
DepositedName:SaccharomycescerevisiaeHansen
ProductSheet Genotype:MATalphaSUC2malmelgal2CUP1flo1flo81hap1
ProductDescription:Anampoulecontainingviablecellssuspendedincryoprotectant.
Saccharomycescerevisiae
(ATCC204508) Propagation

Theinformationrecommendedinthissectionistoassistusersinobtaininglivingculture(s)fortheirstudies.
Therecommendationdoesnotimplythattheconditionsorproceduresprovidedbelowareoptimum.
PleasereadthisFIRST Experiencedresearchersmayinitiatethegrowthofacultureintheirownway.

StorageTemp. ATCCMedium1069:YPADmedium
Frozen:80Cor
colder
GrowthConditions

FreezeDried:2C Temperature:25Cto30C
to8C Atmosphere:Typicalaerobic
LiveCulture:See
Propagation RecommendedProcedure
Section Frozenampoulespackedindryiceshouldeitherbethawedimmediatelyorstoredinliquidnitrogen.Ifliquid
nitrogenstoragefacilitiesarenotavailable,frozenampoulesmaybestoredatorbelow70Cfor
approximatelyoneweek.Donotunderanycircumstancestorefrozenampoulesatrefrigerator
freezertemperatures(generally20C).Storageoffrozenmaterialatthistemperaturewillresultinthe

BiosafetyLevel deathoftheculture.
1 1. Tothawafrozenampoule,placeina25Cto30Cwaterbath,untiljustthawed(approximately5
minutes).Immersetheampoulejustsufficienttocoverthefrozenmaterial.Donotagitatethe
ampoule.
2. Immediatelyafterthawing,wipedownampoulewith70%ethanolandasepticallytransferatleast50
IntendedUse L(or23agarcubes)ofthecontentontoaplateorbrothwithmediumrecommended.
3. Incubatetheinoculum/strainatthetemperatureandconditionsrecommended.
Thisproductisintendedforresearchuseonly.Itisnot 4. Inspectforgrowthoftheinoculum/strainregularly.Thesignofviabilityisnoticeabletypicallyafter13
intendedforanyanimalorhumantherapeuticor daysofincubation.However,thetimenecessaryforsignificantgrowthwillvaryfromstraintostrain.
diagnosticuse.


CitationofStrain
Notes
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
Nospecialnotes.
manner:Saccharomycescerevisiae
Additional,updatedinformationonthisproductmaybeavailableontheATCCwebsiteatwww.atcc.org.
(ATCC204508)

DNASequence

18SribosomalRNAgene,partialsequenceinternaltranscribedspacer1,5.8SribosomalRNAgene,
andinternaltranscribedspacer2,completesequenceand28SribosomalRNAgene,partialsequence
CATTAAAGAAATTTAATAATTTTGAAAATGGATTTTTTTGTTTTGGCAAGAGCATGAGAGCTTTTACTGG
GCAAGAAGACAAGAGATGGAGAGTCCAGCCGGGCCTGCGCTTAAGTGCGCGGTCTTGCTAGGCTTGT
AAGTTTCTTTCTTGCTATTCCAAACGGTGAGAGATTTCTGTGCTTTTGTTATAGGACAATTAAAACCGTTT
CAATACAACACACTGTGGAGTTTTCATATCTTTGCAACTTTTTCTTTGGGCATTCGAGCAATCGGGGCCC
AGAGGTAACAAACACAAACAATTTTATCTATTCATTAAATTTTTGTCAAAAACAAGAATTTTCGTAACT
GGAAATTTTAAAATATTAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAG
CGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGC
CCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCCTTCTCAAACATTCTGTTTGGTAGTGAGT
GATACTCTTTGGAGTTAACTTGAAATTGCTGGCCTTTTCATTGGATGTTTTTTTTCCAAAGAGAGGTTTCT
CTGCGTGCTTGAGGTATAATGCAAGTACGGTCGTTTTAGGTTTTACCAACTGCGGCTAATCTTTTTTTATA
AmericanTypeCultureCollection CTGAGCGTATTGGAACGTTATCGATAAGAAGAGAGCGTCTAGGCGAACAATGTTCTTAAAGTTTGACC
POBox1549
TCAAATCAGGTAGGAGTACCCGCTGAACTTAAG
Manassas,VA20108USA
www.atcc.org
D1D2regionofthe28SribosomalRNAgene
800.638.6597or703.365.2700 AATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTC
Fax:703.365.2750 AAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTT
Email:Tech@atcc.org
GTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTT

Orcontactyourlocaldistributor GTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAA
AGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGA
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 AAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGTGTTTTGT
GCCCTCTGCTCCTTGTGGGTAGGGGAATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGAT
AAATCCATAGGAATGTAGCTTGCCTCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTG
AGGACTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCG

ProductSheet Isolation

Saccharomycescerevisiae Wildtypestrains:parentalstrainsinwhichmanyofthemutationshavebeenderived(AA2)

(ATCC204508)
References

Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.
PleasereadthisFIRST
BiosafetyLevel:1
StorageTemp.
Frozen:80Cor Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
colder thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
FreezeDried:2C DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes

to8C forHealth.

LiveCulture:See
ATCCWarranty
Propagation
Section
ATCCproductsarewarrantedfor30daysfromthedateofshipment,andthiswarrantyisvalidonlyifthe
productisstoredandhandledaccordingtotheinformationincludedonthisproductinformationsheet.Ifthe
ATCCproductisalivingcellormicroorganism,ATCCliststhemediaformulationthathasbeenfoundtobe
BiosafetyLevel effectiveforthisproduct.Whileother,unspecifiedmediamayalsoproducesatisfactoryresults,achangein

1 mediaortheabsenceofanadditivefromtheATCCrecommendedmediamayaffectrecovery,growthand/or
functionofthisproduct.Ifanalternativemediumformulationisused,theATCCwarrantyforviabilityisno
longervalid.

IntendedUse
Disclaimers

Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.
CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Saccharomycescerevisiae
(ATCC204508)
Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproductsheet,
ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureand
patentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeen
confirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.ATCC
isnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.Whilereasonableeffort
ismadetoinsureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfrom
themisidentificationormisrepresentationofsuchmaterials.
PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2016.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[08/04]

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org

800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

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