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    100% Percoll: Bahan Bahan Yang Harus Disiapkan

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    Pro Kartu
    The document provides instructions for making different solutions used in cell culture and laboratory experiments. It lists the components and quantities needed to make 100% and 50% Percoll solutions, BWW working stock solutions in 100ml and 200ml volumes, and a 50% Percoll solution. Reagents and materials needed for experiments involving cell separation, protein extraction, gel electrophoresis, and western blotting are also listed.

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    100% Percoll: Bahan Bahan Yang Harus Disiapkan

    Uploaded by

    Pro Kartu
    35 views2 pages
    The document provides instructions for making different solutions used in cell culture and laboratory experiments. It lists the components and quantities needed to make 100% and 50% Percoll solutions, BWW working stock solutions in 100ml and 200ml volumes, and a 50% Percoll solution. Reagents and materials needed for experiments involving cell separation, protein extraction, gel electrophoresis, and western blotting are also listed.

    Original Description:

    membuat larutan

    Original Title

    Solution Penelitian

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    The document provides instructions for making different solutions used in cell culture and laboratory experiments. It lists the components and quantities needed to make 100% and 50% Percoll solutions, BWW working stock solutions in 100ml and 200ml volumes, and a 50% Percoll solution. Reagents and materials needed for experiments involving cell separation, protein extraction, gel electrophoresis, and western blotting are also listed.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as docx, pdf, or txt
    35 views2 pages

    100% Percoll: Bahan Bahan Yang Harus Disiapkan

    Uploaded by

    Pro Kartu
    The document provides instructions for making different solutions used in cell culture and laboratory experiments. It lists the components and quantities needed to make 100% and 50% Percoll solutions, BWW working stock solutions in 100ml and 200ml volumes, and a 50% Percoll solution. Reagents and materials needed for experiments involving cell separation, protein extraction, gel electrophoresis, and western blotting are also listed.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    of 2

    100% Percoll

    (50ml) (100ml)

    0.05g (50mg) Polyvinyl Alcohol (PVA) 0.1g (100mg) Polyvinyl Alcohol (PVA)
    0.105g (105mg) Sodium Hydrogen Carbonate 0.21g (210mg) Sodium Hydrogen Carbonate
    (Sodium Bicarbonate-NaHCO3) (Sodium Bicarbonate-NaHCO3)
    5ml 10x HAMS/F10 Media 10ml 10x HAMS/F10 Media
    45ml Percoll 90ml Percoll
    0.025ml (25l) Sodium Pyruvate stock 0.05ml (50l) Sodium Pyruvate stock
    0.195ml (195l) Sodium Lactate (NaC3H5O3) 0.390ml (390l) Sodium Lactate (NaC3H5O3)

    50% Percoll (3 ml)

    1.5ml 100% Percoll


    1.5ml BWW Working Stock

    BWW Working Stock

    (100 ml) (200 ml)

    0.21g (210mg) Sodium Hydrogen Carbonate 0.42g (420mg) Sodium Hydrogen Carbonate
    0.1g (100mg) D-Glucose 0.2g (200mg) D-Glucose
    0.1g (100mg) Polyvinyl Alcohol (PVA) 0.2g (200mg) Polyvinyl Alcohol (PVA)
    0.05ml (50l) Sodium Pyruvate stock 0.1ml (100l) Sodium Pyruvate stock
    1ml Pen/strep 2ml Pen/strep
    2ml HEPES 4ml HEPES
    0.37ml (370l) Sodium Lactate (NaC3H5O3) 0.740ml (740l) Sodium Lactate (NaC3H5O3)
    Make up to 100ml with BWW stock Make up to 200ml with BWW stock
    Take 20l to CHECK THE OSMALARITY Take 20l to CHECK THE OSMALARITY should
    should be 290-310 osmoles be 290-310 osmoles
    Store @37C until required or at 4C for up to 2 days Store @37C until required or at 4C for up to 2
    days

    31. APS
    32. ddH2O
    33. Bromophenolblue
    34. Glycerine
    35. Beta mercaptethanol
    36. Protease inhibitor
    37. Fruktose
    38. Sukrose
    39. Protein marker
    40. Mouse anti IgG FITC conjugated
    41. Methanol
    42. Ethanol
    43. Acetic acid
    Bahan bahan yang harus disiapkan : 44.
    45.
    46.
    47.
    48.
    49.
    1. Percoll 50-70%
    2. BWW
    3. Larutan HO
    4. Eosin Y
    5. SDS extraction buffer
    6. 10% SDS Page
    7. 3% BSA
    8. TBST
    9. 2% paraformaldehyde (PFA)
    10. PBS
    11. 0.1 triton X-100
    12. Antiphosphotyrosine antibody
    13. FITC
    14. Poly-L-Lysine
    15. Mounting Media
    16. HEPES
    17. Hamis F10 medium
    18. Sodium Chloride
    19. Sodium citrate
    20. Potasium chloride
    21. Calcium chloride dihydrate
    22. Magnesium sulphate
    23. Mili-Q-H2O
    24. Sodium bicrbonate
    25. Citric acid
    26. 40% Acrilamid
    27. Trisbase
    28. Glycine
    29. Twen20
    30. Temed

    Alat yang dibutuhkan :

    1. Latex glove 11. ECL plus, western blot


    2. Eppendorf tube 12. Membran PVDF
    3. Gelas penutup bulat 13. Kodak film (100 sheet)
    4. Falcon tube 50ml 14. Developer (5 lt)
    5. Falcon tube 15ml 15. Fixer
    6. Slide mitul preparat 16. Mikroskop
    7. Blue tip 17.
    8. Yellow tip 18.
    9. Micro tip (0.5-10l) 19.
    10. Transfer pipet 20.

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