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  • Total Flavonoid BG Denny

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    The document presents the results of a standard curve calibration for quercetin. It describes the linear regression analysis that was conducted to develop the equation relating absorbance (Y) to concentration (X). The equation derived is Y = 0.032792x - 0.04075. Several treatments are then described where absorbance readings were used to calculate flavonoid concentrations and content based on the calibration equation. Average flavonoid contents are reported for each treatment.

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    Perhitungan total flavonoid

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    Total Flavonoid Bg Denny

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    The document presents the results of a standard curve calibration for quercetin. It describes the linear regression analysis that was conducted to develop the equation relating absorbance (Y) to concentration (X). The equation derived is Y = 0.032792x - 0.04075. Several treatments are then described where absorbance readings were used to calculate flavonoid concentrations and content based on the calibration equation. Average flavonoid contents are reported for each treatment.

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    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
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    Download as xlsx, pdf, or txt
    8 views13 pages

    Total Flavonoid BG Denny

    Uploaded by

    Be Graph
    The document presents the results of a standard curve calibration for quercetin. It describes the linear regression analysis that was conducted to develop the equation relating absorbance (Y) to concentration (X). The equation derived is Y = 0.032792x - 0.04075. Several treatments are then described where absorbance readings were used to calculate flavonoid concentrations and content based on the calibration equation. Average flavonoid contents are reported for each treatment.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    of 13

    Kurva kalibrasi standar kuersetin

    X Y XY X2 Y2
    0 0 0 0 0
    6.25 0.266 1.6625 39.0625 0.070756
    12.5 0.473 5.9125 156.2500 0.223729
    25 0.873 21.8250 625 0.762129
    50 1.666 83.3000 2,500 2.775556

    93.7500 3.2780 112.7000 3320.3125 3.8322


    JUMLAH
    RATA-RATA 18.75 0.6556 22.54 664.0625 0.766434

    r= 51.2375
    AKAR DARI 2629.864375
    r= 0.99922

    r=
    = 0,995256
    a= 51.2375
    1562.5
    a= 0.032792

    b= Y - AX
    b= 0.04075

    JADI, Y = 0.032792x - 0.04075


    JADI, Y = 0.032792x - 0.04075

    Perlakuan I:
    Abs = 0,244 0.244
    1.932 = 0.02856X + 0.10354
    x = (1.932 + 0.10354)/ 0.02856
    x= 6.19816 ppm
    Kadar flavonoid total 20.63987 mg/g QE

    % flavonoid total = 2.06399 %

    Perlakuan II:
    Abs = 0,245 0.245 20.63987
    1.930 = 0.02856X - 0.10354 20.74142
    x = (1.930 + 0.10354)/ 0.02856
    x= 6.22865 ppm
    Kadar flavonoid total 20.74142 mg/g QE

    % flavonoid total = 2.07414 %

    Perlakuan III:
    Abs = 0,244 0.244
    1.930 = 0.02856X - 0.10354
    x = (1.930 + 0.10354)/ 0.02856
    x= 8.68352 ppm
    Kadar flavonoid total 28.91612 mg/g QE

    % flavonoid total = 2.89161 %

    Perlakuan IV:
    Abs = 0,245 0.245
    1.930 = 0.02856X - 0.10354
    x = (1.930 + 0.10354)/ 0.02856
    x= 6.22865 ppm
    Kadar flavonoid total 20.74142 mg/g QE

    % flavonoid total = 2.07414 %


    Perlakuan V:
    Abs = 0,245 0.245
    1.930 = 0.02856X - 0.10354
    x = (1.930 + 0.10354)/ 0.02856
    x= 6.22865 ppm
    Kadar flavonoid total 20.74142 mg/g QE

    % flavonoid total = 2.07414 %


    a= 0.03279
    b= 0.04075
    JADI, Y = 0.032792x - 0.04075

    Perlakuan I:
    Abs = 1,007 1.007
    0.591 = 0.02856X - 0.10354
    x = (1.932 + 0.10354)/ 0.02856
    x= 29.46603 ppm
    Kadar flavonoid total 98.12187 mg/g QE

    % flavonoid total = 9.81219 %

    Perlakuan II:
    Abs = 1,007 1.007
    0.591 0.02856X - 0.10354
    x = (1.930 + 0.10354)/ 0.02856
    x= 29.46603 ppm
    Kadar flavonoid total 98.12187 mg/g QE

    % flavonoid total = 9.81219 %

    Perlakuan III:
    Abs = 1,007 1.007
    0.591 0.02856X - 0.10354
    x = (1.930 + 0.10354)/ 0.02856
    x= 29.4660283 ppm
    Kadar flavonoid total 98.121874238 mg/g QE

    % flavonoid total = 9.8121874238 %

    Perlakuan IV:
    Abs = 1,006 1.006
    0.590 = 0.02856X - 0.10354
    x = (1.930 + 0.10354)/ 0.02856
    x= 29.43553 ppm
    Kadar flavonoid total 98.02033 mg/g QE

    % flavonoid total = 9.80203 %


    Perlakuan V:
    Abs = 1,006 1.006
    0.591 0.02856X - 0.10354
    x = (1.930 + 0.10354)/ 0.02856
    x= 29.43553 ppm
    Kadar flavonoid total 98.02033 mg/g QE

    % flavonoid total = 9.80203 %


    a= 0.03279
    b= 0.04075
    JADI, Y = 0.032792x - 0.04075

    Perlakuan I:
    Abs = 1,152 1.152
    0.591 = 0.02856X - 0.10354
    x = (1.932 + 0.10354)/ 0.02856
    x= 33.88784 ppm
    Kadar flavonoid total 112.84650 mg/g QE

    % flavonoid total = 11.28465 %

    Perlakuan II:
    Abs = 1,152 1.152
    0.591 0.02856X - 0.10354
    x = (1.930 + 0.10354)/ 0.02856
    x= 33.88784 ppm
    Kadar flavonoid total 112.84650 mg/g QE

    % flavonoid total = 11.28465 %

    Perlakuan III:
    Abs = 1,152 1.152
    0.591 0.02856X - 0.10354
    x = (1.930 + 0.10354)/ 0.02856
    x= 33.88784 ppm
    Kadar flavonoid total 112.84650 mg/g QE

    % flavonoid total = 11.28465 %

    Perlakuan IV:
    Abs = 1,151 1.151
    0.590 = 0.02856X - 0.10354
    x = (1.930 + 0.10354)/ 0.02856
    x= 33.85734 ppm
    Kadar flavonoid total 112.74495 mg/g QE

    % flavonoid total = 11.27450 %


    Perlakuan V:
    Abs = 1,152 1.152
    0.591 0.02856X - 0.10354
    x = (1.930 + 0.10354)/ 0.02856
    x= 33.88784 ppm
    Kadar flavonoid total 112.84650 mg/g QE

    % flavonoid total = 11.28465 %


    a= 0.03279
    b= 0.04075
    Rata-Rata
    Kadar Flavonoid
    Konsentrasi Kadar Flavonoid
    Larutan Uji Absorbansi Total (mg
    (ppm) Total (mg
    GAE/g)
    GAE/g)
    1.007 98.12187
    1.007 98.12187
    BINARA 300 1.007 98.12187 98.08125
    1.006 98.02033
    1.006 98.02033
    0.244 20.63987
    0.245 20.74142
    PM 300 0.244 20.63987 20.70080
    0.245 20.74142
    0.245 20.74142
    1.152 112.84650
    1.152 112.84650
    DAUN AFRIKA 300 1.152 112.84650 112.82619
    1.151 112.74495
    1.152 112.84650
    112.74495

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