Leptospirosis Questions 1

1. Transport medium for Leptospira includes which of the following to control growth of
other bacteria
a) 5-Fluoro uracil
b) Amphotericin
c) Cyclohexamide
d) Doxycycline
2. What media is used to grow Leptospira?
a) EMJH Media
b) L J Media
c) Mc Coy Agar
d) Muller Hinton Agar
3. What is the suitable temperature for leptospiral growth?
a) 15 to 18C
b) 32 to 35C
c) 28 to 30C
d) 10 to14 C
4. Which of the following test is most preferred in diagnosis of leptospirosis?
a) Serum tube agglutination test (STAT)
b) Microscopic agglutination test (MAT)
c) Rose Bengal Plate Test (RBPT)
d) Serum neutralization test (SNT)
5. Which of the following is associated with Leptospira isolation?
a) Ringers ring
b) Dingers ring
c) Singers ring
d) Fingers ring
6. Which microscope is best suited for Leptospira identification?
a) Dark field microscope
b) Inverted microscope
c) Dissection microscope
 d) All of the above
7. The sequential procedure in ELISA is
a) Reading with ELISA reader-Addition of serum-Addition of conjugate-Addition of
substrate
b) Addition of serum-Addition of conjugate-Addition of substrate- Reading with
ELISA reader
c) Reading with ELISA reader- Addition of conjugate-Addition of substrate-
Addition of serum
d) Reading with ELISA reader- Addition of substrate- Addition of conjugate-
Addition of serum
8. Which of the following is not required to carry out PCR?
a) Primers
b) Taq polymerase
c) Reverse trancriptase
d) dNTPs
9. Which of the following is not required to carry out Real time PCR?
a) Primers
b) Template DNA
c) UV transilluminator
d) PCR plate
10. Multiplex PCR requires?
a) One primer
b) Two primers
c) No primer
d) More than two primers