USOO8947656B2

(12) United States Patent (10) Patent N0.: US 8,947,656 B2

Cunningham (45) Date of Patent: Feb. 3, 2015

(54) SMARTPHONE BIOSENSOR (56) References Cited

(71) Applicant: The Board of Trustees of the U-S- PATENT DOCUMENTS
University of Illinois, Urbana, IL (US) 7,385,460 Bl 6/2008 et al.
(72) Inventor: Brian T. Cunningham, Champaign, IL 7479404 B2 1/2009 _Cunmngham et al'
(Us) (Continued)
(73) Assignee: The Board of Trustees of the FOREIGN PATENT DOCUMENTS
University of Illinois, Urbana, IL (US) W0 W02010045949 * 7/2010
(*) Notice: Subject to any disclaimer, the term of this OTHER PUBLICATIONS
patent is extended or adjusted under 35 _ _
U_S_C_ 15403) by 0 days_ Abravaya et al., Molecular Beacons as Diagnostic Tools: Technol
ogy and Applications, Clin. Chem. Lab. Med., 2003, pp. 468-474,
(21) Appl. N0.: 13/801,978 M 41, Issue 4
(Continued)
(22) Filed: Mar. 13, 2013
_ _ _ Primary Examiner * Melanie Y Brown
(65) Pnor PUbhcatlon Data Assistant Examiner * Rebecca Martinez
Us 2014/0193839 A1 Ju1_ 10, 2014 (74) Attorney, Agent, or Firm * McDonnell Boehnen
Hulbert & Berghoff LLP

Related US. Application Data (57) ABSTRACT

- - - - A mobile computing device that includes an image sensor
(60) Provmonal apphcanon NO' 61/748903 ?led on Jan' may be used to detect the result of a biomolecular assay. The
4, 2013. . . .
biomolecular assay may be performed in an optical assay
medium that provides an optical output in response to light
(51) Int. Cl. . . . . . .
from a light source, With the optical output indicating result.
G01] 3/28 (2006.01) ~ ~ ~
G01] 3/44 2006 01 A wavelength-dispersive element may be used to disperse the
( ' ) optical output into spatially-separated wavelength compo
G01N 21/63 (200601) nents. The mobile computing device may be positioned rela
(52) U-s- Cl- tive to the wavelength-dispersive element such that different
CPC G0113/28 (2013-01); G01] 3/44 (201301); wavelength components are received at different locations on
G0113/4406 (201301); G01N21/63 (2013-01) the image sensor. With the mobile computing device posi
USPC ~~~~ ~~ 356/300; 356/301; 435/2887; 422/8205 tioned in this way, the image sensor may be used to obtain one
(58) Field of ClaSSi?catiOIl SeaICh or more images that include the separated wavelength com
CPC G01N 15/0205; G01N 23/06; G01N 23/02; ponents of the optical output. A wavelength spectrum of the
G01N 33/50; G01N 33/5302; G01N 33/581; optical output may be determined from the one or more
G01N 33/54366; B01L 2300/06; B01L images, and the result may be determined from the wave
2219/0097; B01L 2219/00689; G02B 13/001; length spectrum.
G06K 9/00127; G06F 19/30
See application ?le for complete search history. 11 Claims, 18 Drawing Sheets

426 '

400 \ 440 DOOR

432
ROTATABLE LINEAR
POLARIZING FILTER
408 /
SAMPLE
CHAMBER

424 ~ 418
LASER DIFFRACTION GRATING
430
EMTSSION FILTER

-== MULTTMODE FIBER

IE! COLLIMATING LENS

402
SMART
PHONE
 US 8,947,656 B2
Page 2
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 US 8,947,656 B2
Page 3

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US. Patent Feb. 3, 2015 Sheet 1 0f 18 US 8,947,656 B2

FIG. 1A

/
,<_K
SPECTROMETER g
1%PE
\ WAVELENGTH (nm)
TRANSMDTED
LIGHT

PHOTONlC CRYSTAL

CDLUMATED REFLECTED
BROADBAND NARROW BAND OF
UGHT WAVELENGTHS

FIG. 1B
US. Patent Feb. 3, 2015 Sheet 2 0f 18 US 8,947,656 B2

100 PHOTONIC
\ CRYSTAL

FIG. 2A
US. Patent Feb. 3, 2015 Sheet 3 0f 18 US 8,947,656 B2

FIG. ZB
US. Patent Feb. 3, 2015 Sheet 4 0f 18 US 8,947,656 B2

f LJI
.1
US. Patent Feb. 3, 2015 Sheet 5 0f 18 US 8,947,656 B2

FIG. SB
US. Patent Feb. 3, 2015 Sheet 6 0f 18 US 8,947,656 B2

FIG. 4A

100

5 80
E
g;
E 00
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@ 40
;; MEASUREDWITH
<2: , SMARTPHONE
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1100 1200 1000 1400 1500

PIXEL (NDEX

FIG. 4B
Patent FEb- 3, 2015 Sheet 7 0f 18 US 8,947,656 B2

304
M0
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CAMERA
\ PROCESSOR
COMMOMOAMOM
{NTERFACE _
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LED
PROGRAM DLSPLAY _\
/ // LNSTRUCTLONS 306
312 320/
322\
\\ DATA
USER INTERFACE \308
318\
\ MEMORY
3M CONTROLLER

FIG. 5
US. Patent Feb. 3, 2015 Sheet 8 0f 18 US 8,947,656 B2

Carrier '3 1:27 AM I! Carrier 7 1:29 AM .3 Carrier FF 1:35 AM I)

IBiosensor brainwaan New Experiment (Biwean Settings

Exp '0 ThreshoId

1 Images perTriaI I
Polarization span E]
New Experimem LED Lamp CaIibratIon Lambda [1]

Load Expenment Experiment Type PC + Samps Fined Points

Semngs PC Res Wavelegth Finer

SampIe Name SIOZ
NanoSensors Group

FIG. 6A FIG. 68 FIG. 6C

Carrier ? 1:39 AM I: Carrier ? 1:40 AM .7

Spectrum
Normalized Intensity
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IETrfansi%mcIe]ndy mD

WaveIengIh hm]
PC PWV: 565.08 Shift; 4-71
Sample PWV: 569.79

FIG. 6D FIG. 6E
US. Patent Feb. 3, 2015 Sheet 9 0f 18 US 8,947,656 B2

100 . i 1

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80 -- SPECTROMETER "
,2, "? SPECTROMETER
3 -~-- SPECTROMETER
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z //
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20 -

m -213
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500 550 600 650

WAVELENGTH {nm}

FIG. 7
US. Patent Feb. 3, 2015 Sheet 10 0f 18 US 8,947,656 B2

80

2;
L? 60
g
E
Z
40
.55
20 g
m- BARE P012

BARE PC 13
540 560 580 600
WAVELENGTH {mm}

FIG. 8
US. Patent Feb. 3, 2015 Sheet 11 0118 US 8,947,656 B2

100

80

ii;LZU
g 60 ,
Lg / _ BARE PC
% ;' PC +20nm $102
<12 :
c?) 40" 1 ..
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E 1'
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565177 nm ~570185 nm

540 560 580 600 620

WAVELENGTH {mm}

FIG. 9
US. Patent Feb. 3, 2015 Sheet 12 0f 18 US 8,947,656 B2

100 ' .

80 -

%
g 60 -

g _ BARE PC
5 ---- PC +15nm PPL
<2 40 -
E
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20 - 565.02 mm

520 540 560 580 600

WAVELENGTH {nm}

FIG. 10
US. Patent Feb. 3, 2015 Sheet 13 0f 18 US 8,947,656 B2

[nm]vavSHIFT
05 -

0 260 460 660 860 1600

CONCENTRATION [mm]

FIG. 11
US. Patent Feb. 3, 2015 Sheet 14 0f 18 US 8,947,656 B2

MLRROR PHOTONLC CRYSTAL

316
31s
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LENS
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300 CAMERA LED
PHONE
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US. Patent Feb. 3, 2015 Sheet 15 0f 18 US 8,947,656 B2

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US. Patent Feb.3,2015 Sheet160f18 US 8,947,656 B2

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