Nghin cu tc dng chng vim cp trn m hnh thc nghim Winter (gy

ph bn chn chut bng carrageenin) vi thin nin kin

S dng chut cng, c 2 ging, trng lng khong 100-150g, chia ngu
nhin thnh cc l, mi l 9 con [41].
- L 1 (l chng trng): ung dung dch nc mui sinh l.
- L 2 (l chng dng): ung Indomethacin vo thi im 30 pht trc
khi gy vim.
- L 3 (l th): ung dch chit Xng s bng kim cong u t vo thi
im 60pht, 30 pht trc gy vim,30 pht sau gy vim.
Chut c ung thuc trc khi gy vim 1 gi. Tin hnh gy vim bng
cch tim 0,1ml carrageenin 1% v gan bn chn sau, bn phi ca chut.
o th tch bn chn chut (n khp c chn) vo cc thi im: trc khi
gy vim, sau khi gy vim (thi im chn chut ph to nht).
Mc tng th tch chn ca tng chut c tnh theo cng thc (1) ca
Fontaine:
VtVo
(%) = 100 (1)
Vo

Trong : Vo: th tch chn chut trc khi gy vim

Vt: th tch chn chut sau khi gy vim
X%: tng th tch chn chut
Sau tnh trung bnh t l % tng th tch chn ca cc chut trong mi
l.
T l % c ch ph ca l th so vi l chng c tnh theo cng thc (2):
Xc.tb% Xt.tb%
% = 100 (2)
Xc.tb%

Trong :
I%: tl % gim mc ph bn chn chut.
Xc.tb%: trung bnh t l % tng th tch chn chut l chng.
 Xt.tb%: trung bnh t l % tng th tch chn chut l th.

Tc dng gim au ca Thin nin kin theo m hnh nh ti gi lc ny

nh.

Tc dng chng oxy ha cu H th :

Ha cht: 1,1-diphenyl -2-picrylhydrazyl (DPPH), methanol, acid ascorbic t tiu

chun phn tch.

Thit b: My o quang UV bc sng 517 nm Aligent technologies Cary 60 UV-Vis

Phng php nghin cu

Nguyn tc th tc dng:

Hp cht 1,1-diphenyl-2-picrylhydrazyl (DPPH) c kh nng to ra gc t do bn

trong dung dch MeOH bo ha. Khi cho cc cht th nghim vo dung dch ny, nu
cht c kh nng qut cc gc t do s c kh nng lm gim cng hp th nh sng
ca cc gc t do DPPH. Kh nng chng xy ha c nh gi thng qua gi tr hp
th nh sng ca dung dch th nghim so vi cht i chng, khi o bc sng 517
nm [3], [4].

Cch tin hnh thc hin:

+ Cn chnh xc khi lng (mg) ca phn on dch chit A, B v acid ascorbic, sau
ha tan trong 1 ml methanol, lc b phn khng tan ca dch chit. Pha long c
cc dung dch c nng nh hn.

+ Ha tan 4,3 mg DPPH (1,1-diphenyl-2-picrylhydrazyl) trong 3,3 ml methanol trong

ng nghim c bc bi giy nhm trnh nh sng.

+ Mu trng: 3150 l MeOH v o hp th ti bc sng 517 nm.

+ Thm 150 l dung dch DPPH vo 3 ml methanol v o hp th ngay lp tc ti

bc sng 517 nm (mu i chng).

+100 l mu th + 150 l DPPH + 2,9 ml methanol (mu th)

 +100 l cht chng dng (Acid ascorbic) + 150 l DPPH + 2,9 ml methanol (mu
chng dng)

+ o hp th ti bc sng 517 nm sau 15 min.

Tt c cc xt nghim c lp li 3 ln. Mu trng dng methanol.

Cng thc tnh kt qu:

(Ac Ao) (At Ao) Ac At

% AOA = x 100 = x 100
Ac Ao Ac Ao

Trong : AOA l hot tnh chng oxy ha (Antioxidative activity)

Ac : hp thu ca mu chng

At : hp thu ca mu th

Ao : hp thu ca mu trng

Tc dng chng xy ho ca dch chit c so snh vi cht chun dng l acid

ascorbic.

2.2.3. Phng php x l s liu

Cc s liu thc nghim c x l thng k theo phng php thng k
sinh hc, s dng cng c Data analysis ca Microsoft Excel. Kt qu th nghim
c biu th bng tr s trung bnh cng/ tr sai s chun (MSE). nh gi, so
snh gia cc l th nghim bng phng php thng k s dng chun t-Student
[8].