Scribd Logo
Upload

Welcome to Scribd!

  • Atcc Nms Medium 1306

    Uploaded by

    Victor J Torres
    31 views1 page
    This document provides the recipe and preparation instructions for ATCC medium 1306, which is a nitrate mineral salts medium used for growing microorganisms. It lists the ingredients and amounts needed for the base medium, including magnesium sulfate, calcium chloride, potassium nitrate, dipotassium phosphate, and sodium phosphate. It also provides recipes for a chelated iron solution and trace element solution that are added to the base medium in specified volumes. The completed medium is adjusted to pH 6.8, autoclaved, and then used to culture microbes.

    Original Description:

    Culture medium 1306

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    This document provides the recipe and preparation instructions for ATCC medium 1306, which is a nitrate mineral salts medium used for growing microorganisms. It lists the ingredients and amounts needed for the base medium, including magnesium sulfate, calcium chloride, potassium nitrate, dipotassium phosphate, and sodium phosphate. It also provides recipes for a chelated iron solution and trace element solution that are added to the base medium in specified volumes. The completed medium is adjusted to pH 6.8, autoclaved, and then used to culture microbes.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    31 views1 page

    Atcc Nms Medium 1306

    Uploaded by

    Victor J Torres
    This document provides the recipe and preparation instructions for ATCC medium 1306, which is a nitrate mineral salts medium used for growing microorganisms. It lists the ingredients and amounts needed for the base medium, including magnesium sulfate, calcium chloride, potassium nitrate, dipotassium phosphate, and sodium phosphate. It also provides recipes for a chelated iron solution and trace element solution that are added to the base medium in specified volumes. The completed medium is adjusted to pH 6.8, autoclaved, and then used to culture microbes.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    Download as pdf or txt
    of 1

    ATCC medium: 1306 Nitrate mineral salts medium (NMS)

    MgSO4 . 7H2O .............................1.0 g


    CaCl2 . 6H2O .............................0.20 g
    Chelated Iron Solution (see below).......2.0 ml
    KNO3 .....................................1.0 g
    Trace Element Solution (see below).......0.5 ml
    KH2PO4 ...................................0.272 g
    Na2HPO4 . 12H2O...........................0.717 g
    Purified Agar (e.g., Oxoid L28).........12.5 g
    Distilled deionized water................1.0 L

    Adjust pH to 6.8. Autoclave at 121C for 15 minutes.

    Chelated Iron Solution:


    Ferric (III) ammonium citrate*...........0.1 g
    EDTA, sodium salt........................0.2 g
    HCl (concentrated).......................0.3 ml
    Distilled deionized water..............100.0 ml

    *0.05 g of Ferric (III) chloride may be substituted.

    Use 2.0 ml of this chelated iron solution per liter of final medium.

    Trace Element Solution:


    EDTA.................500.0 mg
    FeSO4 . 7H2O .........200.0 mg
    ZnSO4 . 7H2O ..........10.0 mg
    MnCl2 . 4H2O ...........3.0 mg
    H3BO3 .................30.0 mg
    CoCl2 . 6H2O ..........20.0 mg
    CaCl2 . 2H2O ...........1.0 mg
    NiCl2 . 6H2O ...........2.0 mg
    Na2MoO4 . 2H2O..........3.0 mg
    Distilled water........1.0 L

    Autoclave at 121C for 15 minutes.

    You might also like