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Respiration
Respiration
6
HKALE 1994 Biology Paper II......................................................................................7
HKALE 1996 Biology Paper I.......................................................................................8
HKALE 1994 Biology Paper I.....................................................................................21
HKALE 1994 Biology Paper II....................................................................................22
HKALE 1996 Biology Paper I.....................................................................................24
Questions
HKALE 1989 Biology Paper II
4. (a) Define the term respiratory quotient. (2 marks)
(b) What information about the metabolism of an organism can be deduced from the
values of the respiratory quotient ? (4 marks)
(c) Give an account of the various means by which oxygen and carbon dioxide are
transported in the blood of a mammal. Indicate also their relative importance to the
total carrying capacity of blood for oxygen and carbon dioxide. (7 marks)
(d) Give an account of the factors that exist under physiological conditions to favour
(i) the release of oxygen from blood at the level of the tissue, and
(ii) the release of carbon dioxide from blood in the lungs. (7 marks)
Suggested Solution
HKALE 1990 Biology Paper II
1. (a) What is oxygen debt ? Explain its physiological significance. (5 marks)
(c) Explain why aquatic ectotherms using gills for gaseous exchange may be more
affected than terrestrial ectotherms by the same increase in surrounding
temperature. (2 marks)
(d) Describe how a change in the concentrations of carbon dioxide and oxygen in the
inspired air can affect the rate and depth of breathing in man. (4 marks)
(e) With reference to the graph below, explain the relative positions of the three
oxygen dissociation curves in relation to the size of the respective animals. (5
marks)
Suggested Solution
HKALE 1991 Biology Paper I
4. By means of a flow diagram, briefly outline the three main stages of aerobic respiration
with a carbohydrate substrate. Annotate your diagram to show the essential features of
each of the three main stages.
(N.B. Chemical formulae of individual compounds are NOT required .) (7 marks)
Suggested Solution
HKALE 1992 Biology Paper I
10. The ability of cells to synthesize ATP from ADP and inorganic phosphate is coupled with
the oxidation of substrates by oxygen.
(a) In an experiment to study the effect of potassium cyanide (KCN) on the respiration
of yeast cells, 4 tubes (with contents as shown in the table below) were prepared
and the corresponding rates of oxygen uptake were measured.
(i) Calculate the final concentrations (in moles per litre) of the following
substances in tube 3 :
(1) glucose
(2) KCN (3 marks)
(ii) Why was respiration observed in tube 1 even in the absence of added
glucose ? What was the result of adding glucose to tube 2 ? (2 marks)
(iii) What can you conclude about the effect of KCN on the respiration rate of
yeast cells ?
(2 marks)
(b) Mitochondria were isolated from the yeast cells and incubated in an isotonic buffer
containing a respiratory substrate, ADP and inorganic phosphate. The amount of
oxygen uptake and the loss of substrate and inorganic phosphate were measured.
(iii) How would the relationship in (ii) differ from those when glucose is oxidized in
intact cells under aerobic conditions ? Explain your answer. (4 marks)
Suggested Solution
HKALE 1994 Biology Paper I
9. With the aid of an oxygen dissociation curve, explain how the relationship between
oxygen tension and haemoglobin saturation facilitates oxygen uptake at the respiratory
surface and oxygen release in the tissues. (6 marks)
Suggested Solution
HKALE 1994 Biology Paper II
2. (a) Describe the three stages of cellular respiration for carbohydrate metabolism. (10
marks)
(b) Compare and contrast the products of the metabolic process in (a) in the presence
and absence of free oxygen. (3 marks)
(c) What is the role of ATP ? Describe the part ATP plays in three other named
metabolic processes and cite an example for each process. (5 marks)
(d) Explain why protein is less efficient for energy production when compared with (i)
lipid ; and (ii) carbohydrate. (2 marks)
Suggested Solution
HKALE 1996 Biology Paper I
4. The following shows the initial set-up of an experiment performed at room temperature.
Readings were taken at specific time intervals.
Suggested Solution
HKALE 1997 Biology Paper I
11.
The figure above shows an experimental set-up used to measure the oxygen
consumption rates of animals at room temperature A mouse was placed into the
respiratory chamber A syringe was inserted into the inlet hose to inject 10 mL of air into
the respiratory chamber The clamp was then closed and the time was noted; this was
the starting time of the experiment When the fluid levels in the manometer arms were
the same, the time was again noted; this was the ending time. The experiment was then
repeated using several grasshoppers A set of the experimental data is presented in the
table below:
Mouse Grasshopper
Body weight 20 g 5g
(a) At the starting time of the experiment, what happened to the fluid levels in the two
arms of the manometer ? Account for this observation. (1 marks)
(b) Account for the fluid levels in the manometer arms at the ending time of the
experiment. (3 marks)
(c) Calculate the oxygen consumption rates (mL O 2 h-1g-1) of the mouse and the
grasshoppers.
(3 marks)
(d) What is the function of the water bath ? State another precaution for this
experiment. (2 marts)
(e) How would the oxygen consumption rates of the mouse and the grasshoppers
change if the temperature in the respiratory chamber dropped from 25 to 5 ?
Explain your answer.
(3 marks)
Total :13 marks
Suggested Solution
HKALE 1997 Biology Paper II
1. (a) Explain the role of adenosine triphosphate (ATP) in cellular metabolism. (3 marks)
(c) List two animal cell types and one plant cell type where abundant ATP is required.
How is the ATP requirement related to the functions of these cells ? (3 marks)
Suggested Solution
HKALE 1998 Biology Paper II
5. Give a comparative account on how the chloroplast and mitochondrion process energy
and discuss the inter-relationship between these two organelles in cellular metabolism.
(20 marks)
Suggested Solution
HKALE 1989 Biology Paper II
Volume of carbon dioxide produced
4. (a) Respiratory quotient (R.Q.) =
volume of oxygen consumed
1
during the same period of time,
under a steady state condition
(2)
(c) Oxygen :
(i) In combination with ferrous ions of the heme groups of
haemoglobin to form oxyhaemoglobin (95%),
1
one molecule of haemoglobin when fully oxygenated carries four
molecules of oxygen
(ii) In physical solution as dissolved gas (5%)
1
Correct order of relative importance
(2)
Carbon dioxide :
(i) As bicarbonate ions (85%)
carbon dioxide diffuses into RBC and combines with water to
formcarbonic acid, catalyzed by carbonic anhydrase present
in RBC.
haemoglobin (deoxygenated) accepts hydrogen ions from
carbonic acid, leaving behind bicarbonate ions.
2
(b) An ectotherm may not be able to regulate its body temperature which
increases with an increase in ambient temperature.
1
With a 10C rise in body temperature, the metabolic rate (rate of
enzymic reactions) and
1
hence the respiration rate will increase / double according
1
to the Q10 rule / explanation of increasing temperature on rate of
enzymic reaction.
1
(4)
(d) The rate and depth of breathing are controlled by a respiratory centre in
the medulls,
1
which is responsive directly or indirectly (through chemoreceptors : the
aortic and carotid bodies in the walls of major arteries) to changes in
CO2, H+ and O2 concentrations in blood.
1
An increase in the concentration of CO2 and H+ in the blood stimulates
the respiratory centre which in turn, increases depth of breathing (and
vice versa)
1
Lack of oxygen stimulates chemoreceptors in carotid and aortic body
and stimulates respiration. Oxygen receptors however, are sensitive
only to large changes in blood PO 2 (<70 mm Hg) and is little affected by
slight changes of oxygen in blood.
1
Since oxygen in blood PCO 2 and PO2 normally are proportional to one
another, breathing is generally regulated by CO2 in blood.
(4)
(e) The smaller the animal, the farther its oxygen dissociation curve-is
shifted to the right.
1
The haemoglobin of small animals therefore unloads more of its oxygen
at any given pressure than does the haemoglobin of a larger animal.
1
Heat loss from an endotherm is proportional to its body surface area.
The smaller the animal, the larger is its S.A. / V ratio and hence its rate
of heat loss
1
In order to compensate for the higher rate of heat loss to maintain a
constant body temperature, the smaller animal needs to have a higher
metabolic rate
1
The shifting of the curve to the right allows small animals to obtain more
oxygen to sustain their higher metabolic rates at any given oxygen
partial pressure. (NOTE : partial pressure of oxygen in air and the lung
is normally higher than 100 mm Hg, and animals of all size should have
their haemoglobin fully saturated with oxygen at such partial pressure)
1
(5)
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HKALE 1991 Biology Paper I
(Glucose)
4. (C4) SUGARS
Phosphorylation of glucose
which eventually splits into
()
() GLYCOLYSIS
2 trioses (some ATP
production)
() PYRUVIC ACID
loss of molecule of CO2 to
()
produce a 2C molecule
() 2C compound
()
(progressive transfer of
ELECTRON ()
O2 TRANSPORT electrons from one
() ATP
(as the final CHAIN (system) () carrier to another, with
electron the energy released to
acceptor)
form ATP.)
H2O ()
(7)
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HKALE 1992 Biology Paper I
10. (a) (i) Either one of the following formulae can be used :
(1) M1V1 = M2V2
(M 2 V2 )
M1 =
V1
Amount of
(2) dissolved substances
Final concentration =
Final volume
2M 0.01 ml
Glucose concentration = = 0.01M / 9.9 10-3M
(2 + 0.02) ml
1
0.1M 0.01 ml
KCN concentration = = 0.0005 M / 4.95 10-4M
(2 + 0.02) ml
1
N.B. For correct answers (1) each, correct equation but wrong answer (1)
each.
14
12
O xyg e n
s u b st ra t e
Oxygen uptake OR Loss of substrate ( mole)
in o r g a n ic p h o s p h a t e
5 10
4 8
3 6
2 4
1 2
0 5 10 15 20 25 30 35 40 45
Tim e ( m in . )
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HKALE 1994 Biology Paper I
9.
(b) Similarities
Both aerobic and anaerobic respiration yield energy / ATP
Differences
More ATP is produced in aerobic respiration.
In anaerobic respiration (fermentation)
in glycolysis, pyruvate is not the end product. In many bacteria, fungi
and animal cells (), this anaerobic process may result in the formation
of lactate ().
+
In yeast and most plant cells (), pyruvate is broken down to ethanol ().
+
(3)
(c) ATP is hydrolyzed to ADP and P i, this process liberates energy for
energy consuming metabolic processes.
1
max. (20)
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HKALE 1996 Biology Paper I
4. (a) To find out the rate of anaerobic respiration () (rate of carbon dioxide
released or rate of fermentation) () of the yeast suspension.
(1)
correct formula. ()
(2)
(c) Any one
Glucose solution is boiled ()and cooled () (just mention cooled,
no mark) +
No air trap inside syringe initially (1)
Ensure that the set-up () is air-tight () (just mention set-up, no
mark) +
The yeast used is viable (1)
(no mark for constant temperature maintenance)
(if more than one precaution are given, mark the first precaution only)
(1)
(Q4 = 4 marks)
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HKALE 1997 Biology Paper I
11. (a) Start : fluid level on the left arm of manometer was higher () (accept
the reverse description for the right arm of the manometer)
(1)
Reason : air was injected and air pressure inside the whole system
increased (1)
10
(c) Mouse : Rate = = 3.33 mL O2 h-1 g-1
20 0.15
1
10
Grasshoppers : Rate = = 0.36 mL O2 h-1 g-1
5 5. 5
1
[formula (), correct data (), correct answer with unit ()]
(3)
(d) To stabilize the temperature during the course of the experiment (1).
1
Another precaution :
Allow the animal(s) to acclimatize to the temperature before starting the
experiment(1) / To make sure the apparatus is air tight.
1
(2)
(e) Mouse : rate increases ()
Grasshoppers : rate decreases ()
1
electron from NADH passes along ATP ()and NADPH () carrying the 6
the electron transport chain (1) where energy are used in the dark reaction to
members of the chain undergo oxidation- synthesize carbohydrate (1) / hexose /
reduction reactions releasing energy starch, thus chloroplast converts light
(1)to build up ATP () from ADP (). This energy to potential / chemical energy ()
is oxidative phosphorylation (). stored in food
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