Biurets Lab Test

In this chemical lab

testing we used the
biurets test to
determine whether or
not our control
substance of 5 water
droplets and 5 protein
droplets when mixed
with 10 droplets of
biuret's solution would
indicate a positive
testing situation, which
is indicated by the
chemical color change
to a light lilac as
represented by our
second test tube.
The biuret test is used
for detecting the
presence of peptide
bonds which are
present in proteins by
using Cu(II) ions to
form a violet colored
chelate complex with
peptide bonds.
Low peptide bonds will result in a pink hue, while a higher concentration of peptide bonds is
shown with a darker variant of color.