. Tetrazolium Testing Handbook for Agricultural Seeds.

Plate II Wheat.

Upper left:
All seeds germinable. Embryos completely stained.

Upper right:
First seed: Germinable. Embryo completely stained.
Second seed: Germinable. Radicle and lower part of scutellum unstained.
Third seed: Non-germinable. Radicle, lower part of scutellum and seminal root area
unstained.
Fourth seed: Germinable. Radicle unstained.

Lower left:
All seeds non-germinable. Radicles and plumules unstained.

Lower right:
First seed: Non-germinable. Embryo entirely unstained.
Second seed: Non-germinable. Embryo nearly all unstained.
Third seed: Non-germinable. Embryonic axis unstained.
Fourth seed: Non-germinable. Radicle and seminal root area unstained.
 Tetrazolium Testing Handbook for Agricultural Seeds.
Handbook on Seed Testing, AOSA.
Plate I Corn.

Upper left:
First seed ------ Germinable. Embryo completeley stained. Note dotted appearance of scutellum
Second seed - Germinable. Embryo completely stained.
Third seed ----- Germinable. Slight deterioration of embryonic axis.

Upper right:
First seed ----- Germinable. Moderate deterioration of embryonic axis
Second seed - Germinable. Radicle unstained. Unstained areas in scutellum and mesocotyl. Plumule and
seminal roots are stained.
Third seed - ---Germinable. Radicle and upper and lower parts of scutellum unstained. The stained areas
represent the minimum area of the embryo required for normal germination.

Lower left:
First seed ------ Non-germinable. Radicle and seminal root area unstained.
Second seed - Non-germinable. Majority of embyro unstained.
Third seed ---- Non-germinable. Shoot unstained.

Lower right:
First seed ----- Non-germinable. Embryonic axis unstained.
Second seed - Non-germinable. Embryonic axis and upper and lower parts of scutellum unstained.
Third seed ---- Non-germinable. Embryo almost completely unstained.
 . Tetrazolium Testing Handbook for Agricultural Seeds.
Figure 6 wheat.
originally appeared in:
Delouche, J.C., T. W. Still, M. Raspet, and M. Lienhard. 1962. The tetrazolium test for seed viability.
Miss. Agric. Exp. Sta. Tech. Bull. 51.

Criteria for interpreting tetrazolium test results on wheat seed. Black areas indicate stained
, living tissue; white areas
represent unstained and dead
tissue.
1. Germinable. Entire embryo stained
bright red.
2.-5. Germinable. Extremities of
scutellum unstained.
6. Germinable. Extremities of scutellum,
radicle tip, and coleorhiza
unstained.
7. Non-germinable. More than three-
fourths of radicle unstained.
8. Non-germinable. Plumule unstained.
9. Non-germinable. Central portion of
scutellum and scutellar node unstained.
10. Non-germinable. Embryonic axis
unstained.
11. Non-germinable. Extremities of
scutellum and plumule tip unstained.
12. Non-germinable. Entire upper half
of embryo unstained.
13. Non-germinable. Scutellum
unstained.
14. Non-germinable. Scutellum, radicle,
and coleorhiza unstained.
15. Non-germinable. Stain very faint
pink.
16. Non-germinable. Entire embryo
unstained.