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Wizard Genomic Dna Purification Kit Quick Protocol PDF
Wizard Genomic Dna Purification Kit Quick Protocol PDF
2818MA11_9A
9. Centrifuge: Air-dry pellet.
Rehydrate DNA.
300l sample 13,00016,000 g* ; 1 minute
110ml sample 2,000 g ; 1 minute
10. Discard supernatant. Add 70% ethanol (same volume as isopropanol).
11. Centrifuge as in Step 9.
12. Aspirate the ethanol and air-dry the pellet (1015 minutes).
13. Rehydrate the DNA in the appropriate volume of DNA Rehydration Solution for
1 hour at 65C or overnight at 4C.
Isolation of Genomic DNA from Animal Tissue and Tissue Culture Cells
Prepare Tissues
Cells or tissue
Tissue Culture Cells: Centrifuge at 13,00016,000 g* for 10 seconds. with Nuclei
Wash the cell pellet with PBS, vortex and then add 600l of Nuclei Lysis Lysis Solution.
Solution and mix by pipetting.
Animal Tissue: Add 1020mg of fresh or thawed tissue to 600l of chilled
Nuclei Lysis Solution and homogenize for 10 seconds. Alternatively, use
Add RNase
1020mg of ground tissue. Incubate at 65C for 1530 minutes. Solution.
Mouse Tail: Add 600l of chilled EDTA/Nuclei Lysis Solution to 0.51cm of Incubate at 37C.
fresh or thawed mouse tail. Add 17.5l of 20mg/ml Proteinase K and incubate
overnight at 55C with gentle shaking.
Isolation of Genomic DNA from Gram Positive and Gram Negative Bacteria
Pellet Cells
Centrifuge 1ml of overnight culture for 2 minutes at 13,00016,000 g*. Gram +
Discard the supernatant. Pellet cells.
12. Rehydrate the DNA pellet in 100l of Rehydration Solution for 1 hour at 65C Rehydrate DNA.
or overnight at 4C.
2826MA11_9A
temperature 70% ethanol. 300l 600l
6. Centrifuge at 13,00016,000 g*. 2 minutes 1 minute Centrifuge.