No.359Eo, Kampucheakrom Blvd, Sangkat Phsar Depot III, Khan Toul Kork, Phnom Penh, Cambodia a Tel: (855)-23 884 889 Fax: (855)-23 882 889 10 m e H/P: 097 7555555, E-mi iomedphnompenh@yahoo.com THE LEADER IN MOLECULAR DIAGNOSIS IN CAMBODIA Biomed 1D | V 1022257 | BiomedNK ID H 1267 Patient’ s name SONY Age 41 Year Sex M Provenance DOCTEUR EXTERN Type of Sample Blood (Serum) Test request Quantitative PCR for HBV-DNA Date of receiving the sample 10/22/2017 Date of carrying out the test 10/23/2017 Investigator Brachnha, Tran The Huu Phuoe Equipment to do the realtime PCR The CFX96 from Biorad (USA) Method to do the real-time PCR * Taqman probe labeled with FAM to detect HBV-DNA. Taqman probe labeled with Hex to detect the internal control DNA that is extracted with the sample. * The quantitation of HBV-DNA is carried out based on the 3 standards: $1: 1000, $2: 10.000, and $3: 100.000 IU ( International per Unit ) that are real-time detected with the sample’ s DNA Cycle sequencing reaction Big-Dye 3.1 (USA) Sequencer ABI 3130 (USA) Analysis software SeqScape v. 2.5 (USA) Limit of detection 200 copies/ml or 50 IU/ml REPORT The viral load (copies/ml) = 17,120 copies/ml The viral load ( login) =1.7x4 login The viral load (IU/ml) = 2,675 IU/ml The genotype Comments Patient is currently infected with HBV (hepatitis B virus) with low copies or low IU Patient needs to show this report to the physician to receive the appropriate comment for situation of infection Phnom Penh City,23 Oct 2017 Technicial of the laboratory and analysis =e Professor A. BUTH SOKHAL