Pertemuan Ke : 1 Biologi Sel

Waktu : 7 & 8 Oktober 2016. Pukul 13.00-15.00

Dosen : Ellyta Aizar, SKp.M.Biomed
Iwan Rusdi, SKp.MNS
Diah Arum, Skep. Ns. M.Kep

Tujuan praktikum :
Mahasiswa mengetahui materi dasar penyusun sel dan fungsinya

Indikator penilaian :
Mahasiswa dapat :
1. Mendeskripsikan bagian-bagian/organella sel
2. Mengaitkan struktur spesifik organella sel dengan fungsinya

Metode pembelajaran :
Observasi dan diskusi
Media :
Lab Kering (video sel dan organella sel)

Aspek penilaian :
1. Sikap (observasi oleh pembimbing praktikum)
2. Pengetahuan (tes)
3. Ketrampilan (laporan praktikum lembar kerja mahasiswa)

Bioetical Focus :
Stem cells
(Mahasiswa secara mandiri mencari informasi / pengetahuan tentang perkembangan
teknologi stem cells dan aplikasinya dalam dunia kesehatan. Mahasiswa harus bisa
menemukan apa fokus bioetik terkait penerapan teknologi stem cells dalam dunia
kesehatan dan menuliskannya dalam laporan praktikum)

Daftar Pustaka
1.
 Lembar Kerja Praktikum Sel

No Struktur Sel dan Organella Sel Fungsi

SEL

&

OR
GA
NEL
LA
NYA
1. N
U
C
L
E
U
S
&
N
U
C
L
E
O
L
U
S
2.
RE
TI
KU
LUM
EN
DO
PLAS
MIC
Lysosomes are found, in various
numbers, in all cell types. They
are membrane vesicles
approximately 0.5 microns in
diameter. They contain several
hydrolytic enzymes (proteases,
nucleases, glycosidases, lipases,
and phosphatases). In healthy
cells they serve an important
digestive function. Lysosomes
are produced by the Golgi and
when they bud off the mature
face are called primary
lysosomes. In routine TEM
preparations they appear as
homogeneous, electron
translucent vesicles (Lysosome
1). When a primary lysosome
fuses with a vesicle containing
unwanted material it is now
called a secondary lysosome. As
the source of unwanted
material differs, so does the
morphology of a secondary
lysosome. In general, the
homogeneous contents are
replaced by the now apparent
unwanted material (i.e.
bacteria, degenerated
organelle, etc). The hydrolytic
enzymes of the lysosome then
digests the material. Whatever
is left over is termed a residual
body (Lysosome 1).
Oxidation of nutrients and the
production of energy occurs
within the mitochondria. By
TEM, mitochondria are found to
be composed of two
membranes (outer and inner)
(Mitochondria). Both exhibit the
typical unit membrane
morphology. The outer
membrane plays a role in
controlling the movement of
substances into and out of the
mitochondria, uptake of
substrates, and release of ATP.
The inner membrane is
arranged in shelf-like folds
called cristae (Mitochondria 1).
(Note: Mitochondria are long
and often tortuous in shape.
They therefore have many
different appearances based on
the orientation of the section
through the structure.) The
enzymes associated with
electron transport and oxidative
phosphorylation are located on
the cristae, associated with
"toadstool" like structures, the
elementary particle. These
structures are visible in high
magnification micrographs of
negatively stained specimens
(Mitochondria 2).
.