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Flowchart
Flowchart
Flowchart
by Shishin Yamada
last updated: September 23, 1998
© "First Aid for the USMLE Step 1" by Appleton and Lange.
Bacterial Cocci
*Many thanks to Jason Collins for citing reference material for these charts
(from "The Microbiology Companion" book by ALERT and ORIENTATED publishers)
*KEY:
Catalase = Mix with 3% hydrogen peroxide. Catalase + if forms bubbles.
Coaguase = Check for coagulase enzyme. Coagulase + if plasma clots, clumps or forms precipitate.
Oxidase = Determines presence of cytochrome-c in bacteria. Oxidase + if paper turns dark purple quickly.
Alpha = alpha hemolysis (very small clear area of hemolysis around colonies on blood agar)
Beta = beta hemolysis (large clear area of hemolysis around colonies on blood agar)
Gamma = NO hemolysis (no signs of hemolysis around colonies on blood agar)
Fermentation of specific sugars means that they change the pH of the tube over 48 hours. Fermentation is + for
when the upper third of the tube turns yellow, while the bottom two-thirds remains red.
Gram Positive Rods
*Many thanks to Jason Collins for citing reference material for these charts
(from "The Microbiology Companion" book by ALERT and ORIENTATED publishers)
Bordetella pertusis Selective medium: Bordet-Gengou agar Whooping cough (only humans)