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Vinasses Fungal Production
Vinasses Fungal Production
Vinasses Fungal Production
7.1%
AQUATIC ANIMAL
PROTEIN MEALS
& LIPIDS
OILSEED LAND
MEALS, PULSES ANIMAL
PROTEIN
& OILS
40 MEALS &
essential FATS
nutrients
CEREALS
INCLUDING MICROBIAL
BY-PRODUCT FEED
MEALS INGREDIENTS
& OILS
200 µm
Why Fungi-Rhizopus microsporus var. oligosporus?
Source: Jennessen, J., Schürer, J., Olsson, J., Samson, R. A., and Dijksterhuis, J. 2008. Morphological characteristics of sporangiospores of the tempe fungus
Rhizopus oligosporus differentiate it from other taxa of the R. microsporus group. Mycological Research. 112, 547–563.
Fungal fermentation– biofuel residues
Dewatered product
Filtered water
~45% protein
pH 4.25 ± 0.32
pH:5.0
4 5 Temperature: 37oC
1
NaOH
23
Nutrient supplementation:
H2SO4
COD:N:P = 100:5:1
Aeration rates:
Foam trap
pH 5.0
pH controller
0.5, 1.0, 1.5, and 2.0
volumeair/volumeliq/min
1. Sampling port
2. Acid inlet
3. Base inlet
4. pH probe
5. Temperature probe
Air pump
Air filter
Rotameter
Fungal biomass yield
c
9
7
b
6
(gbiomass increase/ginitial biomass)
b
Fungal biomass yield
5
a
4
0
0.5 1.0 1.5 2.0
Fungal cultivation
at 1.5 vvm
Fungal cultivation
at 2.0 vvm (72 h)
Airlift vs. bubble column
10.0 a
Fungal biomass yield
b
8.0
b
a a a
6.0
a
4.0
2.0
0.0
0.5 1.0 1.5 2.0
Aeration rate (vvm)
Fungal cultivation for 72 h
a
~ 60% SCOD reduction
(gbiomass increase/ginitial biomass)
a
Fungal biomass yield
12
Fishmeal Soybean meal Vinasse-derived fungal biomass
(based on % protein)
10
SCOD (g/L)
SCOD (g/L)
20 20
15 15
10 10
5 5
0 0
0 1 2 3 0 1 2 3
Cultivation time (day) Cultivation time (day)
25 25
SCOD (g/L)
20 20
15 15
10 10
5 5
0 0
0 1 2 3 0 1 2 3
Cultivation time (day) Cultivation time (day)
50 40
45
Specific oxygen uptake rate
SCOD 35
(SOUR) (mg O2/g VSS-hr)
40
30
35
SCOD (g/L)
30 25
SOUR
25 20
20 15
15
10
10
5 5
0 0
0 8 16 24 48 72
Cultivation time (h)
Pilot-scale study
• Molasses, Taro wastes and
damages Papaya
bioconversion into fungal
protein
Lee Jakeway