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Antibacterial Activity of Wiregrass (Eleusine Indica) and Pandanus Specie Leaves Extract Against Escherichia Coli
Antibacterial Activity of Wiregrass (Eleusine Indica) and Pandanus Specie Leaves Extract Against Escherichia Coli
S C I E N C E INVESTIGATORY PROJECT
EMALYN F. PUNZALAN
Researcher
Emalyn F. Punzalan
Researcher
Abstract
Escherichia Coli.
control (chloramphenicol).
Tools:
Materials:
B. General Procedure
1. Extraction Procedure
specie leaves. The leaves are then pound using mortar and
4. Bio-assay Proper
the zone was then measured by means of a ruler and the average
of the data was taken from the data in the y and x axis of the
zone. The data were plotted in the tabulated form and treated
to Escherichia coli.
C. Procedural Design
Preparation of
Treatment C: 50 %( PANDANUS)
and 50% (BAKONG) Incubation of the planted
bacteria
Treatment D: Positive Control
the rug resistance bacteria. It was well known that the drug
drug resistance bacteria may enter the human via food from the
1998)
(Saxena, 1994).
question:
Coli.
C. Statement of the Hypothesis
D. Objectives
General Objective:
Specific Objective:
G. Definition of terms
Pandanus specie
susceptibility testing.
Wiregrass
is between 1 1/2 and 2 1/2 feet. The leaf blade is mostly basal;
1/2 to 3/4 inch long, one a little longer than other two.
Escherichia Coli
20 minutes to reproduce.
Pandanus specie
-
Results and Discussions
Coli.
R1 R2 R3 R4 Mean
mean of 14.75mm.
Table 2. One way Analysis of Variance for the four different
Sum of
Between
235.250 3 78.417 2.865 .081
Groups
Within
328.500 12 27.375
Groups
Total 563.750 15
concludes that:
RECOMMENDATIONS
specie.
BIBLIOGRAPHY
A. Books
Santos, 1995
Elof, 1998
Saxena, 1994
APPENDICES
A. Plates
materials
Escherichia Coli
Incubation of the agar plates
B. Time Table
C o n struction of
t h e Significance
o f the Study,
S c o pe and
L i m itations and
D e f inition of
T e r ms
A u g u s t 1 4, 2 0 1 4 C o n sultation of The task was done
t h e project at
C a p iz Memorial
P r o vincial
H o s pital
August 15, 2014 P r e paration of The task was don e
t h e materials for
the
e x p erimentation
August 16, 2014 P r o ject The task was don e
e x p erimentation
a t Capiz Memorial
P r o vincial
H o s pital
A u g u s t 1 7- 1 8 , The task was don e
M e a sured the
2014
z o n es of
i n h ibition and
g a t hering of data
D i s posal of the
b a c teria
A u g u s t 2 3- 2 4 , A n a lysis and The task was don e
2014 I n t erpretation of
c o l lected data
September 4 , C o n struction of The task was don e
2014 t h e Conclusion,
R e c ommendations,
a n d Bibliography
September 10 , C o n struction of The task was don e
2014 t h e Abstact and
F i n alization of
t h e Study
September 16, F i n al write - ups The task was don e
2014 o f the std was
c o m pletely
f i n alized
A CKNOWLEDGEMENT
encouragement.
her study.
f r i e n d s f o r t h e i r e n c ouragement.