Professional Documents
Culture Documents
3 Smas Workshop NYC Has
3 Smas Workshop NYC Has
The GOOD
(technological
flora)
The BAD
(Pathogens) &
The UGLY
(spoilage flora)
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 1
Agricultural University of Athens
Dept. Food Science & Technology
Laboratory of Microbiology & Biotechnology of Foods
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 2
Microbiology of Spoilage;
Spoilage of chilled food products;
Ecology of Spoilage as a function of process
and packaging;
Quantitative evaluation of spoilage
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 3
What is spoilage
Who is causing spoilage
How we can evaluate/measure spoilage
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 4
What is (meat) spoilage
Who is causing spoilage
How we can evaluate/measure spoilage
Why this valuation/measurement is needed
Where these measurements will be used
When and from whom these measurements will
be applied
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 5
Spoilage of meat can be considered as
an ecological phenomenon that
encompasses changes of the available
components (e.g. low molecular
compounds), during proliferation of
bacterial present in the microbial
association of the stored meat.
Food (meat) spoilage can be considered
as any change which renders a product
(meat) unacceptable for human
consumption (Hayes 1985)
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 6
What it makes Meat Unacceptable
Color defects
Changes in texture
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 7
What is spoilage
Who is causing spoilage
9General aspects of spoilage organisms
9Factors affecting their development
9spoilage ecology as a function of process and packaging
Indigenous enzymes
(Proteolysis )
Raw
Microorganisms (MUSCLE)
(bacteria, yeasts, fungi) meat
(Gram +,-), aerobes,
micro-aerobic, anaerobes
Chemical spoilage (fat
oxidation etc)
Spoilage
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 9
Meat Microbial Ecology
A small fraction will spoil the meat
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 10
Facultative anaerobes Catalase reaction-negative
Shewanella putrefaciens Lactobacillus spp.
S. baltica L. sake
S. oneidensis L. curvatus
Photobacterium phosphereum L. bavaricus
Enterobacteriaceae
Serratia spp. Carnobacterium spp.
S. marcencens C. divergens
S. liquefaciens C. piscicola
Citrobacter spp.
C. freundii Leuconostoc spp.
C. koseri L. carnosum
Providencia aerogenes L. gelidum
Enterobacter spp. L. amelibiosum
E. aerogenes L. mesenteroides
E. cloacae subsp.
E. agllomerans mesenteroides
Hafnia alvei
Kluyera spp. Pediococcus spp.
Morganella morganii
Pantoea agglomerans Weissella spp.
Raoultella spp. W. hellenica
R. planticolla (Kl.
pneumonieae) Lactococcus raffinolactis
Vibrionaceae
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 11
Gram-negative bacteria Gram-positive bacteria
Aerobes Catalase reaction-weak
Neisseriaceae
Psychrobacter immobilis Brochothrix thermosphacta
P. phenylpyruvica Kutrhia zophii
Acinetobacter spp. Staphylococcus spp.
A. twoffii Clostridium estertheticum
A. Johnsonii Clostridium frigidicarnis
Pseudomonadaceae Clostridium casigenes
Pseudomonas rRNA homology Clostridium algidixylanolyticum sp. nov
Group 1
Pseudomonas fluorescens
Biovars I, II, III, IV,V (includes 7
clusters)
P. lundensis,
P .fragi
P. putida
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 12
Meat/Fish
Cardinal Members of the South EU North EU
Microbial association air Vp/ air Vp/
map map
of meat
Pseudomonas spp. ** ** <
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 13
Table 1.1: Minimum (min), maximum (max) and average (mean), of microbiological
flora of ground pork from Athens open market.
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 14
Meat Finfish
Component Pre Post Pre Post
Creatine Phosphate 300 nf 9.3* 0.2*
Creatine 450 650 nd nd
Betaine nf nd nad 100**
ATP 300 nad 6.5* 0.2*
IMP 20 300 nd nd
Glycogen 1000 100 220 40
Glucose 500 100 220 40
Glucose-6-Phosphate 100 200 21 32
Lactic acid 100 900 100 400
pH 7.2 5.5 7.3 6.5
Free Amino acids 200 350 nad 250
TMAO nd nd nd 350-1000
Carnosine & Anserine 300 300 nd 100***
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 15
Substrates used for growth of major meat spoilage microorganisms
A B C D
Glucose 1 1 1 1
glucose-6-P, 2 2
D-L Lactic acid 3 2 3 A: Pseudomonas spp.
Pyruvate, 4 3 B Sh. putrefaciens
Gluconate, 5 4
Gluconate 6-P, 6 C. Br. thermosphacta
proprionate 5 D. Enterobacter spp.
ethanol 6
acetate 7
Amino acids, 7 8 2 4
creatine 8
creatinine 9
citrate, 10
aspartate, 11
glutamate 12
ribose 3
glycerol 4
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 16
Peptides, proteins, fats
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 17
What is spoilage
Who is causing spoilage
9General aspects of spoilage organisms
9Factors affecting their development
9spoilage ecology as a function of process and packaging
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 19
Niche / Domain; Y= Growth X=pH Z=T°C Gram et al. 2002
Ν=available nutrients
Ν
Ν+2=spatial
Ν+1=interaction
Υ
Ν+1 Ν+2
Ζ
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 20
Appl. Environ. Microbiol. 69, 204 Tsigarida et al. (2003)
Growth curves of control cultures of
Pseudomonas sp. strains (A), B.
thermosphacta strains (B), and S.
putrefaciens strains (C) and changes in
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16
th Dec 2005, 21
glucose concentration (P).
Appl. Environ. Microbiol. 69, 204 Tsigarida et al. (2003)
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 22
Appl. Environ. Microbiol. 69, 204 Tsigarida et al. (2003)
A negative response
(antagonistic) can be
regarded also as a factor
governing the selection of
spoilage flora, particularly
of meat. This is the case
with pseudomonads and S.
putrefaciens. It is well
established in the literature
that the inhibitory effect of
the former bacterium on the
latter is attributed to the
ability of the Pseudomonas
sp. to produce siderophores
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 23
Processing factors
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 24
Microbial Association
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 25
Microbial Association
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 26
Gas Composition Specific spoilage flora Specific spoilage flora fish
meat and poultry
Air Pseudomonas spp. Shewanella putrefaciens,
Pseudomonas spp.
>50% CO2 with B. thermosphacta B. thermosphacta, S.
O2 putrfaciens
>50% CO2 ? Enterobacteriaceae Photobacterium
phosphereum
<50%CO2 with Brochothrix Photobacterium
O2 thermosphacta, lactic phosphereum, Lactic acid
acid bacteria bacteria, B. thermosphacta
>50% CO2 ? lactic acid bacteria Lactic acid bacteria
100% CO2 lactic acid bacteria Lactic acid bacteria
Vacuum packaged Pseudomonas spp., B. Pseudomonads
thermosphacta
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 27
Effect of film characteristics (permeability vs % CO2) on
the growth rate of main spoilage bacteria
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 28
What is spoilage
Who is causing spoilage
9General aspects of spoilage organisms
9spoilage ecology as a function of process and packaging
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 29
Organoleptic analysis
Chemical methods
Physical Methods
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 30
Their use in the Meat Industry is under
consideration since their disadvantage are related
with
Time consuming,
Retrospective results,
Few samples,
Limited reproducibility,
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 31
The time consuming microbiological analyses
should be replaced by the chemical changes
which associated with microbial growth on meat
(e.g. microbial metabolites and/or substrates)
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 32
What is spoilage
Who is causing spoilage
9General aspects of spoilage organisms
9spoilage ecology as a function of process and packaging
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 34
What is spoilage
Who is causing spoilage
9General aspects of spoilage organisms
9spoilage ecology as a function of process and packaging
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 36
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 37
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 38
Organoleptic analysis
Chemical methods
Physical Methods
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 39
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 40
What is spoilage
Who is causing spoilage
9General aspects of spoilage organisms
9spoilage ecology as a function of process and packaging
Chemical methods
Physical Methods
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 42
Compound Test Tested Red Finfis
Storage meat & h
conditions poultry
Glucose Enzymatic kit Air, vp, map < <
Acetate Enzymatic kit, vp-map < <
HPLC
gluconate Enzymatic kit Air, vp-map <
Total lactate HPLC vp-map <
D-lactate Enzymatic kit vp-map < <
Ethanol Enzymatic kit, GLC vp-map <
Free amino acids chromatometric Air <
Ammonia Enzymatic, Air < <
colorimetric
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 43
Compound Test Tested Red Finfis
Storage meat & h
conditions poultry
Acetone, methyl GLC vp-map < <
ethyl ketone, GC/MS
dimethyl Sulphur selective
sulphide, detector
dimethyldisulphi
de
Hydrogen
sylfide
diacetyl, acetoin <
colorimetric vp-map
Biogenic amines HPLC, sensors, < <
enzymic test, Air, vp, map
GLC, Enzyme
electrodes, test
strips
Diamines Amperometric Air <
electrodes
(enzymatic
systems)
Microbial Enzymic/Resazuri air < <
activity n
Volatiles Electronic noses, Air-vp-map < <
(odours) PTR-MS
(chemical sensors)
Proteolysis FT-IR, NIR, Air-vp-map < <
(Amides, MIR
amines, etc.)
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 44
Compound Test Tested Red Finfis
Storage meat & h
conditions poultry
Torymeter <
ATP/IMP HPLC air <
K-values HPLC air <
Lipid oxidation HPLC, GC, Air,vp <
spectroscopy
TMA HPLC, GC, air <
sensors,
enzymatic
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 45
The spoilage indicators or microbial metabolites
should meet among others the following criteria
(Jay, 1986a):
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 46
Changes of area of acid withRT6.55
4.5E5
4E5
3.5E5
3E5
peak area
2.5E5
2E5
1.5E5
1E5
50000
t0
t5
0 t10
0 50 100 150 200 250
t15
time
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 47
As far as the spoilage indicators or microbial
metabolites concern it is general accepted that
these should meet among others the following
criteria (Jay, 1986a):
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 48
changes in oa 7.7
4500000
4000000
3500000 20
3000000 15
area (%)
2500000
10
2000000
5
1500000
0
1000000
500000
0
0 50 100 150 200 250
time (h)
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 49
Scatterplot (Spreadsheet2 in t-1a modified 100v*100c)
90000
80000
70000
concentration of Acetic acid in meat
60000
50000
40000
30000
20000
10000 aa-0
aa-5
aa-10
0 aa-15
0 20 40 60 80 100 120 140 160 180 200 220 240 260
aa-20
time
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 50
As far as the spoilage indicators or microbial
metabolites concern it is general accepted that
these should meet among others the following
criteria (Jay, 1986a):
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 51
Scatterplot (Spreadsheet2 in t-1a modified 100v*100c)
90000
80000
70000
concentration of Acetic acid in meat
60000
50000
40000
30000
20000
10000 aa-0
aa-5
aa-10
0 aa-15
0 20 40 60 80 100 120 140 160 180 200 220 240 260
aa-20
time
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 52
The identification of the ideal metabolite that can be used for
spoilage assessment has proved a difficult task:
a) most metabolites maybe specific to certain organisms (e.g.
gluconate to pseudomonads) and when these organisms are not
present or inhibited by the natural or imposed from man, food
ecology, this provides incorrect spoilage information,
b)
b) the
the metabolites
metabolites are
are the
the result
result of
of the
the consumption
consumption of
of aa specif
specific
ic
substrate
substrate but
but the
the absence
absence of
of the
the given
given substrate
substrate or
or its
its presence
presence in in
low
low quantities
quantities does
does not
not preclude
preclude spoilage,
spoilage,
c)
c) the
the rate
rate of
of microbial
microbial metabolite
metabolite production
production and
and the
the metabolic
metabolic
pathways
pathways of
of these
these bacteria
bacteria are
are affected
affected by
by the
the imposed
imposed
environmental
environmental conditions
conditions (e.g.
(e.g. pH,
pH, oxygen
oxygen tension,
tension, temperature
temperature
etc.),
etc.),
d)
d) the
the accurate
accurate detection
detection and
and their
their measurements
measurements requires
requires
sophisticated
sophisticated procedures,
procedures, high
high educated
educated personnel,
personnel, time
time and
and
equipment
equipment and
and
e)
e) many
many of
of them
them give
give aa retrospective
retrospective information
information which
which is
is not
not
satisfactory.
satisfactory.
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 53
Microbial Association
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 54
The identification of the ideal metabolite that can be used for
spoilage assessment has proved a difficult task:
a)
a) most
most metabolites
metabolites maybe
maybe specific
specific to
to certain
certain organisms
organisms (e.g.
(e.g.
gluconate
gluconate to
to pseudomonads
pseudomonads)) and
and when
when these
these organisms
organisms are
are not
not
present
present or
or inhibited
inhibited by
by the
the natural
natural or
or imposed
imposed from
from man,
man, food
food
ecology,
ecology, this
this provides
provides incorrect
incorrect spoilage
spoilage information,
information,
b) the metabolites are the result of the consumption of a specific
substrate but the absence of the given substrate or its presence in
low quantities does not preclude spoilage,
c)
c) the
the rate
rate of
of microbial
microbial metabolite
metabolite production
production and
and the
the metabolic
metabolic
pathways
pathways of
of these
these bacteria
bacteria are
are affected
affected by
by the
the imposed
imposed
environmental
environmental conditions
conditions (e.g.
(e.g. pH,
pH, oxygen
oxygen tension,
tension, temperature
temperature
etc.),
etc.),
d)
d) the
the accurate
accurate detection
detection and
and their
their measurements
measurements requires
requires
sophisticated
sophisticated procedures,
procedures, high
high educated
educated personnel,
personnel, time
time and
and
equipment
equipment and
and
e)
e) many
many of
of them
them give
give aa retrospective
retrospective information
information which
which is
is not
not
satisfactory.
satisfactory.
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 55
The identification of the ideal metabolite that can be used for
spoilage assessment has proved a difficult task:
a)
a) most
most metabolites
metabolites maybe
maybe specific
specific to
to certain
certain organisms
organisms (e.g.
(e.g.
gluconate
gluconate to
to pseudomonads
pseudomonads)) and
and when
when these
these organisms
organisms are
are not
not
present
present or
or inhibited
inhibited by
by the
the natural
natural or
or imposed
imposed from
from man,
man, food
food
ecology,
ecology, this
this provides
provides incorrect
incorrect spoilage
spoilage information,
information,
b)
b) the
the metabolites
metabolites are
are the
the result
result of
of the
the consumption
consumption of
of aa specif
specific
ic
substrate
substrate but
but the
the absence
absence of
of the
the given
given substrate
substrate or
or its
its presence
presence in in
low
low quantities
quantities does
does not
not preclude
preclude spoilage,
spoilage,
c) the rate of microbial metabolite production or consumption and
the metabolic pathways of these bacteria are affected by the
imposed environmental conditions (e.g. pH, oxygen tension,
temperature etc.),
d)
d) the
the accurate
accurate detection
detection and
and their
their measurements
measurements requires
requires
sophisticated
sophisticated procedures,
procedures, high
high educated
educated personnel,
personnel, time
time and
and
equipment
equipment and
and
e)
e) many
many of
of them
them give
give aa retrospective
retrospective information
information which
which is
is not
not
satisfactory.
satisfactory.
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 56
Linear regression of changes of lactic acid in meat stored under different
T° C (: 0 oC, 10 oC, : 15 oC, +: 20 oC)
Air
400 50% CO
2/50% Air
400
350
350
Lactic Acid (%area)
300
150 150
100 100
50 50
0 0
0 50 100 150 200 250 300 350 400 0 100 200 300 400 500 600 700 800
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 57
The identification of the ideal metabolite that can be used for
spoilage assessment has proved a difficult task:
a)
a) most
most metabolites
metabolites maybe
maybe specific
specific to
to certain
certain organisms
organisms (e.g.
(e.g.
gluconate
gluconate to
to pseudomonads
pseudomonads)) and
and when
when these
these organisms
organisms are
are not
not
present
present or
or inhibited
inhibited by
by the
the natural
natural or
or imposed
imposed from
from man,
man, food
food
ecology,
ecology, this
this provides
provides incorrect
incorrect spoilage
spoilage information,
information,
b)
b) the
the metabolites
metabolites are
are the
the result
result of
of the
the consumption
consumption of
of aa specif
specific
ic
substrate
substrate but
but the
the absence
absence of
of the
the given
given substrate
substrate or
or its
its presence
presence in in
low
low quantities
quantities does
does not
not preclude
preclude spoilage,
spoilage,
c)
c) the
the rate
rate of
of microbial
microbial metabolite
metabolite production
production or
or consumption
consumption an
andd
the
the metabolic
metabolic pathways
pathways ofof these
these bacteria
bacteria are
are affected
affected by
by the
the
imposed
imposed environmental
environmental conditions
conditions (e.g.
(e.g. pH,
pH, oxygen
oxygen tension,
tension,
temperature
temperature etc.),
etc.),
d) the accurate detection and their measurements requires
sophisticated procedures, high educated personnel, time and
equipment and
e)
e) many
many of
of them
them give
give aa retrospective
retrospective information
information which
which is
is not
not
satisfactory.
satisfactory.
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 58
Bloodhound BH114
Electronic nose
GASP Instrument
Ai
t (min)
Headspace
Sample
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 59
Spoilage
recognition
sensors
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 60
Typical FT-IR
absorbance spectra
from pre- and
postspoilage chicken.
Also shown is the
Pearson correlation
coefficient (R) between
the FT-IR absorbances
and the log10(TVC).
The asterisks indicate
peaks that are
attributable to amide I
(1,640 cm1), amide II
(1,550 cm1), and amine
(1,240 and 1,088 cm1)
vibrations.
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 61
Generalised schematic
of an electronic nose
employing chemical
sensors.
The pattern-
recognition system, is
based on neural
networks (machine
learning)
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 62
Monitoring the end-products formation and/or
substrate changes occurring during storage of meat
(pork) at different T° C was analyzed with HPLC
Cluster analysis
Factor Analysis
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 63
LA
FA
AA
Cluster Tree
PB
PBS
BS
PS
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 65
Dendrogram using Average Linkage (Within Group)
De n drogram
0.700
0.600
0.500
0.400
Dissimilarity
0.300
0.200
0.100
0
0b
0d
79d
21d
48b
10d
72b
96b
31d
43d
67d
26d
53d
0c
0e
5e
144b
192b
240b
288b
79c
72a
21c
10c
31c
11e
53c
43c
35e
67c
25e
48e
90e
120a
168a
216a
250a
336a
384a
122c
18,5e
67,5e
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 66
Canonical Discriminant Functions
10
6
1
4 Ward Method
Group Centroids
2
2
Ungrouped Cases
0
4
-2 3
3
Function 2
-4 2
-6 1
-6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6
Function 1
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 67
Canonical Discriminant Functions
10
7
8
Ward Method
6 4 Group Centroids
4 7
1
6
2 5 6
5
0
2 4
-2
3
Function 2
3
-4 2
-6 1
-10 0 10 20 30
Function 1
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 68
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 69
Food
Safety &
Quality
Field Tests
(end-users)
P Devices & P
I methods
monitoring I
Microbial status
L safety and L
(Emerging pathogens Metabolomics &
L & Specific Spoilage quality
Genomics L
organisms) and their
A P I L L A R 3 A
Activities in
R products R
1 2
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 70
THANK YOU
“Spoilage of muscle (meat) spoilage” Safety & Shelf life assessment of meat products in Europe-Workshop 16th Dec 2005, 71