Exam 1 MIBO3500 Spring 2013 (35 pts possible on exam ; Percentage recorded in eC) Ey 0 a Short answer: Place your answers in the space provided below. Write legibly and answer the question within. sentence limits to obtain credit. Wrong information will be counted against you. (15 pts total for the section) 21. Proyide TWO reasons why rRNA is used in taxonomic identification schemes. (2 pts) tRNA is present Le all olganivnry TRNA hascthe sate functiar u- alll ovqani¢uys (wales up th ribasomc) _BeBraw an image which represents the term: XE one pole (Oc a) staphylococcus (1 pt) ») bitricHous bacillus (2 pt) Cluster 4 23 Se 23. The image below represents a typical growth curve for a bacterial culture in a closed system. 3 ‘Ee diate decline 4 Ny TE Time a) oe ‘the growth phase identified ” "2’, list its two different names. (1 pt) Exponential" ov "log" phase Explain why the growth phase labeled “1” is drawn as a horizontal line. Use no more than two sentences! Sentences beyond that will be ignored. (1 pt) = Phase | Ly the lag Phare, ar Whick culls ate metabolically active # may le growing m~ size, bur the number of (lls A the LAP Ler 4 not ~ changivg - ‘Would the sequence of a protein itself be a characteristic used in a phéneticlor phylogenetic taxonomic system? (0.5 pts) phylogenetic Contrast the mordant used in the acid fast stain to the one used in the Gram stain, (Hint: 'm not looking for the actual mordant names here, but how they work) Use no more than two sentences! Sentences beyond that will be ignored. (1 pt) The Gram stilh uce Ganrs iodihe chemical mordant. The acid fast ¢ Which ig @ Physical vupydant. pwonick if @regative bacterial cells. (Each row is worth 1 pt; 4 pts total) Cie em Presence of hopanoids? (VES oF NO} Yes iG Contains the target of penicillin t action Ye Yes | (VES or NO) Degree of the effectiveness of ; lysozyme on the bacterial cell? Ve ty area (VERY, MINIMAL or NONE) ~ (still has outerme Outer mum bran | Outermost layer of the cell wall is called Peptidogliycas CUPS is outersExam 1 MIBO3500 Spring 2013, (35 pts possible on exam ; Percentage recorded in eLC) (nn as, 26. Although you're aware of the potential for foodborne pathogens in undercooked ground beef, you've ordered a medium rare burger for lunch at your favorite dive diner. Your burger patty was pulled from the refrigerator at 10 am this morning, then put on the grill a 11:30 arp today, cooked and served to you immediately. Assume that the pathogenic bacterium has a generation time of 15 minutes at room temperature and that there were 25 cells of the pathogen in the raw meat patty when it was pulled from the refrigerator. Be sure to show the general setup of your math work to receive ANY credit and circle your final answer! (Recall: Ny=Nox2") Ng = 2< a) Given the information in the scenario, many times has the population doubled by the time your burger hits the hot grill? (1 pt) 10-1130 = GOminutes 6 Himes | b) How many pathogenic bacteria cells would you a to be present in your burger at 11:30 am? (1 pt) NpeNox 2h = 26x26 =[1b00 celle] ¢} Ifthe infectious dose of the pathogenic bacterium which causes gastroenteritis is 1000 cells and you've eaten your entire undercooked burger at lunch, should you expect to contract gastroenteritis? Assume that cooking a burger to medium rare does not kill any microbes present for this scenario, Explain your reasoning. Use no more than two sentences! Sentences beyond that will be ignored (1 pt) Yes j 1600 cee Will be Precent av Your buiqer acta 40 caLile Ade fo cuteyris- 27. Human pathogens belong to(which cardinal group for temperature? (0.5 pt) mesophilec 28. Using the table provided, compare and/or contrast the four listed characteristics in Gram positive and Gram negative bacterial cells. (Each row is worth 1 pt; 4 pts total) an Gram Positive Gram Negative Presence of hopanoids? (VES oF NO} Yes eG Contains the target of penicilin action Yec Yes (YES or NO) Degree of the effectiveness of lysozyme on the bacterial cell? Ver ) (VERY, MINIMAL or NONE) wuivimal (still has outerme Outer Membrane (URS is outermost Outermost layer of the cell wall is called: Peptidogly cam At the Hite tou Let it, which is Wore thar {nougl-