15BCB0047

D.S.V.CHAITANYA VARMA

1. Get the protein SRP54_mouse from SwissPROT.

FASTA SEQUENCE :-

>sp|P14576|SRP54_MOUSE Signal recognition particle 54 kDa protein OS=Mus

musculus GN=Srp54 PE=1 SV=2
MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAI
DLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSK
LAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFK
NENFEIIIVDTSGRHKQEDSLFEEMLQVSNAIQPDNIVYVMDASIGQACEAQAKAFKDKV
DVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDI
EGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFM
SKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQEL
LTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGL
QSMMRQFQQGAAGNMKGMMGFNNM
In the BLAST output, you will see results from many different species (the species are named
next to each hit in the table of results). Choose 8 of the different species which appear in the
results (including human). Try to avoid selecting mutated genes.
Extract the sequences in FASTA format for the 8 hits you chose.

1)

Mus musculus (house mouse)

FASTA SEQUENCE:-
>P14576.2 RecName: Full=Signal recognition particle 54 kDa protein; Short=SRP54
MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLN
KRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTF
RAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVSN
AIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHID
DFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILG
MIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQEL
LTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQG
AAGNMKGMMGFNNM

2)

Canis lupus familiaris (dog)

FASTA SEQUENCE:-
>P61010.1 RecName: Full=Signal recognition particle 54 kDa protein; Short=SRP54
MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLN
KRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTF
RAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVAN
AIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHID
DFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILG
MIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQEL
LTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQG
AAGNMKGMMGFNNM
3)

Rattus norvegicus (Norway rat)

FASTA SEQUENCE:-
>Q6AYB5.1 RecName: Full=Signal recognition particle 54 kDa protein; Short=SRP54
MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLN
KRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYFYQRKGWKTCLICADTF
RAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVSN
AIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHID
DFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILG
MIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQEL
LTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQG
AAGNMKGMMGFNNM

4)

Geodia cydonium

FASTA SEQUENCE:-
>Q8MZJ6.1 RecName: Full=Signal recognition particle 54 kDa protein; Short=SRP54
MVLADLGRKITTALLPRQRTVINEEVLQAMLKEICTALLEADVNVKLVGKLRQNVRAAIDFEDMGAGLSK
RRIIQTSVFNELCKLLDPGVPVWHPTKGHSNVIMFVGLQGSGKTTTCTKLAYHYQKKGWKTCLVCADTFR
AGAFDQLKQNATKARVPFYGSYTEMDPVVIAQEGVEKFKEDSFEVIIVDTSGRHKQEESLFEEMLQVSQA
IDPDNIIFVMDGTIGQACESQARAFKEKVDVASVIVTKLDGHAKGGGALSAVAATRSPIIFIGTGEHIDE
MEPFKTKPFVSKLLGMGDLEGLMEKVSDLKLDENEELMDKLKHGQFTLRDMYEQFQNIMKMGPFNQIIGM
IPGFSPDFMSKGNERESMAKLKRLMTMMDSMNDGELDHPNGAKLFSKQPGRAARVARGSGTSVREVNELL
KQYSNFSATVKKMGGIKGLFKGGDLGKNVNPSQMAKLNQQMAKMMDPRVLQQMGGMSGLQNMMRQFQQGA
SNMPGFKGK

5)

Solanum lycopersicum (Lycopersicon esculentum)

FASTA SEQUENCE:-
>P49972.1 RecName: Full=Signal recognition particle 54 kDa protein 2;
Short=SRP54
MVLAELGGSISRALQQMSNATIIDEKVLNECLNEITRALLQADVQFKLVRDMTTNIKKIVNLDDLAAGHN
KRRIIQQAVFNELCKILDPGKPSFTPKKGKPSIVMFVGLQGSGKTTTCTKYAYYHQKKGWKPALVCADTF
RAGAFDQLKQNATKAKIPFYGSYTESDPVKIAVDGVETFKKENCDLIIVDTSGRHKQEAALFEEMRQVAE
ATKPDLVIFVMDSSIGQAAFDQAQAFKQSVAVGAVIVTKMDGHAKGGGALSAVAATKSPVIFIGTGEHMD
EFEVFDVKPFVSRLLGMGDWSGFMDKIHEVVPMDQQPELLQKLSEGHFTLRIMYEQFQNILKMGPIGQVF
SMLPGFSAELMPKGRENESQAKIKRYMTMMDSMTNEELDSSNPKLMTDSRIMRIARGSGRQVHEVMDMME
EYKRLAKIWSKMKGLKIPKKGEMSALSRNMNAQHMSKVLPPQMLKQIGGMGGLQNLMKQMGSAKDMMGGM
GGMFGGGDK
6)

Arabidopsis thaliana (thale cress)

FASTA SEQUENCE:-
>P49967.2 RecName: Full=Signal recognition particle 54 kDa protein 3;
Short=SRP54
MVLAELGGRITRAIQQMSNVTIIDEKALNECLNEITRALLQSDVSFPLVKEMQSNIKKIVNLEDLAAGHN
KRRIIEQAIFSELCKMLDPGKPAFAPKKAKASVVMFVGLQGAGKTTTCTKYAYYHQKKGYKPALVCADTF
RAGAFDQLKQNATKAKIPFYGSYTESDPVKIAVEGVDTFKKENCDLIIVDTSGRHKQEASLFEEMRQVAE
ATKPDLVIFVMDSSIGQAAFDQAQAFKQSVAVGAVIITKMDGHAKGGGALSAVAATKSPVIFIGTGEHMD
EFEVFDVKPFVSRLLGMGDWSGFVDKLQEVVPKDQQPELLEKLSQGNFTLRIMYDQFQNILNMGPLKEVF
SMLPGISAEMMPKGHEKESQAKIKRYMTMMDSMTNDELDSSNPKVFNESRMMRIARGSGRQVREVMEMLE
EYKRLAKIWSKMKGLKIPKNGDMSALSRNMNAQHMSKVLPPQMLKQIGGMGGLQSLMKQMGSGKDMMGMF
GGGDK

7)

Hordeum vulgare

FASTA SEQUENCE:-
>P49969.1 RecName: Full=Signal recognition particle 54 kDa protein 2;
Short=SRP54
MVLAQLGGSISRALAQMSNATVIDEKVLGECLNEISRALLQSDVQFKMVRDMQTNIRKIVNLETLAAGTN
KRRIIQQAVFTELCNMLDPGKPAFTPKKGKPSVVMFVGLQGSGKTTTCTKYAYYHQRKGFKPSLVCADTF
RAGAFDQLKQNATKAKIPFYGSYMESDPVKIAVEGLERFRKENSDLIIIDTSGRHKQEAALFEEMRQVAE
ATKPDLVIFVMDGSIGQAAFDQAQAFKQSASVGAVIITKLDGHAKGGGALSAVAATKSPVIFIGTGEHID
EFEIFDVKPFVSRLLGMGDLSGLMDKIQDVMPADQQPELLAKLAEGTFTLRLLYEQFQNLLKMGPIGQVF
SMLPGFSSELMPKGHEKEGQAKIKRYMTIMDSMTAAELDSTNPKLMTESRIIRIARGSGRQIRDVTDMLE
EYKRLAKMWSKMKGLKMPKNGKMSDLSQNLNIQQMTKALPPQVLKQMGGMGGLQALMKQMGGKDMSKMLG
GMGLGGD

8)

Solanum lycopersicum (Lycopersicon esculentum)

FASTA SEQUENCE:-
>P49971.1 RecName: Full=Signal recognition particle 54 kDa protein 1;
Short=SRP54
MVLAQLGGSISRALQQMSNATIIDEKVLNECLNEITRALLQADVQFKLVRDMSTNIKKIVNLEDLAAGHN
KRRIIQQAVYNELCKILDPGKPAFTLKKGKPSVVMFVGLQGSGKTTTCTKYAYHHQKRGWKPALVCADTF
RAGAFDQLKQNATKAKIPFYGSSYTESDPVKIAVDGVETFKKENCDLIIVDTSGRHKQEAALFEEMRQVS
EAQKPDLVIFVMDSSIGQAAFDQAQAFRQSVAVGAVIVTKMDGHAKGGGALSRVAATKSPVIFIGTGEHM
DEFEVFDVKPFVSRLLGMGDLSGLVNKIQDVVPMDQQPELLQKLSEGHFTLRIMYEQFQSMLKMGPLGVF
SMLPGFSAEMMPQGREKESQAKFKRYMTMMDSMTDEELDSTNPKILTESRIMRIARGSGRLVHEVMEMLE
EYKRLAKIFSKMKGLKIPKKGDMSSLSRNMNAQNMSKVLPPQMLKQIGGMGGLQNLMKQMGSAKDMMGMF
GGGGGE
Identify the longest conserved region present in all species.

CADTFRAGAFDQLKQNATKA

Among the 8 species which two are closely related species?

Rattus norvegicus (Norway rat) and Mus musculus (house mouse) are closely
related