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Jurnal Captropil
Jurnal Captropil
Jurnal Captropil
10(3)2013
Abstract:
A simple, rapid and sensitive spectrophotometric method has been developed for the
determination of captopril in aqueous solution. The method is based on reaction of
captopril with 2,3-dichloro 1,4- naphthoquinon(Dichlone) in neutral medium to form
a stable yellow colored product which shows maximum absorption at 347 nm with
molar absorptivity of 5.6 ×103 L.mole-1. cm-1. The proposed method is applied
successfully for determination of captopril in commercial pharmaceutical tablets.
Introduction:
Arterial hypertension is one of the
diseases with major prevalence in the
world, being known that one in six
habitants is affected by this health
problem. Although it is caused by still
unknown factors, some risk factors
contribute to the development of this
Fig. (1). Molecular structure of
pathology, like family history, age,
captopril.
high salt intake, obesity, sedentariness,
alcoholism and stress.[1]
Several types of analytical procedures
The first drug planned to be used for
have been proposed for the analysis of
arterial hypertension treatment was
captopril in pharmaceuticals
captopril,1-[(2S)-3-mercapto-2-
formulations. These procedures include
methylpropionyl]-L-proline (Figure 1),
colorimetry[4- 9] ,fluorimetry [10]
which belongs to the class of
,capillary electrophoresis [11],high-
angiotensin-converting enzyme (ACE)
performanceliquid chromatography
inhibitors. This drug interacts with
(HPLC)[12-15],polarography [16],
ACE due to its similarity with a
voltammetry
dipeptide and the sulphydryl group
[17],coulometry[18],amperometry[19],
also plays an important role, linking
conductometry [20], Potentiometry[
covalently to the zinc atom in the
21- 23 ] and flow injection
enzyme active site. This drug is widely
methods[10, 24, 25] . Some of theses
used mainly for arterial hypertension
procedures are not simple for routine
treatment, but also in diabetic
analysis and required expensive or
nephropathy and congestive cardiac
sophisticated instruments. In this work
insufficiency. [2,3]
a simple analytical producer is
described for captopril determination .
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Effect of surfactants
The effect of amount of different types
of surfactants (cationic, anionic and
non-ionic) on the colour intensity of
captopril complex with dichlone
reagent has been tested. The
experimental data show no useful
results. Therefore, they have not been
incoporated in subsequent steps.
Recommended procedure
Fig. (3) Calibration graph for
Transfer an increasing volumes of the
captopril.
100 µg/ml captopril into 25ml
volumetric flask to cover the range (20
Effect of excipients
– 300) µg. Then 2ml of (1×10-3 M)
The effect of some species
Dichlone were added. The volumes are
commonly present in captopril
completed to the mark with distilled
pharmaceutical formulations (lactose,
water and the absorbance is measured
glucose, sucrose, starch, stearic acid,
at 347 nm against the reagent blank
magnesium stearate and
prepared in the same manner but
microcrystalline cellulose) was
without Captopril.
evaluated in molar quantities showing
that the usual amounts in
pharmaceutical formulations doesn't
interfere in captopril determination by
the proposed procedure.
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:الخالصة
تعتمد الطريقة على. تم تطوير طريقة طيفية بسيطة وسريعة وحساسة لتقدير الكابوتريل في المحلول المائي
نفثوكوينون في محيط متعادل إلعطاء ناتج ملون مستقر يظهراعلى1,4 –ثنائي كلورو2,3 تفاعل الكابوتريل مع
تم تطبيق الطريقة وبنجاح في4-سم.4-مول. لتر241 ×6.5 نانوميتر واالمتصاصية الموالرية213 امتصاص عند
.تقدير الكابوتريل في مستحضره الصيدالني بشكل حبوب
970