Professional Documents
Culture Documents
Hank's Stock Solutions (HBSS)
Hank's Stock Solutions (HBSS)
just buy it. First you make each solution, the premix, and then the final solution. All solutions should be
stored at 4oC.
Stock #1
Stock #2
Stock #3
Stock #4
Stock #5
Hank's Buffered Salt Solution (HBSS) full strength (mix prior to use)
9.9 ml Hank's Premix
0.1 ml Stock #5
0.137 M NaCl
5.4 mM KCl
0.25 mM Na2HPO4
0.1g glucose
0.44 mM KH2PO4
1.3 mM CaCl2
1.0 mM MgSO4
4.2 mM NaHCO3