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Effect Of Different Levels Of GA 3 In Vase

Solution Affects On Post Harvest Quality And
Vase Life Of Gladiolus Cut Spikes Cv...

Article in International Journal of Applied Research and Studies · August 2014

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 Effect Of Different Levels Of GA3 In Vase Solution
Affects On Post Harvest Quality And Vase Life Of
Gladiolus Cut Spikes Cv. American Beauty
ISSN 2319-9725

Jadhav P. B.
ASPEE, Agricultural Research and Development Foundation,
‘ASPEE HOUSE’, B. J. Patel Road, Malad (W), Mumbai (MH)
Sahare H.
Ph.D. Scholar, Dept. of Floriculture and Landscaping, ACHF, NAU, Navsari
(GJ)
Senapati, A. K.
Assist. Professor, Dept. of Post Harvest Technology, ACHF. N.A.U.,
Navsari (GJ)
Patil, N. B.
ASPEE, Agricultural Research and Development Foundation,
‘ASPEE HOUSE’, P.O.Box No. 7602, B. J. Patel Road, Malad (W), Mumbai
(MH)
Dekhane, S. S.
ASPEE, Agricultural Research and Development Foundation,
‘ASPEE HOUSE’, P.O.Box No. 7602, B. J. Patel Road, Malad (W), Mumbai
(MH)
Patel, D. J.
Ex. Principal and Dean, B A, Collage of Agriculture A.A.U., Anand (GJ)

Abstract: The present investigations were carried out at ASPEE, ARDF, Research Lab, TANSA,
during the year 20014-15. The experiment was laid down in Completely Randomized Block Design
(CRBD), consisting six treatments including one control. The spikes of gladiolus were harvested at
tight bud stage, made into bundles of two each and treated with GA3 at different levels and then the
spikes were kept in vase solution. The treatment was T2- 50 ppm GA3 + 10 % sucrose showed 40,
59.33, 105, and 122.33ml, maximum uptake of vase solution at 12, 24, 48, and 60 hrs respectively.
Total number of florets opening per spike (10 florets), at 9th (2 shrivelled florets) and 15th (4.5
shrivelled florets) day’s minimum number of shrivelled florets per spike and maximum vase life
(14.33 days) were also recorded in same treatment.
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1. Introduction:

Gladiolus, botanically named Gladiolus grandiflorus, belongs to the family Irridaceae. It's
native of South Africa and the most popular ornamental bulbous plant, grown for its
fascinating spikes, processing florets of brilliant colour, attractive shapes and various sizes,
which open gradually from vase to the top. It is cultivated all over the country to meet out
increase in cut-flower demand. It has been seen that the gladiolus flowers are highly
perishable commodities and vulnerable to large post harvest losses. It claimed that 70% of
potential losing of quality of cut flower are predetermined at the harvest, while post harvest
factors influence 30% of the quality. Therefore, the present study was conducted to find out
effective vase solution for prolonging the vase-life of gladiolus cut spikes.

2. Materials And Methods:

The present investigations were carried out at ASPEE, ARDF, Research Lab, TANSA during
the year 20014-15. The experiment was laid down in completely Randomized Block Design
(CRBD), consisting six treatments including one control. The treatments were T0 Control
(Distilled water), T1- 25 ppm GA3 + 10 % sucrose, T2- 50 ppm GA3 + 10 % sucrose, T3-75
ppm GA3 + 10 % sucrose, T4-100 ppm GA3 + 10 % sucrose and T5-125 ppm GA3 + 10 %
sucrose. The treatments were assigned in three replications and eight spikes were used in
each replication. The spikes of gladiolus were harvested at tight bud stage, made into bundles
of two each and treated with GA3 at different levels and then the spikes were kept in vase
solution.

3. Effect Of Vase Solution:

The spikes were cut from 25 cm below the lowermost floret. The weight of spikes was
recorded before vase solution treatment and the spikes were placed in different vase solutions
including control (distilled water) post harvest life studies. Observations were recorded on
vase solution uptake, total florets opening, florets shrivelling and vase life.

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4. Results And Discussion:

All vase solution treatments were found superior over control for most of the characters in
terms of post harvest life of gladiolus (table no.1). The maximum vase solution uptake
(122.33 ml), higher number of floret opening (10 No.), minimum shrivelled floret (2 and 4.5
No.) and maximum vase life (14 days) were observed when vase solution treatment was
done with Sucrose 10% + GA3 50 ppm under the treatment T3. This may be due to
exogenous supply of sucrose, which replaced the depleted endogenous carbohydrate utilized
during the post harvest life of flower and thereby enhanced vase life. Similar results were also
reported by Bhatacharjee et al., 1999 and Arora et al., 2001. They also suggested that the
sucrose inhibited the senescence process (less shrivelling in treatment T3). Hence, they
enhance the vase life of cut gladiolus spike. The data in table 1 also indicated that, maximum
floret opening (10 No.), were recorded when vase treatment was done with GA3 50 ppm +
Sucrose 10 % under T3 treatment. This may be due to higher soluble sugars in the buds and
cell division or cell elongation by GA3 (Grower, 2001). The increase in floret longevity might
be due to continuous absorption of vase solution, regular supply of carbohydrate (Table 1).
The increase in floret opening and longevity by vase solution might be due to the increased
osmotic concentration and pressure potential of the petal cells thus improving their water
balance and quality of cut flower spikes. The results were also reported by Halevy (1976).
The sucrose in the pulsing solution was the main source of carbohydrate, which decreased the
water potential and improved the water uptake and fresh weight of the stem in gladiolus
Kofranek and Alevy (1975). The increase of floret opening and longevity by GA3 may be due
to enhancement in cell division and cell elongation by promoting DNA synthesis in cell. This
finding is in close agreement with the findings of Gowda (1985).

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July, 2014 www.ijirs.com Vol3 Issue 7

Total
At 9th At 15th
no.
Vase solution uptake (ml) day total day total
of
no. of no. of Vase
florets
florets florets life
opening
shrivelled shrivelled (days)
48 (No.)
12 hr 24 hr 36 hr 60 hr (No.) per (No.) per
hr per
spike spike
spike
T0- Control 30.33 45.03 47.66 77.5 73.83 6.75 3.5 5.5 10.33

T1- 25 ppm GA3 + 10

67.5 96.5 104.33 9.5 2.5 5.38
% sucrose 36.66 51 13.33

T2- 50 ppm GA3 +

63.16 105 122.33 10 2 4.5
10 % sucrose 40 59.33 14.33

T3- 75 ppm GA3 +

56 99 100 8.58 3 5.5
10 % sucrose 31.66 49 13.66

T4- 100 ppm GA3 +

58.16 82 90 8.5 2.3 5
10 % sucrose 30.33 52.333 13.66

T5- 125 ppm GA3 +

49.66 77.5 86.5 9.5 2.75 5.35
10 % sucrose 31.66 52.666 13.66

1.04 1.51 2.73 4.85 2.94 3.90 2.64 2.12 1.02

CD @ 0.05
1.86 1.39 2.77 3.04 5.00 0.61 0.12 0.19 4.38
CV (%)
Note:- Total volume of vase solution per treatment is 400 ml.

Table 1

5. Acknowledgement:

The authors are grateful to ASPEE, Agricultural Research and Development Foundation for
conducted trails for this work.

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References:

1. Arora, S., Singh, K. and Singh. P. J. 2001. Standardization of post harvest package
technology of cut flowers. Final report of ICAR funded ADHOC project, pp-124.
2. Bhattacharjee, S. K. 1999. Post harvest management of cut flowers, cut foilage and
management of pots plants. J. Ornamental Hort., 2 : 32-39.
3. Gowda, J. V. N. 1985. Effect of gibberellic Acid on growth and flowering of rose, cv.
"Super Star". Indian Rose Annuals, IV : 185-187.
4. Grover J. 2001. Modified atmosphere packaging and storage of cut flowers in
polymeric films. Ph.D. Thesis, P.A.U. Ludhiana.
5. Halevy, H. 1976. Transport and conditioning of cut flowers. Acta Hort. 43: 291-306.
6. Kofranek, A. M. and Alevy, A.H. 1976. Sucrose pulsing of gladiolus stem before
storage to increase spike quality. Amer. Soc. Hort. Sci., 11 : 572-573.

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