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Topic 2: Fresh Tissue Preparation: LAVALLE, Jestin B. BMLS Intern Histopathology
Topic 2: Fresh Tissue Preparation: LAVALLE, Jestin B. BMLS Intern Histopathology
To examine the tissue in the living state, allowing protoplasmic activities such as motion, mitosis, phagocytosis and pinocytosis to be
observed
Teasing / Dissociation – Immersed in a watch glass w/ NSS, carefully dissect or separate, examine:
o Phase contrast
o Bright field – may stained w/ methyl violet or methylene blue
Squash Preparation (Crushing) – small piece (1 mm) is placed in a slide and forcibly compressed with another slide or cover
glass
o Vital stain may be placed at the junction of the slide and cover glass, absorbed through capillary attraction
Streaking – Use of applicator stick or a platinum loop, rapidly and gently applied in a direct or zigzag line in a relatively uniform
distribution
Spreading – Selected portion is transferred and gently spread into a moderately thick film (circular motion)
Pull-Apart – A drop of a secretion or sediment is placed upon the slide Another clean slide is placed over allowing to disperse
evenly over the surface of the two slides. Slide are the pulled apart with a single uninterrupted motion
Touch Preparation (Impression smear) – Surface of a freshly cut piece of tissue is brought into contact and pressed on to surface
of a clean glass slide
o Phase or Light
o Cells are examined without destroying their intercellular relationship, and without separating them from their normal
surroundings
Frozen Section – Slices (10-15um) are cut from a fresh tissue frozen on a microtome with CO2 or a cold chamber
o Recommended in lipid and nervous tissue demonstration
Examination of a dead body to find out the cause of death; “Autopsia” – to see with one own’s eye
Forensic – determine the cause and manner of death, obtains biological specimen
Ex. Police investigation, Toxicology if poisoned