clc

clear
n=1;
a=double(imread(['6_X' num2str(1) '.tif']))';
d=double(zeros(size(a)));
for i=1:n:20
a=double(imread(['6_X' num2str(i) '.tif']))';
d=d+a;
end
avgx=d/20;
d=double(zeros(size(a)));

for i=1:n:20
a=double(imread(['BG_X' num2str(i) '.tif']))';
d=d+a;

end
avgbg=d/20;
d=double(zeros(size(a)));
for i=1:n:5
a=double(imread(['FF_X' num2str(i) '.tif']))';
d=d+a;
end
avgff=d/5;

final=(avgx-avgbg)./(avgff-avgbg);
imagesc(final/200); colormap gray
j1=final(:,900);
j2=final(:,1000);
j3=final(:,1100);
figure
plot(j1)
figure
plot(j2)
figure
plot(j3)