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    Maika Doronela
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    The document discusses an assignment to look for alternatives to vector NTI in a local database and discusses highly conserved regions in proteins that are part of the active site. It also defines domains as conserved parts of a protein sequence and structure that can evolve independently. Additionally, it provides a brief overview of PCR, noting that it makes DNA sequences visible through amplification and produces visible bands, and describes the steps of denaturation, annealing and extension temperatures involved in PCR.

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    The document discusses an assignment to look for alternatives to vector NTI in a local database and discusses highly conserved regions in proteins that are part of the active site. It also defines domains as conserved parts of a protein sequence and structure that can evolve independently. Additionally, it provides a brief overview of PCR, noting that it makes DNA sequences visible through amplification and produces visible bands, and describes the steps of denaturation, annealing and extension temperatures involved in PCR.

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    17 views1 page

    Notes

    Uploaded by

    Maika Doronela
    The document discusses an assignment to look for alternatives to vector NTI in a local database and discusses highly conserved regions in proteins that are part of the active site. It also defines domains as conserved parts of a protein sequence and structure that can evolve independently. Additionally, it provides a brief overview of PCR, noting that it makes DNA sequences visible through amplification and produces visible bands, and describes the steps of denaturation, annealing and extension temperatures involved in PCR.

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    ASSIGNMENT!

    1. look for alternatives for vector NTI in local database

    *most impt regions-highly conserved


    -part of active site

    2. Domain-parts in protein-conserved part of a given protein sequence and structure that can evolve,
    function and exist

    PCR-revolutionary because it makes the sequence visible by the naked eye through amplification,
    nagiging visible ang band

    Denaturation- 94C

    -nagiging single band ang DNA

    Annealing- 50-60C

    -kumakapit ang primer

    Reverse primer-kumakapit sa sense

    Forward orimer- kumakapit sa antisense

    Extension-72C

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