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Microbial Limit Test
Microbial Limit Test
For non-sterile products, we can perform microbial contamination testing using harmonized pharmacopoeia or client-supplied methods to determine
the bio burden within the sample. The microbial examination of non –sterile products is done as under.
MICROBIAL EXAMINATION
Solution A Solution B
0.1 mL MacConkey Sol A Sol A Loopfull smaple 1ml of sample 1 Ml of sample Streak on XLDA
Broth+0.1 sample streak on streak on streak on +25ml of + 25ml of SDA after Incubation
Kept for 42-45’c/48 hrs CA MSA VRBGA SCDA of 30-5’c for 24 hrs
Loopfull sample Incubate 30-35’c Incubate 30-35’c Incubate 30-35’c Incubate 30-35’c Incubate for 20-25’c After incubation
Streak on MA for 72 hrs for 72 hrs for 24 hrs for 5 days for 7 days completed
30-35’C Incubate After incubation After incubation After incubation After incubation After incubation Result on
for 72 hrs completed completed completed completed completed colony counter