2.2.

Methods
In this paper I was honored to have hands on summary-statisticslevel results from data of genome-wide
association studies for different traits of obesity related traits from several resources in order to generatet my
own results to evaluate which I can analyze in relation to my hypothesis(27). Therefore, I used a web
interface and centralized database of health science, named LD Hub, which automates a LD score regression Commented [DD1]: I think a link to LDhub would be
relevant here
analysis pipeline out of the result data in able to estimate the genetic correlations between the phenotype
ADHD and different phenotypes of obesity(28). This very big database includes an innumerous amount of
result data from different genome-wide association studies worldwide which makes it possible to estimate
the wanted genetic correlations. In addition to that, this system gives you a clear overview even when
dealing with large sample sizes(29). LD Hub has some conditions that have to be fulfilled in order to upload
the genome-wide association study (GWAS). As an example, the study should be non-sex-stratified and
predominantly European summary statistics so that the investigated SNPs are only of those who concern the
European populations, as these SNPs may not be considered as SNPs within non-European populations. Commented [DD2]: Do you have a short section describing
the underlying method for LD score regression? – The
Another important condition is a large number of individuals is required which makes sample size very big method requires the same underlying LD structure in the
making it more precise to indicate any genetic correlation between the phenotypes. genome of the two phenotypes that are tested, that is why
only studies based on European individuals should be used.

I chose to investigate different traits of obesity as overweight, obesity class I-III, extreme BMI, and
childhood obesity as well as traits which can be linked to obesity as diabetes mellitus type 2, HDL-
cholesterol, and triglycerides (see Table xxx for references to the studies). In order to find any genetic Commented [DD3]: You included the references in the
table – right? – otherwise add them here
correlation between ADHD and these traits, I uploaded the results from the to-date largest first genome-wide
association study of significant risk loci for ADHD (put in ref to our paper on BioRxiv)which my supervisor
Ms. Ditte Demontis took part in. The study isis genome-wide association study is a big genome-wide meta-
analysis based on a big sample of 55,374 individuals from an international collaboration, among them 20,183
ADHD cases and 35,191 controls from Denmark, North America, China, and other European cohorts. The Commented [DD4]: The summery stat you are using for
the genetic correlations are without the Chinese sample.
results from the ADHD genome-wide association study and the studies of the obesity related traits are based This as only Europeans should be used for LD score
on large sample sizes and thus well-powered analysesWith this huge amount of sample size combined with regression analyses (see my comment above).

the huge amount of genome-wide association studies from the database LD Hub, our result datathe estimated
genetic correlation would hopefully be very precise and give important information about the extend to
which the phenotypes share underlying genetic risk factors. support our process of investigating any genetic
correlation between the mentioned traits.