Problem 1. Let an enzyme inhibitor I be present in the reaction mixturé so that to the reactions: ate a E+sS e ESP there is added the reaction 7 7 E+1 > EI in which EI cannot react with the substrate (competitive inhibition). > Use the steady state assumption on ES and El to show that the reaction velocity is given by: KAEMS) kak, ky 1+—-(/)) +[S; : 7 d+ Oss] Problem 2. Use the steady state approximation on the intermediate (PD)ox to calculate the rate of the reaction of P with D to form (PD): é & & 4 P+D & (PD)ow ¢> (PD)in : : 8 n 7 Problem 3, For the two parallel reactions A+B and A-¥C, show that the activation energy E for the disappearance of A is given in terms of activation energies E1 & E> for the two paths by KE, thE, K+ : E Problem 4. Derive the integrated rate equation for a reaction of 4 order. Derive the expression for the half life of such a reaction. Problem 5. The following constants are obtained for the first-order decomposition of acetone dicarboxylic acid in aqueous solution: wc 0 20 40 60 K/0% st 2.46 47.5 376 5480 (a) Caloulate the activation energy. (6) Calculate the pre-exponential factor A. (c) What is the half-life of this reaction at 80°C? Problem 6. (a) The viscosity of the water changes about 2% per degree at room temperature. ‘What is the activation energy for this process? (b) The activation energy for a reaction is 62.8 ki 1 mol". Calculate k3s%/ kas*c.Problem 7, For acetic acid in dilute aqueous solution at 25°C, time is 1.73 x 10°s for a 0.1M solution, Calculate ky and ky in CHsCOOH CHsCOs" +H Problem 8, Calculate the Michaelis constant Ky and maximum velocity Vx for fumarase when L-malate is the substrate using the Eadie-Hofstee method and make a plot to show how well the Michaelis equation fits the data 1.73 * 10% and’ the relaxation IMj/i0*M | 0.1 0.333 | 10 3.33 10 33.3 100 v 19 42 [ 61 65 72 | 74 69