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Bacillus Cereus SPP ISO 7932
Bacillus Cereus SPP ISO 7932
What is it ?
Bacillus cereus is a Gram-positive, facultative to become ill, although its capacity to form spores
anaerobic, endospore-forming, large-rod, widely guarantees its survival throughout the food chain if
distributed and which can cause consumer optimum temperature and humidity conditions
toxiinfection. prevail to allow it to multiply.
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Where can we find B.cereus? ISO UNE EN ISO 7932:2004
A wide variety of foods, including meats, dairy, 1. Colony counting in Selective isolation BACILLUS
vegetables, and fish have been associated with CEREUS SELECTIVE AGAR BASE (MYP) (Cat. 1343)
the diarrheal-type of food poisoning. The
vomiting-type outbreaks generally have been Sow 0.m1 ml of the initial and subsequent dilutions on
associated with rice products and other starchy Petri dishes with Bacillus Cereus Selective Agar Base,
foods, such as potato, pasta, and cheese products. supplemented with Egg Yolk Emulsion (Cat.5152) and
Food mixtures, such as sauces, puddings, soups, Bacillus Cereus Supplement (Polymyxin) (Cat. 6021).
Preserve and handle raw materials, semi-finished Select 3 isolated colonies and incoulate in depth and surface
and finished products, below 7ºC. on Blood Agar N ° 2 plates prepared with 5-7% sterile
defibrinated blood.
N= M x 1/D
How can we identify it? N= Microorganisms number/g or ml of sample
D= Dilution factor of the selected plate
A variety of methods have been recommended
for the recovery, enumeration, and confirmation Growth Absence
of B. cereus in foods. Liquid Product – No detected < 1 cfu/ml
Solid Product – No detected < 10 cfu/ml
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