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Gel Electrophoresis
Gel Electrophoresis
In order to preform gel electrophoresis one must first place the gel in a box filled with
a buffer solution that can conduct current. A power source is placed to the side of the
box connected by a positive electrode on one side and a negative electrode on the
other. A comb is used to make wells on the negative side of the box in which the
macromolecules will be placed. When the power source is activated the molecules will
migrate to the opposite end of the box with the positive electrode. As the
macromolecules travel at different speeds, they will separate into bands containing a
large number of fragments of the same size.