Professional Documents
Culture Documents
M.tech Syllabus SOA
M.tech Syllabus SOA
An overview of bioseparation. Separation of cells and other insolubles from fermented broth.
Filtration and microfiltration, centrifugation (batch, continuous, basket). Cell disruption: Physical
methods (osmotic shock, grinding with abrasives, solid shear, liquid shear), Chemical methods
(alkali, detergents), Enzymatic methods; Products isolation: Solvent Extraction and adsorption
method, precipitation (ammonium sulphate. Organic solvents, high molecular weight polymers),
chromatographic separation; affinity, size exclusion, Thin layer, ion exchange chromatography.
ultrafiltration, Reverse Osmosis, Electrophoretic separation ; Products polishing: Crystallization
and drying.
Texts/References
1. Michael Shuler and Fikret Kargi, Bioprocess Engineering: Basic
Concepts, 2nd Edition, Prentice Hall, Englewood Cliffs, NJ, 2002.
2. Pauline Doran, Bioprocess engineering principles, 1 Edition, Academic
Press, 1995.
3. Colin Ratledge, Bjorn Kristiansen, Basic Biotechnology, 2 Edition, nd
Texts/References:
1. Kuby, RA Goldsby, Thomas J. Kindt, Barbara, A. Osborne Immunology, 6 th Edition,
Freeman, 2002.
2. Brostoff J, Seaddin JK, Male D, Roitt IM., Clinical Immunology, 6 th Edition, Gower
Medical Publishing, 2002.
3. Janeway et al., Immunobiology, 4th Edition, Current Biology publications. 1999.
4. Paul, Fundamental of Immunology, 4th edition, Lippencott Raven, 1999.
Departmental Elective I
Departmental Elective II
Communicative English
2nd Semester
M.BT-2.1
Text/References:
1. S.B. Primrose, R.M. Twyman and R.W.Old; Principles of Gene
Manipulation. 6th Edition, S.B.University Press, 2001.M.Tech
(Biotechnology & Biochemical Engineering)
2. J. Sambrook and D.W. Russel; Molecular Cloning: A Laboratory
Manual, Vols 1-3, CSHL, 2001.
3. Brown TA, Genomes, 3rd ed. Garland Science 2006
4. Selected papers from scientific journals.
5. Technical Literature from Stratagene, Promega, Novagen, New
England Biolab etc.
M.BT-2.2
Biotechnology of Plant Metabolites (Full marks-100)
Introduction to aspects of plant tissue/cell culture e.g. nutrition and media, callus culture.
Cell suspension culture. Introduction to primary and secondary metabolism, Important
pathway leading to the biosynthesis of secondary metabolites (e.g. serpentine, shikonin,
diosgenin and cardenolids) in plants, Secondary. Metabolic products produced by in vitro
culturing of plant cells, selection of plant cells/tissues for the production of a specific
product, Molecular Pharming. Culture system in secondary plant product biosynthesis-
batch / continuous culture and immobilized plant cells. Biotransformation of precursors
by cell culturing. Extraction and analytical methods for the above four metabolites.
Industries involved in the production of plant secondary metabolites, Potential and future
prospect of the secondary metabolities production by plant cells culture techniques.
References:
Departmental Elective I
Departmental Elective II
1. Callus culture
2. Suspension culture
3. In-vitro production of secondary metabolite
4. Extraction methods for bioactive compounds
5. Characterization of bioactive compound
3rd Semester
M.BT-3.1 (Full marks-100)
Advanced bioinformatics
Sequence databases; Similarity matrices; Pairwise alignment; BLAST;
Statistical significance of alignment; Sequence assembly; multiple
sequence alignment; Clustal; Phylogenetics: distance based approaches,
maximum parsimony. Motif representation: consensus, regular
expressions; PSSMs; Markov models; Regulatory sequence identification
using Meme; Gene finding: composition based finding, sequence motif-
based finding.Representation of molecular structures (DNA, mRNA,
protein), secondary structures, domains and motifs; Structure
classification (SCOP, CATH); Visualization software (Pymol, Rasmol etc.);
Structure databases; Secondary structure prediction; RNA structure
prediction; Mfold; Protein structure prediction by comparative modelling
approaches(homology modelling, threading); Ab initio structure
prediction: force fields, backbone conformer generation by Monte Carlo
approaches, side-chain packing; Energy minimization; Molecular
dynamics; Rosetta; Structure comparison (DALI, VAST etc.); CASP;
Protein-ligand docking; Computer-aided drug design (pharmacophore
identification); QSAR; Protein-Protein interactions
Texts/References:
1. David W. Mount. Bioinformatics: Sequence and Genome Analysis
2nd Edition, CSHL Press, 2004.
2. Baxevanis and F. B. F. Ouellette, Bioinformatics: a practical guide
to the analysis of genes and proteins, 2nd Edition, John Wiley,
2001.
3. Jonathan Pevsner, Bioinformatics and Functional Genomics, 1 st
Industrial biotechnology
References
Departmental Elective I
Seminar
Project I
4th Semester
Project II
Protein engineering
Nano-biotechnology
Computational biotechnology
Industrial pharmacology