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Lesson 48
Lesson 48
Lesson 48
Lesson: Chromatography
Aim :
Learning Outcomes :
1. describe briefly and qualitatively paper, column, thin layer and gas/liquid
chromatography in terms of adsorption and/or partition.
2. describe the use of chromatography as an analytical tool. describe the extensive use
of these methods and gas chromatography in industry and medicine.
Underlying Principles
Differentiation
Questions in the student notes are designed to enable all students to complete the activity.
The pop-up answers are provided for the students to view when they have considered their
responses. Worksheet questions include questions that require recall, understanding and
application of the new concepts learned.
• Activity 1 : Principles of
chromatography
• Activity 5 : Gas-liquid
chromatography
1. Principles of chromatography
1.1 a. 3
2
Rf of first component =
= 0.222
9
4
Rf of second component = = 0.444
9
7
Rf of third component = = 0.778
9
1.2 a. The ions in the solution have different degrees of solubility in the solvent.
They travel up the chromatography paper at different rates and are thus
separated.
b. X = Mn
Y = Zn
Z = Co
28
b. Rf value in solvent A = = 0.70
40
10
Rf value in solvent B = = 0.17
60
c. X = Arginine
5. Gas-liquid chromatography
5.5 cm
5.1 a. Retention time = = 11 seconds
0.5 cm s -1